P67498 (TRMB_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	Rv0208c, MT0218
ORF Names:	MTCY08D5.03c

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057
Miscellaneous	Was identified as a high-confidence drug target. HAMAP-Rule MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC growth Inferred from mutant phenotype PubMed 12657046. Source: MTBBASE
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057		PRO_0000171361
Regions
	Region	232 – 235	4	Substrate binding Potential
Sites
	Binding site	82	1	S-adenosyl-L-methionine By similarity
	Binding site	107	1	S-adenosyl-L-methionine By similarity
	Binding site	136	1	S-adenosyl-L-methionine By similarity
	Binding site	159	1	S-adenosyl-L-methionine By similarity
	Binding site	163	1	Substrate By similarity
	Binding site	195	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	P67498 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 5CECF14C9650AF07	FASTA	263	28,547
	10 20 30 40 50 60 MVHHGQMHAQ PGVGLRPDTP VASGQLPSTS IRSRRSGISK AQRETWERLW PELGLLALPQ 70 80 90 100 110 120 SPRGTPVDTR AWFGRDAPVV LEIGSGSGTS TLAMAKAEPH VDVIAVDVYR RGLAQLLCAI 130 140 150 160 170 180 DKVGSDGINI RLILGNAVDV LQHLIAPDSL CGVRVFFPDP WPKARHHKRR LLQPATMALI 190 200 210 220 230 240 ADRLVPSGVL HAATDHPGYA EHIAAAGDAE PRLVRVDPDT ELLPISVVRP ATKYERKAQL 250 260 GGGAVIELLW KKHGCSERDL KIR

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842572 Genomic DNA. Translation: CAB07003.1. AE000516 Genomic DNA. Translation: AAK44439.1. AL123456 Genomic DNA. Translation: CCP42936.1.
PIR	F70959.
RefSeq	NP_214722.1. NC_000962.3. NP_334625.1. NC_002755.2. YP_006513530.1. NC_018143.1.
3D structure databases
ProteinModelPortal	P67498.
SMR	P67498. Positions 47-235.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv0208c.
Proteomic databases
PRIDE	P67498.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK44439; AAK44439; MT0218.
GeneID	13316193. 886740. 923113.
KEGG	mtc:MT0218. mtu:Rv0208c. mtv:RVBD_0208c.
PATRIC	18122205. VBIMycTub22151_0237.
Organism-specific databases
TubercuList	Rv0208c.
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000073968.
KO	K03439.
OMA	KFEDKAH.
ProtClustDB	PRK00121.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB.
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_MYCTU

Accession

Primary (citable) accession number: P67498
Secondary accession number(s): L0T600, P96390

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program