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Protein

HTH-type transcriptional repressor NemR

Gene

nemR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Represses the transcription of the nemRA operon by binding to the nemR box.1 Publication

Enzyme regulationi

NemR is inactivated by the addition of N-ethylmaleimide (NEM) and other Cys modification reagents.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi30 – 4920H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6889-MONOMER.
ECOL316407:JW5874-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional repressor NemR
Gene namesi
Name:nemR
Synonyms:ydhM
Ordered Locus Names:b1649, JW5874
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13947. nemR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 199199HTH-type transcriptional repressor NemRPRO_0000070639Add
BLAST

Proteomic databases

PaxDbiP67430.

Expressioni

Inductioni

Autoregulated.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
kdsBP049512EBI-544803,EBI-544810

Protein-protein interaction databases

BioGridi4260266. 2 interactions.
DIPiDIP-48165N.
IntActiP67430. 1 interaction.
STRINGi511145.b1649.

Structurei

Secondary structure

1
199
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 2417Combined sources
Turni26 – 283Combined sources
Helixi31 – 388Combined sources
Helixi42 – 487Combined sources
Helixi52 – 7726Combined sources
Beta strandi78 – 803Combined sources
Helixi82 – 9514Combined sources
Helixi107 – 1126Combined sources
Helixi113 – 1175Combined sources
Helixi120 – 14627Combined sources
Helixi156 – 17722Combined sources
Helixi181 – 19313Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4YZEX-ray2.20A/B/C/D1-199[»]
ProteinModelPortaliP67430.
SMRiP67430. Positions 8-194.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 6761HTH tetR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH tetR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108WUH. Bacteria.
ENOG4111IYW. LUCA.
HOGENOMiHOG000161259.
InParanoidiP67430.
KOiK16137.
OMAiWIDLHER.
PhylomeDBiP67430.

Family and domain databases

Gene3Di1.10.357.10. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
[Graphical view]
PfamiPF16925. TetR_C_13. 1 hit.
PF00440. TetR_N. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEiPS50977. HTH_TETR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P67430-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKHTEHDTR EHLLATGEQL CLQRGFTGMG LSELLKTAEV PKGSFYHYFR
60 70 80 90 100
SKEAFGVAML ERHYAAYHQR LTELLQSGEG NYRDRILAYY QQTLNQFCQH
110 120 130 140 150
GTISGCLTVK LSAEVCDLSE DMRSAMDKGA RGVIALLSQA LENGRENHCL
160 170 180 190
TFCGEPLQQA QVLYALWLGA NLQAKISRSF EPLENALAHV KNIIATPAV
Length:199
Mass (Da):22,275
Last modified:October 11, 2004 - v1
Checksum:iE01FDD7D575A1E09
GO

Sequence cautioni

The sequence BAE76492 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74721.3.
AP009048 Genomic DNA. Translation: BAE76492.1. Different initiation.
PIRiC64922.
RefSeqiNP_416166.3. NC_000913.3.
WP_001032936.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74721; AAC74721; b1649.
BAE76492; BAE76492; BAE76492.
GeneIDi946166.
KEGGiecj:JW5874.
eco:b1649.
PATRICi32118600. VBIEscCol129921_1720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74721.3.
AP009048 Genomic DNA. Translation: BAE76492.1. Different initiation.
PIRiC64922.
RefSeqiNP_416166.3. NC_000913.3.
WP_001032936.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4YZEX-ray2.20A/B/C/D1-199[»]
ProteinModelPortaliP67430.
SMRiP67430. Positions 8-194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260266. 2 interactions.
DIPiDIP-48165N.
IntActiP67430. 1 interaction.
STRINGi511145.b1649.

Proteomic databases

PaxDbiP67430.

Protocols and materials databases

DNASUi946166.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74721; AAC74721; b1649.
BAE76492; BAE76492; BAE76492.
GeneIDi946166.
KEGGiecj:JW5874.
eco:b1649.
PATRICi32118600. VBIEscCol129921_1720.

Organism-specific databases

EchoBASEiEB3705.
EcoGeneiEG13947. nemR.

Phylogenomic databases

eggNOGiENOG4108WUH. Bacteria.
ENOG4111IYW. LUCA.
HOGENOMiHOG000161259.
InParanoidiP67430.
KOiK16137.
OMAiWIDLHER.
PhylomeDBiP67430.

Enzyme and pathway databases

BioCyciEcoCyc:G6889-MONOMER.
ECOL316407:JW5874-MONOMER.

Miscellaneous databases

PROiP67430.

Family and domain databases

Gene3Di1.10.357.10. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
[Graphical view]
PfamiPF16925. TetR_C_13. 1 hit.
PF00440. TetR_N. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEiPS50977. HTH_TETR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEMR_ECOLI
AccessioniPrimary (citable) accession number: P67430
Secondary accession number(s): P76189, Q2MB64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.