P67516 (RLMH_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase H
EC=2.1.1.177

Alternative name(s):
23S rRNA (pseudouridine1915-N3)-methyltransferase
23S rRNA m3Psi1915 methyltransferase
rRNA (pseudouridine-N3-)-methyltransferase RlmH

Gene names

Name:	rlmH
Ordered Locus Names:	BMEI0211

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA By similarity. HAMAP MF_00658
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N3-methylpseudouridine. HAMAP MF_00658 S-adenosyl-L-methionine + pseudouridine(1915) in 23S rRNA = S-adenosyl-L-homocysteine + N(3)-methylpseudouridine(1915) in 23S rRNA. HAMAP MF_00658
Subunit structure	Homodimer By similarity. HAMAP MF_00658
Subcellular location	Cytoplasm Potential HAMAP MF_00658.
Sequence similarities	Belongs to the RNA methyltransferase RlmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 174	174	Ribosomal RNA large subunit methyltransferase H HAMAP MF_00658		PRO_0000198102

Sequences

	Sequence		Length	Mass (Da)
	P67516 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 6888974DB99771A6	FASTA	174	18,649
	10 20 30 40 50 60 MRVSVFAVGR MKSGPERELV ERYFDRFAKA GPPLGLEFAG VSEIPESRGQ TAQLRKAEEA 70 80 90 100 110 120 QRIHEALDNA KSGGAKSGGT SSGGAALILL DERGKTLGSE AFAAIVGRMR DDGKRQLIVA 130 140 150 160 170 IGGPDGHDPA LRSRADLVLA LGELTWPHQI ARILIAEQLY RAATILAGHP YHRS

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51393.1.
PIR	AF3278.
RefSeq	NP_539129.1. NC_003317.1.
3D structure databases
ProteinModelPortal	P67516.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1195923.
GenomeReviews	Gene locus BMEI0211 in contig AE008917_GR.
KEGG	bme:BMEI0211.
PATRIC	17802032. VBIBruMel146950_3333.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG622807.
OMA	ILSGHPY.
PhylomeDB	P67516.
ProtClustDB	PRK00103.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0211-MONOMER.
Family and domain databases
HAMAP	MF_00658. 23SrRNA_methyltr_H. [Tree]
InterPro	IPR003742. SPOUT_MeTrfase. [Graphical view]
KO	K00783.
Pfam	PF02590. SPOUT_MTase. 1 hit. [Graphical view]
PIRSF	PIRSF004505. MT_bac. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMH_BRUME

Accession

Primary (citable) accession number: P67516
Secondary accession number(s): Q8FYM5, Q8YJ75

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program