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P67503 (TRMB_STRA5) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:SAG0376
OrganismStreptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R) [Complete proteome] [HAMAP]
Taxonomic identifier208435 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length211 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 211211tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171399

Regions

Region124 – 1296Interaction with RNA Potential
Region191 – 1944Substrate binding By similarity

Sites

Active site1181 By similarity
Binding site441S-adenosyl-L-methionine By similarity
Binding site691S-adenosyl-L-methionine By similarity
Binding site961S-adenosyl-L-methionine By similarity
Binding site1181S-adenosyl-L-methionine By similarity
Binding site1221Substrate By similarity
Binding site1541Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P67503 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 6BA243DFC99EB213

FASTA21124,477
        10         20         30         40         50         60 
MRVRKRKGAE EHLENNPHYV ISNPEEAKGR WHEIFGNNNP IHIEVGSGKG AFITGMAEQN 

        70         80         90        100        110        120 
PDINYIGIDI QLSVLSYALD KVLDSGAKNI KLLLVDGSSL SNYFDTGEVD LMYLNFSDPW 

       130        140        150        160        170        180 
PKKKHEKRRL TYKTFLDTYK DILPEQGEIH FKTDNRGLFE YSLASFSQYG MTLKQVWLDL 

       190        200        210 
HASDYQQNIM TEYERKFSNK GQVIYRVEAR F 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009948 Genomic DNA. Translation: AAM99282.1.
RefSeqNP_687410.1. NC_004116.1.

3D structure databases

ProteinModelPortalP67503.
SMRP67503. Positions 6-211.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208435.SAG0376.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM99282; AAM99282; SAG0376.
GeneID1013170.
KEGGsag:SAG0376.
PATRIC19627537. VBIStrAga72745_0371.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMAKTDDDEL.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycSAGA208435:GHVY-456-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_STRA5
AccessionPrimary (citable) accession number: P67503
Secondary accession number(s): Q8E1H8, Q8E6Z5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways