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Protein

Xanthine permease XanQ

Gene

xanQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specific, proton motive force-dependent high-affinity transporter for xanthine.1 Publication

Enzyme regulationi

Inhibited by CCCP and N-ethylmaleimide.1 Publication

Kineticsi

  1. KM=4.2 µM for xanthine1 Publication
  1. Vmax=6.36 nmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei31Essential for affinity and specificity1
Sitei93Essential for affinity and specificity1
Sitei272Essential for purine uptake1
Sitei304Essential for purine uptake1
Sitei430Important for purine uptake and affinity1
Sitei432Important for purine uptake and affinity1

GO - Molecular functioni

  • xanthine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • xanthine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:YGFO-MONOMER.
ECOL316407:JW2850-MONOMER.
MetaCyc:YGFO-MONOMER.

Protein family/group databases

TCDBi2.A.40.4.3. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Xanthine permease XanQ
Gene namesi
Name:xanQ
Synonyms:ygfO
Ordered Locus Names:b2882, JW2850
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13065. xanQ.

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 25CytoplasmicSequence analysisAdd BLAST25
Transmembranei26 – 46HelicalSequence analysisAdd BLAST21
Topological domaini47 – 55PeriplasmicSequence analysis9
Transmembranei56 – 76HelicalSequence analysisAdd BLAST21
Topological domaini77 – 80CytoplasmicSequence analysis4
Transmembranei81 – 101HelicalSequence analysisAdd BLAST21
Topological domaini102 – 120PeriplasmicSequence analysisAdd BLAST19
Transmembranei121 – 141HelicalSequence analysisAdd BLAST21
Topological domaini142 – 151CytoplasmicSequence analysis10
Transmembranei152 – 172HelicalSequence analysisAdd BLAST21
Topological domaini173 – 180PeriplasmicSequence analysis8
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Topological domaini202 – 210CytoplasmicSequence analysis9
Transmembranei211 – 231HelicalSequence analysisAdd BLAST21
Topological domaini232 – 258PeriplasmicSequence analysisAdd BLAST27
Transmembranei259 – 279HelicalSequence analysisAdd BLAST21
Topological domaini280 – 298CytoplasmicSequence analysisAdd BLAST19
Transmembranei299 – 319HelicalSequence analysisAdd BLAST21
Topological domaini320 – 342PeriplasmicSequence analysisAdd BLAST23
Transmembranei343 – 363HelicalSequence analysisAdd BLAST21
Topological domaini364CytoplasmicSequence analysis1
Transmembranei365 – 384HelicalSequence analysisAdd BLAST20
Topological domaini385 – 425PeriplasmicSequence analysisAdd BLAST41
Transmembranei426 – 446HelicalSequence analysisAdd BLAST21
Topological domaini447 – 466CytoplasmicSequence analysisAdd BLAST20

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31H → C or L: Decrease in activity. 1 Publication1
Mutagenesisi31H → K or R: Strong decrease in activity. 1 Publication1
Mutagenesisi31H → N: No change in activity. 1 Publication1
Mutagenesisi31H → Q: Increase of activity. 1 Publication1
Mutagenesisi93N → A: Highly active. 1 Publication1
Mutagenesisi93N → C, D or T: Strong decrease in activity. 1 Publication1
Mutagenesisi93N → Q: Loss of activity. 1 Publication1
Mutagenesisi93N → S: Increase of activity. 1 Publication1
Mutagenesisi164K → C or R: Highly active. 1 Publication1
Mutagenesisi232D → C: Decrease in activity. 1 Publication1
Mutagenesisi232D → E: Highly active. 1 Publication1
Mutagenesisi258Q → C: Decrease in activity. 1 Publication1
Mutagenesisi258Q → N: Highly active. 1 Publication1
Mutagenesisi272E → C: Loss of activity. 1 Publication1
Mutagenesisi272E → D: Strong decrease in activity. 1 Publication1
Mutagenesisi304D → C: Loss of activity. 1 Publication1
Mutagenesisi304D → E: Strong decrease in activity. 1 Publication1
Mutagenesisi385R → C: Decrease in activity. 1 Publication1
Mutagenesisi385R → K: Highly active. 1 Publication1
Mutagenesisi421P → C: Strong decrease in activity. 1 Publication1
Mutagenesisi421P → G: Highly active. 1 Publication1
Mutagenesisi423S → C: Highly active. 1 Publication1
Mutagenesisi424I → C: Highly active. 1 Publication1
Mutagenesisi425Y → C: Strong decrease in activity. 1 Publication1
Mutagenesisi427L → C: Decrease in activity. 1 Publication1
Mutagenesisi430N → T: Loss of activity. 1 Publication1
Mutagenesisi432I → A, S, T or V: Strong decrease in activity. 1 Publication1
Mutagenesisi432I → L, M, E or F: Loss of activity. 1 Publication1
Mutagenesisi432I → N or Q: Highly active. 1 Publication1
Mutagenesisi436G → C: Highly active. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001659651 – 466Xanthine permease XanQAdd BLAST466

Proteomic databases

PaxDbiP67444.
PRIDEiP67444.

