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P67506

- TRMB_STRPN

UniProt

P67506 - TRMB_STRPN

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei44 – 441S-adenosyl-L-methionineBy similarity
    Binding sitei69 – 691S-adenosyl-L-methionine
    Binding sitei96 – 961S-adenosyl-L-methionineBy similarity
    Active sitei118 – 1181By similarity
    Binding sitei118 – 1181S-adenosyl-L-methionineBy similarity
    Binding sitei122 – 1221SubstrateBy similarity
    Binding sitei154 – 1541SubstrateBy similarity

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSPNE170187:GHGN-555-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:trmB
    Ordered Locus Names:SP_0550
    OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
    Taxonomic identifieri170187 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000000585: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 211211tRNA (guanine-N(7)-)-methyltransferasePRO_0000171403Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi170187.SP_0550.

    Structurei

    Secondary structure

    1
    211
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 146
    Turni17 – 193
    Helixi24 – 263
    Turni27 – 304
    Helixi31 – 355
    Beta strandi41 – 466
    Helixi51 – 599
    Beta strandi63 – 708
    Helixi72 – 8514
    Beta strandi88 – 947
    Helixi100 – 1023
    Beta strandi110 – 1167
    Helixi123 – 1286
    Helixi133 – 14210
    Beta strandi148 – 1547
    Helixi156 – 16914
    Beta strandi172 – 1798
    Helixi180 – 1823
    Helixi192 – 1965
    Helixi198 – 2003
    Beta strandi205 – 2106

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1YZHX-ray2.02A/B1-211[»]
    ProteinModelPortaliP67506.
    SMRiP67506. Positions 6-211.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP67506.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni124 – 1296Interaction with RNASequence Analysis
    Regioni191 – 1944Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000251689.
    KOiK03439.
    OMAiDNVMTEY.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P67506-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVRNRKGAT ELLEANPQYV VLNPLEAKAK WRDLFGNDNP IHVEVGSGKG    50
    AFVSGMAKQN PDINYIGIDI QKSVLSYALD KVLEVGVPNI KLLWVDGSDL 100
    TDYFEDGEID RLYLNFSDPW PKKRHEKRRL TYKTFLDTFK RILPENGEIH 150
    FKTDNRGLFE YSLVSFSQYG MKLNGVWLDL HASDFEGNVM TEYEQKFSNK 200
    GQVIYRVEAE F 211
    Length:211
    Mass (Da):24,379
    Last modified:October 11, 2004 - v1
    Checksum:iE6155B8C5E9C4485
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005672 Genomic DNA. Translation: AAK74707.1.
    PIRiB95064.
    RefSeqiNP_345067.1. NC_003028.3.

    Genome annotation databases

    EnsemblBacteriaiAAK74707; AAK74707; SP_0550.
    GeneIDi930494.
    KEGGispn:SP_0550.
    PATRICi19705455. VBIStrPne105772_0573.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005672 Genomic DNA. Translation: AAK74707.1 .
    PIRi B95064.
    RefSeqi NP_345067.1. NC_003028.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1YZH X-ray 2.02 A/B 1-211 [» ]
    ProteinModelPortali P67506.
    SMRi P67506. Positions 6-211.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 170187.SP_0550.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK74707 ; AAK74707 ; SP_0550 .
    GeneIDi 930494.
    KEGGi spn:SP_0550.
    PATRICi 19705455. VBIStrPne105772_0573.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000251689.
    KOi K03439.
    OMAi DNVMTEY.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci SPNE170187:GHGN-555-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P67506.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-334 / TIGR4.
    2. "Crystal structure of the conserved methyltransferase from Streptococcus pneumoniae TIGR4."
      Midwest center for structural genomics (MCSG)
      Submitted (APR-2005) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.02 ANGSTROMS).

    Entry informationi

    Entry nameiTRMB_STRPN
    AccessioniPrimary (citable) accession number: P67506
    Secondary accession number(s): Q8DQV7, Q97S62
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3