P67587 (SYW_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Name:	trpS
Ordered Locus Names:	BR0142, BS1330_I0142

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP MF_00140_B
Subunit structure	Homodimer By similarity. HAMAP MF_00140_B
Subcellular location	Cytoplasm HAMAP MF_00140_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence AAN29096.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Tryptophan--tRNA ligase HAMAP MF_00140_B		PRO_0000136609
Regions
	Motif	14 – 22	9	"HIGH" region HAMAP MF_00140_B
	Motif	217 – 221	5	"KMSKS" region HAMAP MF_00140_B
Sites
	Binding site	220	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P67587 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: F334502A69B2BD1A	FASTA	355	39,130
	10 20 30 40 50 60 MSTFKPLVFS GVQPTGNLHL GNYLGAIKRW VEVQKTEECI YCVVDMHALT VSPDPVELMQ 70 80 90 100 110 120 STREVTAAFL AAGIDPKKSI VFNQSRVMQH AELAWVFNCV ARIGWMSRMT QFKDKAGKDR 130 140 150 160 170 180 ENASLGLFAY PSLMAADILL YRATAVPVGE DQKQHLELTR DIAQKFNNDY SDRIASLGVG 190 200 210 220 230 240 VDMKVGDEQV SGFFPLTEPM ISGPAMRIMS LRDGTKKMSK SDPSDLSRIN LIDDEDTITK 250 260 270 280 290 300 KIRKAKTDSD GLPSEVDGLE GRPEADNLVG IYAALSSTTK EDVLKEFGGR QFSDLKASLA 310 320 330 340 350 DLAVARLSPI THEMRRLVAD PAHIDSVLRD GGEQAGAIAE QTMRHVRDIV GWLQN

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29096.1. Different initiation. CP002997 Genomic DNA. Translation: AEM17508.1.
RefSeq	NP_697181.2. NC_004310.3.
3D structure databases
ProteinModelPortal	P67587.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1165799.
GenomeReviews	Gene locus BR0142 in contig AE014291_GR.
KEGG	bms:BR0142.
PATRIC	17788550. VBIBruSui107850_0148.
TIGR	BR0142.
Phylogenomic databases
HOGENOM	HBG293263.
PhylomeDB	P67587.
ProtClustDB	PRK00927.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0142-MONOMER.
Family and domain databases
HAMAP	MF_00140_B. Trp_tRNA_synth_B. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-synth. IPR024109. Trp-tRNA-synth_bac-type. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01867.
PANTHER	PTHR10055. Trp_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. TrpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYW_BRUSU

Accession

Primary (citable) accession number: P67587
Secondary accession number(s): G0KBB6, Q8G314, Q8YES1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program