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Protein

HTH-type transcriptional repressor NemR

Gene

nemR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Represses the transcription of the nemRA operon by binding to the nemR box.1 Publication

Enzyme regulationi

NemR is inactivated by the addition of N-ethylmaleimide (NEM) and other Cys modification reagents.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi30 – 49H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:G6889-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional repressor NemR
Gene namesi
Name:nemR
Synonyms:ydhM
Ordered Locus Names:b1649, JW5874
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13947 nemR

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000706391 – 199HTH-type transcriptional repressor NemRAdd BLAST199

Proteomic databases

PaxDbiP67430
PRIDEiP67430

Expressioni

Inductioni

Autoregulated.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
kdsBP049513EBI-544803,EBI-544810

Protein-protein interaction databases

BioGridi4260266, 2 interactors
DIPiDIP-48165N
IntActiP67430, 1 interactor
STRINGi316385.ECDH10B_1783

Structurei

Secondary structure

1199
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 24Combined sources17
Turni26 – 28Combined sources3
Helixi31 – 38Combined sources8
Helixi42 – 48Combined sources7
Helixi52 – 77Combined sources26
Beta strandi78 – 80Combined sources3
Helixi82 – 95Combined sources14
Helixi107 – 112Combined sources6
Helixi113 – 117Combined sources5
Helixi120 – 146Combined sources27
Helixi156 – 177Combined sources22
Helixi181 – 193Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YZEX-ray2.20A/B/C/D1-199[»]
ProteinModelPortaliP67430
SMRiP67430
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 67HTH tetR-typePROSITE-ProRule annotationAdd BLAST61

Phylogenomic databases

eggNOGiENOG4108WUH Bacteria
ENOG4111IYW LUCA
HOGENOMiHOG000161259
InParanoidiP67430
KOiK16137
OMAiTCLAVKL
PhylomeDBiP67430

Family and domain databases

InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR001647 HTH_TetR
IPR011075 Tet_transcr_reg_TetR-rel_C
IPR036271 Tet_transcr_reg_TetR-rel_C_sf
PfamiView protein in Pfam
PF16925 TetR_C_13, 1 hit
PF00440 TetR_N, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF48498 SSF48498, 1 hit
PROSITEiView protein in PROSITE
PS50977 HTH_TETR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P67430-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKHTEHDTR EHLLATGEQL CLQRGFTGMG LSELLKTAEV PKGSFYHYFR
60 70 80 90 100
SKEAFGVAML ERHYAAYHQR LTELLQSGEG NYRDRILAYY QQTLNQFCQH
110 120 130 140 150
GTISGCLTVK LSAEVCDLSE DMRSAMDKGA RGVIALLSQA LENGRENHCL
160 170 180 190
TFCGEPLQQA QVLYALWLGA NLQAKISRSF EPLENALAHV KNIIATPAV
Length:199
Mass (Da):22,275
Last modified:October 11, 2004 - v1
Checksum:iE01FDD7D575A1E09
GO

Sequence cautioni

The sequence BAE76492 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74721.3
AP009048 Genomic DNA Translation: BAE76492.1 Different initiation.
PIRiC64922
RefSeqiNP_416166.3, NC_000913.3
WP_001032936.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74721; AAC74721; b1649
BAE76492; BAE76492; BAE76492
GeneIDi946166
KEGGiecj:JW5874
eco:b1649
PATRICifig|1411691.4.peg.610

Similar proteinsi

Entry informationi

Entry nameiNEMR_ECOLI
AccessioniPrimary (citable) accession number: P67430
Secondary accession number(s): P76189, Q2MB64
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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