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P67476 (ALF_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fructose-bisphosphate aldolase

Short name=FBP aldolase
Short name=FBPA
EC=4.1.2.13
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene names
Name:fba
Ordered Locus Names:Mb0370c
OrganismMycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]
Taxonomic identifier233413 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis By similarity.

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactor

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Sequence similarities

Belongs to the class II fructose-bisphosphate aldolase family.

Ontologies

Keywords
   Biological processGlycolysis
   LigandMetal-binding
Zinc
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionfructose-bisphosphate aldolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344Fructose-bisphosphate aldolase
PRO_0000178723

Regions

Region253 – 2553Dihydroxyacetone phosphate binding By similarity
Region274 – 2774Dihydroxyacetone phosphate binding By similarity

Sites

Active site951Proton donor By similarity
Metal binding961Zinc 1; catalytic By similarity
Metal binding1311Zinc 2 By similarity
Metal binding1611Zinc 2 By similarity
Metal binding2121Zinc 1; catalytic By similarity
Metal binding2521Zinc 1; catalytic By similarity
Binding site531Glyceraldehyde 3-phosphate By similarity
Binding site2131Dihydroxyacetone phosphate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
P67476 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 04FA3E1123F72FB1

FASTA34436,544
        10         20         30         40         50         60 
MPIATPEVYA EMLGQAKQNS YAFPAINCTS SETVNAAIKG FADAGSDGII QFSTGGAEFG 

        70         80         90        100        110        120 
SGLGVKDMVT GAVALAEFTH VIAAKYPVNV ALHTDHCPKD KLDSYVRPLL AISAQRVSKG 

       130        140        150        160        170        180 
GNPLFQSHMW DGSAVPIDEN LAIAQELLKA AAAAKIILEI EIGVVGGEED GVANEINEKL 

       190        200        210        220        230        240 
YTSPEDFEKT IEALGAGEHG KYLLAATFGN VHGVYKPGNV KLRPDILAQG QQVAAAKLGL 

       250        260        270        280        290        300 
PADAKPFDFV FHGGSGSLKS EIEEALRYGV VKMNVDTDTQ YAFTRPIAGH MFTNYDGVLK 

       310        320        330        340 
VDGEVGVKKV YDPRSYLKKA EASMSQRVVQ ACNDLHCAGK SLTH 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248335 Genomic DNA. Translation: CAD93233.1.
RefSeqNP_854033.1. NC_002945.3.

3D structure databases

ProteinModelPortalP67476.
SMRP67476. Positions 2-344.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233413.Mb0370c.

Chemistry

BindingDBP67476.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD93233; CAD93233; Mb0370c.
GeneID1091279.
KEGGmbo:Mb0370c.
PATRIC18002472. VBIMycBov88188_0405.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0191.
HOGENOMHOG000227794.
KOK01624.
OMAKKAYDPR.
OrthoDBEOG69GZPB.
ProtClustDBPRK09197.

Enzyme and pathway databases

UniPathwayUPA00109; UER00183.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALF_MYCBO
AccessionPrimary (citable) accession number: P67476
Secondary accession number(s): O06313
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: December 11, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways