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P67500 (TRMB_STAAM) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:SAV1748
OrganismStaphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]
Taxonomic identifier158878 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length214 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 214214tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171389

Regions

Region190 – 1934Substrate binding By similarity

Sites

Active site1171 By similarity
Binding site431S-adenosyl-L-methionine By similarity
Binding site681S-adenosyl-L-methionine By similarity
Binding site951S-adenosyl-L-methionine By similarity
Binding site1171S-adenosyl-L-methionine By similarity
Binding site1211Substrate By similarity
Binding site1531Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P67500 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 6E0EA5A49A3FF264

FASTA21425,291
        10         20         30         40         50         60 
MRVRYKPWAE DYLKDHPELV DMEGQHAGEM TEWFDKTQPI HIEIGSGMGQ FITTLAAQNP 

        70         80         90        100        110        120 
HINYISMERE KSIVYKVLDK VKEMGLTNLK IICNDAIELN EYFKDGEVSR IYLNFSDPWP 

       130        140        150        160        170        180 
KNRHAKRRLT YHTFLALYQQ ILNDEGDLHF KTDNRGLFAY SLESMSQFGM YFTKINLNLH 

       190        200        210 
QEDDGSNILT EYEKKFSDKG SRIYRMEAKF HSQK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000017 Genomic DNA. Translation: BAB57910.1.
RefSeqNP_372272.1. NC_002758.2.

3D structure databases

ProteinModelPortalP67500.
SMRP67500. Positions 9-210.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING158878.SAV1748.

2D gel databases

World-2DPAGE0002:P67500.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB57910; BAB57910; SAV1748.
GeneID1121723.
KEGGsav:SAV1748.
PATRIC19564252. VBIStaAur52173_1804.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMADNVMTEY.
OrthoDBEOG6K6VBC.
PhylomeDBP67500.

Enzyme and pathway databases

BioCycSAUR158878:GJJ5-1764-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_STAAM
AccessionPrimary (citable) accession number: P67500
Secondary accession number(s): Q99TB6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: July 9, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways