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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44S-adenosyl-L-methionineUniRule annotation1
Binding sitei69S-adenosyl-L-methionineUniRule annotation1
Binding sitei96S-adenosyl-L-methionineUniRule annotation1
Active sitei118By similarity1
Binding sitei118S-adenosyl-L-methionineUniRule annotation1
Binding sitei122SubstrateUniRule annotation1
Binding sitei154SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSAGA208435:G1FZP-456-MONOMER
UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:SAG0376
OrganismiStreptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R)
Taxonomic identifieri208435 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000821 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001713991 – 211tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST211

Structurei

3D structure databases

ProteinModelPortaliP67503
SMRiP67503
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 129Interaction with RNAUniRule annotation6
Regioni191 – 194Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000251689
KOiK03439
OMAiWITFPDP

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

P67503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVRKRKGAE EHLENNPHYV ISNPEEAKGR WHEIFGNNNP IHIEVGSGKG
60 70 80 90 100
AFITGMAEQN PDINYIGIDI QLSVLSYALD KVLDSGAKNI KLLLVDGSSL
110 120 130 140 150
SNYFDTGEVD LMYLNFSDPW PKKKHEKRRL TYKTFLDTYK DILPEQGEIH
160 170 180 190 200
FKTDNRGLFE YSLASFSQYG MTLKQVWLDL HASDYQQNIM TEYERKFSNK
210
GQVIYRVEAR F
Length:211
Mass (Da):24,477
Last modified:October 11, 2004 - v1
Checksum:i6BA243DFC99EB213
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009948 Genomic DNA Translation: AAM99282.1
RefSeqiNP_687410.1, NC_004116.1
WP_001266024.1, NC_004116.1

Genome annotation databases

EnsemblBacteriaiAAM99282; AAM99282; SAG0376
GeneIDi1013170
KEGGisag:SAG0376
PATRICifig|208435.3.peg.371

Similar proteinsi

Entry informationi

Entry nameiTRMB_STRA5
AccessioniPrimary (citable) accession number: P67503
Secondary accession number(s): Q8E1H8, Q8E6Z5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: February 28, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome