P67500 (TRMB_STAAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	SAV1748

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057		PRO_0000171389
Regions
	Region	190 – 193	4	Substrate binding Potential
Sites
	Binding site	43	1	S-adenosyl-L-methionine By similarity
	Binding site	68	1	S-adenosyl-L-methionine By similarity
	Binding site	95	1	S-adenosyl-L-methionine By similarity
	Binding site	117	1	S-adenosyl-L-methionine By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	153	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	P67500 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 6E0EA5A49A3FF264	FASTA	214	25,291
	10 20 30 40 50 60 MRVRYKPWAE DYLKDHPELV DMEGQHAGEM TEWFDKTQPI HIEIGSGMGQ FITTLAAQNP 70 80 90 100 110 120 HINYISMERE KSIVYKVLDK VKEMGLTNLK IICNDAIELN EYFKDGEVSR IYLNFSDPWP 130 140 150 160 170 180 KNRHAKRRLT YHTFLALYQQ ILNDEGDLHF KTDNRGLFAY SLESMSQFGM YFTKINLNLH 190 200 210 QEDDGSNILT EYEKKFSDKG SRIYRMEAKF HSQK

References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Mu50 / ATCC 700699.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000017 Genomic DNA. Translation: BAB57910.1.
RefSeq	NP_372272.1. NC_002758.2.
3D structure databases
ProteinModelPortal	P67500.
SMR	P67500. Positions 9-210.
ModBase	Search...
Protein-protein interaction databases
STRING	158878.SAV1748.
2D gel databases
World-2DPAGE	0002:P67500.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB57910; BAB57910; SAV1748.
GeneID	1121723.
KEGG	sav:SAV1748.
PATRIC	19564252. VBIStaAur52173_1804.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0220.
KO	K03439.
OMA	RLRNKPW.
ProtClustDB	PRK00121.
Enzyme and pathway databases
BioCyc	SAUR158878:GJJ5-1764-MONOMER.
UniPathway	UPA00989.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB.
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_STAAM

Accession

Primary (citable) accession number: P67500
Secondary accession number(s): Q99TB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program