Interactioni

Protein-protein interaction databases

BioGridi4261448. 20 interactors.
STRINGi511145.b2882.

Structurei

3D structure databases

ProteinModelPortaliP67444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C2W. Bacteria.
COG2233. LUCA.
HOGENOMiHOG000038198.
InParanoidiP67444.
KOiK16346.
OMAiEGPAYLQ.
PhylomeDBiP67444.

Family and domain databases

InterProiIPR006042. Xan_ur_permease.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00801. ncs2. 1 hit.
PROSITEiPS01116. XANTH_URACIL_PERMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P67444-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDINHAGSD LIFELEDRPP FHQALVGAIT HLLAIFVPMV TPALIVGAAL
60 70 80 90 100
QLSAETTAYL VSMAMIASGI GTWLQVNRYG IVGSGLLSIQ SVNFSFVTVM
110 120 130 140 150
IALGSSMKSD GFHEELIMSS LLGVSFVGAF LVVGSSFILP YLRRVITPTV
160 170 180 190 200
SGIVVLMIGL SLIKVGIIDF GGGFAAKSSG TFGNYEHLGV GLLVLIVVIG
210 220 230 240 250
FNCCRSPLLR MGGIAIGLCV GYIASLCLGM VDFSSMRNLP LITIPHPFKY
260 270 280 290 300
GFSFSFHQFL VVGTIYLLSV LEAVGDITAT AMVSRRPIQG EEYQSRLKGG
310 320 330 340 350
VLADGLVSVI ASAVGSLPLT TFAQNNGVIQ MTGVASRYVG RTIAVMLVIL
360 370 380 390 400
GLFPMIGGFF TTIPSAVLGG AMTLMFSMIA IAGIRIIITN GLKRRETLIV
410 420 430 440 450
ATSLGLGLGV SYDPEIFKIL PASIYVLVEN PICAGGLTAI LLNIILPGGY
460
RQENVLPGIT SAEEMD
Length:466
Mass (Da):49,108
Last modified:December 4, 2007 - v2
Checksum:i95EAFB06FEEE9175
GO

Sequence cautioni

The sequence AAA83063 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83063.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75920.2.
AP009048 Genomic DNA. Translation: BAE76948.1.
PIRiB65072.
RefSeqiNP_417358.2. NC_000913.3.
WP_001280192.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC75920; AAC75920; b2882.
BAE76948; BAE76948; BAE76948.
GeneIDi949075.
KEGGiecj:JW2850.
eco:b2882.
PATRICi32121174. VBIEscCol129921_2975.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28375 Genomic DNA. Translation: AAA83063.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75920.2.
AP009048 Genomic DNA. Translation: BAE76948.1.
PIRiB65072.
RefSeqiNP_417358.2. NC_000913.3.
WP_001280192.1. NZ_CP014272.1.

3D structure databases

ProteinModelPortaliP67444.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261448. 20 interactors.
STRINGi511145.b2882.

Protein family/group databases

TCDBi2.A.40.4.3. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Proteomic databases

PaxDbiP67444.
PRIDEiP67444.

Protocols and materials databases

DNASUi949075.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75920; AAC75920; b2882.
BAE76948; BAE76948; BAE76948.
GeneIDi949075.
KEGGiecj:JW2850.
eco:b2882.
PATRICi32121174. VBIEscCol129921_2975.

Organism-specific databases

EchoBASEiEB2877.
EcoGeneiEG13065. xanQ.

Phylogenomic databases

eggNOGiENOG4105C2W. Bacteria.
COG2233. LUCA.
HOGENOMiHOG000038198.
InParanoidiP67444.
KOiK16346.
OMAiEGPAYLQ.
PhylomeDBiP67444.

Enzyme and pathway databases

BioCyciEcoCyc:YGFO-MONOMER.
ECOL316407:JW2850-MONOMER.
MetaCyc:YGFO-MONOMER.

Miscellaneous databases

PROiP67444.

Family and domain databases

InterProiIPR006042. Xan_ur_permease.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00801. ncs2. 1 hit.
PROSITEiPS01116. XANTH_URACIL_PERMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiXANQ_ECOLI
AccessioniPrimary (citable) accession number: P67444
Secondary accession number(s): Q2M9V8, Q46815
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.