P67591 (SYW_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Name:	trpS
Ordered Locus Names:	Mb3369c

Organism

Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]

Taxonomic identifier

233413 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › Mycobacterium bovis

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140_B
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00140_B
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00140_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Tryptophan--tRNA ligase HAMAP-Rule MF_00140_B		PRO_0000136652
Regions
	Motif	16 – 25	10	"HIGH" region HAMAP-Rule MF_00140_B
	Motif	201 – 205	5	"KMSKS" region HAMAP-Rule MF_00140_B
Sites
	Binding site	204	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P67591 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 8B6512E39649AEB3	FASTA	336	36,272
	10 20 30 40 50 60 MSTPTGSRRI FSGVQPTSDS LHLGNALGAV AQWVGLQDDH DAFFCVVDLH AITIPQDPEA 70 80 90 100 110 120 LRRRTLITAA QYLALGIDPG RATIFVQSQV PAHTQLAWVL GCFTGFGQAS RMTQFKDKSA 130 140 150 160 170 180 RQGSEATTVG LFTYPVLQAA DVLAYDTELV PVGEDQRQHL ELARDVAQRF NSRFPGTLVV 190 200 210 220 230 240 PDVLIPKMTA KIYDLQDPTS KMSKSAGTDA GLINLLDDPA LSAKKIRSAV TDSERDIRYD 250 260 270 280 290 300 PDVKPGVSNL LNIQSAVTGT DIDVLVDGYA GHGYGDLKKD TAEAVVEFVN PIQARVDELT 310 320 330 ADPAELEAVL AAGAQRAHDV ASKTVQRVYD RLGFLL

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248345 Genomic DNA. Translation: CAD95489.1.
RefSeq	NP_857014.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P67591.
SMR	P67591. Positions 10-335.
ModBase	Search...
Protein-protein interaction databases
STRING	233413.Mb3369c.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD95489; CAD95489; Mb3369c.
GeneID	1093742.
KEGG	mbo:Mb3369c.
PATRIC	18009146. VBIMycBov88188_3701.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0180.
HOGENOM	HOG000059940.
KO	K01867.
OMA	HDCLYCV.
ProtClustDB	PRK00927.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00140_B. Trp_tRNA_synth_B.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. IPR024109. Trp-tRNA-ligase_bac-type. [Graphical view]
PANTHER	PTHR10055. PTHR10055. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYW_MYCBO

Accession

Primary (citable) accession number: P67591
Secondary accession number(s): O53386

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program