Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of tryptophan to tRNA(Trp).UniRule annotation

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei135L-tryptophanUniRule annotation1
Binding sitei186ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 13ATPUniRule annotation3
Nucleotide bindingi19 – 20ATPUniRule annotation2
Nucleotide bindingi147 – 149ATPUniRule annotation3
Nucleotide bindingi195 – 199ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:Z4737, ECs4226
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001366301 – 334Tryptophan--tRNA ligaseAdd BLAST334

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi155864.Z4737

Structurei

3D structure databases

ProteinModelPortaliP67589
SMRiP67589
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 20"HIGH" regionUniRule annotation9
Motifi195 – 199"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31 Bacteria
COG0180 LUCA
HOGENOMiHOG000059940
KOiK01867
OMAiQRMTQFK

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

P67589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKPIVFSGA QPSGELTIGN YMGALRQWVN MQDDYHCIYC IVDQHAITVR
60 70 80 90 100
QDAQKLRKAT LDTLALYLAC GIDPEKSTIF VQSHVPEHAQ LGWALNCYTY
110 120 130 140 150
FGELSRMTQF KDKSARYAEN INAGLFDYPV LMAADILLYQ TNLVPVGEDQ
160 170 180 190 200
KQHLELSRDI AQRFNALYGD IFKVPEPFIP KSGARVMSLL EPTKKMSKSD
210 220 230 240 250
DNRNNVIGLL EDPKSVVKKI KRAVTDSDEP PVVRYDVQNK AGVSNLLDIL
260 270 280 290 300
SAVTGQSIPE LEKQFEGKMY GHLKGEVADA VSGMLTELQE RYHRFRNDEA
310 320 330
FLQQVMKDGA EKASAHASRT LKAVYEAIGF VAKP
Length:334
Mass (Da):37,424
Last modified:October 11, 2004 - v1
Checksum:iDEB972FAFDAF3E28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA Translation: AAG58484.1
BA000007 Genomic DNA Translation: BAB37649.1
PIRiB91157
H86002
RefSeqiNP_312253.1, NC_002695.1
WP_000165543.1, NZ_NOKN01000002.1

Genome annotation databases

EnsemblBacteriaiAAG58484; AAG58484; Z4737
BAB37649; BAB37649; BAB37649
GeneIDi915919
KEGGiece:Z4737
ecs:ECs4226
PATRICifig|386585.9.peg.4412

Similar proteinsi

Entry informationi

Entry nameiSYW_ECO57
AccessioniPrimary (citable) accession number: P67589
Secondary accession number(s): Q8X832
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: April 25, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health