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Protein

Xanthine permease XanQ

Gene

xanQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specific, proton motive force-dependent high-affinity transporter for xanthine.1 Publication

Enzyme regulationi

Inhibited by CCCP and N-ethylmaleimide.1 Publication

Kineticsi

  1. KM=4.2 µM for xanthine1 Publication
  1. Vmax=6.36 nmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei31 – 311Essential for affinity and specificity
Sitei93 – 931Essential for affinity and specificity
Sitei272 – 2721Essential for purine uptake
Sitei304 – 3041Essential for purine uptake
Sitei430 – 4301Important for purine uptake and affinity
Sitei432 – 4321Important for purine uptake and affinity

GO - Molecular functioni

  • xanthine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • xanthine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:YGFO-MONOMER.
ECOL316407:JW2850-MONOMER.
MetaCyc:YGFO-MONOMER.

Protein family/group databases

TCDBi2.A.40.4.3. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Xanthine permease XanQ
Gene namesi
Name:xanQ
Synonyms:ygfO
Ordered Locus Names:b2882, JW2850
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13065. xanQ.

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2525CytoplasmicSequence analysisAdd
BLAST
Transmembranei26 – 4621HelicalSequence analysisAdd
BLAST
Topological domaini47 – 559PeriplasmicSequence analysis
Transmembranei56 – 7621HelicalSequence analysisAdd
BLAST
Topological domaini77 – 804CytoplasmicSequence analysis
Transmembranei81 – 10121HelicalSequence analysisAdd
BLAST
Topological domaini102 – 12019PeriplasmicSequence analysisAdd
BLAST
Transmembranei121 – 14121HelicalSequence analysisAdd
BLAST
Topological domaini142 – 15110CytoplasmicSequence analysis
Transmembranei152 – 17221HelicalSequence analysisAdd
BLAST
Topological domaini173 – 1808PeriplasmicSequence analysis
Transmembranei181 – 20121HelicalSequence analysisAdd
BLAST
Topological domaini202 – 2109CytoplasmicSequence analysis
Transmembranei211 – 23121HelicalSequence analysisAdd
BLAST
Topological domaini232 – 25827PeriplasmicSequence analysisAdd
BLAST
Transmembranei259 – 27921HelicalSequence analysisAdd
BLAST
Topological domaini280 – 29819CytoplasmicSequence analysisAdd
BLAST
Transmembranei299 – 31921HelicalSequence analysisAdd
BLAST
Topological domaini320 – 34223PeriplasmicSequence analysisAdd
BLAST
Transmembranei343 – 36321HelicalSequence analysisAdd
BLAST
Topological domaini364 – 3641CytoplasmicSequence analysis
Transmembranei365 – 38420HelicalSequence analysisAdd
BLAST
Topological domaini385 – 42541PeriplasmicSequence analysisAdd
BLAST
Transmembranei426 – 44621HelicalSequence analysisAdd
BLAST
Topological domaini447 – 46620CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi31 – 311H → C or L: Decrease in activity. 1 Publication
Mutagenesisi31 – 311H → K or R: Strong decrease in activity. 1 Publication
Mutagenesisi31 – 311H → N: No change in activity. 1 Publication
Mutagenesisi31 – 311H → Q: Increase of activity. 1 Publication
Mutagenesisi93 – 931N → A: Highly active. 1 Publication
Mutagenesisi93 – 931N → C, D or T: Strong decrease in activity. 1 Publication
Mutagenesisi93 – 931N → Q: Loss of activity. 1 Publication
Mutagenesisi93 – 931N → S: Increase of activity. 1 Publication
Mutagenesisi164 – 1641K → C or R: Highly active. 1 Publication
Mutagenesisi232 – 2321D → C: Decrease in activity. 1 Publication
Mutagenesisi232 – 2321D → E: Highly active. 1 Publication
Mutagenesisi258 – 2581Q → C: Decrease in activity. 1 Publication
Mutagenesisi258 – 2581Q → N: Highly active. 1 Publication
Mutagenesisi272 – 2721E → C: Loss of activity. 1 Publication
Mutagenesisi272 – 2721E → D: Strong decrease in activity. 1 Publication
Mutagenesisi304 – 3041D → C: Loss of activity. 1 Publication
Mutagenesisi304 – 3041D → E: Strong decrease in activity. 1 Publication
Mutagenesisi385 – 3851R → C: Decrease in activity. 1 Publication
Mutagenesisi385 – 3851R → K: Highly active. 1 Publication
Mutagenesisi421 – 4211P → C: Strong decrease in activity. 1 Publication
Mutagenesisi421 – 4211P → G: Highly active. 1 Publication
Mutagenesisi423 – 4231S → C: Highly active. 1 Publication
Mutagenesisi424 – 4241I → C: Highly active. 1 Publication
Mutagenesisi425 – 4251Y → C: Strong decrease in activity. 1 Publication
Mutagenesisi427 – 4271L → C: Decrease in activity. 1 Publication
Mutagenesisi430 – 4301N → T: Loss of activity. 1 Publication
Mutagenesisi432 – 4321I → A, S, T or V: Strong decrease in activity. 1 Publication
Mutagenesisi432 – 4321I → L, M, E or F: Loss of activity. 1 Publication
Mutagenesisi432 – 4321I → N or Q: Highly active. 1 Publication
Mutagenesisi436 – 4361G → C: Highly active. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Xanthine permease XanQPRO_0000165965Add
BLAST

Proteomic databases

PaxDbiP67444.

