P67596 (SYW_STRR6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Name:	trpS
Ordered Locus Names:	spr2034

Organism

Streptococcus pneumoniae (strain ATCC BAA-255 / R6) [Reference proteome] [HAMAP]

Taxonomic identifier

171101 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Tryptophan--tRNA ligase HAMAP-Rule MF_00140		PRO_0000136691
Regions
	Motif	12 – 20	9	"HIGH" region HAMAP-Rule MF_00140
	Motif	202 – 206	5	"KMSKS" region HAMAP-Rule MF_00140
Sites
	Binding site	205	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P67596 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: CF987DA3C6D01115	FASTA	341	38,418
	10 20 30 40 50 60 MTKPIILTGD RPTGKLHIGH YVGSLKNRVL LQEEDKYDMF VFLADQQALT DHAKDPQTIV 70 80 90 100 110 120 ESIGNVALDY LAVGLDPNKS TIFIQSQIPE LAELSMYYMN LVSLARLERN PTVKTEISQK 130 140 150 160 170 180 GFGESIPTGF LVYPIAQAAD ITAFKANYVP VGTDQKPMIE QTREIVRSFN NAYNCDVLVE 190 200 210 220 230 240 PEGIYPENER AGRLPGLDGN AKMSKSLNNG IYLADDADTL RKKVMSMYTD PDHIRVEDPG 250 260 270 280 290 300 KIEGNMVFHY LDVFGRPEDA QEIADMKERY QRGGLGDVKT KRYLLEILER ELGPIRERRI 310 320 330 340 EFAKDMGEVY NMIQKGSERA REVAGQTLSE VKGAMGLHYF N

References

[1]

"Genome of the bacterium Streptococcus pneumoniae strain R6." Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S., DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C., Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J., Lee L.N. , Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L., Glass J.I. J. Bacteriol. 183:5709-5717(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-255 / R6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007317 Genomic DNA. Translation: AAL00836.1.
PIR	G98125.
RefSeq	NP_359625.1. NC_003098.1.
3D structure databases
ProteinModelPortal	P67596.
SMR	P67596. Positions 2-339.
ModBase	Search...
Protein-protein interaction databases
STRING	171101.spr2034.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL00836; AAL00836; spr2034.
GeneID	933492.
KEGG	spr:spr2034.
PATRIC	19704272. VBIStrPne107296_2200.
Phylogenomic databases
eggNOG	COG0180.
HOGENOM	HOG000059941.
KO	K01867.
OMA	KYNMFVF.
ProtClustDB	PRK12282.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00140_B. Trp_tRNA_synth_B.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. IPR024109. Trp-tRNA-ligase_bac-type. [Graphical view]
PANTHER	PTHR10055. PTHR10055. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYW_STRR6

Accession

Primary (citable) accession number: P67596
Secondary accession number(s): Q97N42

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program