Interactioni

Protein-protein interaction databases

BioGridi4261448. 20 interactions.
STRINGi511145.b2882.

Structurei

3D structure databases

ProteinModelPortaliP67444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C2W. Bacteria.
COG2233. LUCA.
HOGENOMiHOG000038198.
InParanoidiP67444.
KOiK16346.
OMAiEGPAYLQ.
OrthoDBiEOG6M6JJK.
PhylomeDBiP67444.

Family and domain databases

InterProiIPR006042. Xan_ur_permease.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00801. ncs2. 1 hit.
PROSITEiPS01116. XANTH_URACIL_PERMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P67444-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDINHAGSD LIFELEDRPP FHQALVGAIT HLLAIFVPMV TPALIVGAAL
60 70 80 90 100
QLSAETTAYL VSMAMIASGI GTWLQVNRYG IVGSGLLSIQ SVNFSFVTVM
110 120 130 140 150
IALGSSMKSD GFHEELIMSS LLGVSFVGAF LVVGSSFILP YLRRVITPTV
160 170 180 190 200
SGIVVLMIGL SLIKVGIIDF GGGFAAKSSG TFGNYEHLGV GLLVLIVVIG
210 220 230 240 250
FNCCRSPLLR MGGIAIGLCV GYIASLCLGM VDFSSMRNLP LITIPHPFKY
260 270 280 290 300
GFSFSFHQFL VVGTIYLLSV LEAVGDITAT AMVSRRPIQG EEYQSRLKGG
310 320 330 340 350
VLADGLVSVI ASAVGSLPLT TFAQNNGVIQ MTGVASRYVG RTIAVMLVIL
360 370 380 390 400
GLFPMIGGFF TTIPSAVLGG AMTLMFSMIA IAGIRIIITN GLKRRETLIV
410 420 430 440 450
ATSLGLGLGV SYDPEIFKIL PASIYVLVEN PICAGGLTAI LLNIILPGGY
460
RQENVLPGIT SAEEMD
Length:466
Mass (Da):49,108
Last modified:December 4, 2007 - v2
Checksum:i95EAFB06FEEE9175
GO

Sequence cautioni

The sequence AAA83063.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83063.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75920.2.
AP009048 Genomic DNA. Translation: BAE76948.1.
PIRiB65072.
RefSeqiNP_417358.2. NC_000913.3.
WP_001280192.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC75920; AAC75920; b2882.
BAE76948; BAE76948; BAE76948.
GeneIDi949075.
KEGGiecj:JW2850.
eco:b2882.
PATRICi32121174. VBIEscCol129921_2975.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83063.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75920.2.
AP009048 Genomic DNA. Translation: BAE76948.1.
PIRiB65072.
RefSeqiNP_417358.2. NC_000913.3.
WP_001280192.1. NZ_CP014272.1.

3D structure databases

ProteinModelPortaliP67444.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261448. 20 interactions.
STRINGi511145.b2882.

Protein family/group databases

TCDBi2.A.40.4.3. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Proteomic databases

PaxDbiP67444.

Protocols and materials databases

DNASUi949075.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75920; AAC75920; b2882.
BAE76948; BAE76948; BAE76948.
GeneIDi949075.
KEGGiecj:JW2850.
eco:b2882.
PATRICi32121174. VBIEscCol129921_2975.

Organism-specific databases

EchoBASEiEB2877.
EcoGeneiEG13065. xanQ.

Phylogenomic databases

eggNOGiENOG4105C2W. Bacteria.
COG2233. LUCA.
HOGENOMiHOG000038198.
InParanoidiP67444.
KOiK16346.
OMAiEGPAYLQ.
OrthoDBiEOG6M6JJK.
PhylomeDBiP67444.

Enzyme and pathway databases

BioCyciEcoCyc:YGFO-MONOMER.
ECOL316407:JW2850-MONOMER.
MetaCyc:YGFO-MONOMER.

Miscellaneous databases

PROiP67444.

Family and domain databases

InterProiIPR006042. Xan_ur_permease.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00801. ncs2. 1 hit.
PROSITEiPS01116. XANTH_URACIL_PERMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Cloning and functional characterization of two bacterial members of the NAT/NCS2 family in Escherichia coli."
    Karatza P., Frillingos S.
    Mol. Membr. Biol. 22:251-261(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
    Strain: K12.
  4. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Cysteine-scanning analysis of putative helix XII in the YgfO xanthine permease: ILE-432 and ASN-430 are important."
    Papakostas K., Georgopoulou E., Frillingos S.
    J. Biol. Chem. 283:13666-13678(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF PRO-421; SER-423; ILE-424; TYR-425; LEU-427; ASN-430; ILE-432 AND GLY-436.
    Strain: K12.
  6. "Role of intramembrane polar residues in the YgfO xanthine permease: HIS-31 and ASN-93 are crucial for affinity and specificity, and ASP-304 and GLU-272 are irreplaceable."
    Karena E., Frillingos S.
    J. Biol. Chem. 284:24257-24268(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF HIS-31; ASN-93; LYS-164; ASP-232; GLN-258; GLU-272; ASP-304 AND ARG-385.
    Strain: K12.

Entry informationi

Entry nameiXANQ_ECOLI
AccessioniPrimary (citable) accession number: P67444
Secondary accession number(s): Q2M9V8, Q46815
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: December 4, 2007
Last modified: May 11, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.