P67125 (Y1461_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UPF0051 protein Rv1461/MT1508

Cleaved into the following chain:

1. Endonuclease PI-MtuHIIP
   EC=3.1.-.-
   Alternative name(s):
   Mtu pps1 intein

Gene names

Ordered Locus Names:	Rv1461, MT1508
ORF Names:	MTV007.08

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	846 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Miscellaneous	Was identified as a high-confidence drug target.
Sequence similarities	Belongs to the UPF0051 (ycf24) family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: MTBBASE intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW iron-sulfur cluster assembly Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: MTBBASE
Molecular function	endonuclease activity Inferred from direct assay. Source: MTBBASE protein homodimerization activity Inferred from physical interaction. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	UPF0051 protein Rv1461/MT1508, 1st part Potential		PRO_0000036190
	Chain	253 – 611	359	Endonuclease PI-MtuHIIP Potential		PRO_0000036191
	Chain	612 – 846	235	UPF0051 protein Rv1461/MT1508, 2nd part Potential		PRO_0000036192
Regions
	Domain	388 – 528	141	DOD-type homing endonuclease

Sequences

	Sequence		Length	Mass (Da)
	P67125 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 468CEEF979B02222	FASTA	846	94,171
	10 20 30 40 50 60 MTLTPEASKS VAQPPTQAPL TQEEAIASLG RYGYGWADSD VAGANAQRGL SEAVVRDISA 70 80 90 100 110 120 KKNEPDWMLQ SRLKALRIFD RKPIPKWGSN LDGIDFDNIK YFVRSTEKQA ASWDDLPEDI 130 140 150 160 170 180 RNTYDRLGIP EAEKQRLVAG VAAQYESEVV YHQIREDLEA QGVIFLDTDT GLREHPDIFK 190 200 210 220 230 240 EYFGTVIPAG DNKFSALNTA VWSGGSFIYV PPGVHVDIPL QAYFRINTEN MGQFERTLII 250 260 270 280 290 300 ADEGSYVHYV EGCLPAGELI TTADGDLRPI ESIRVGDFVT GHDGRPHRVT AVQVRDLDGE 310 320 330 340 350 360 LFTFTPMSPA NAFSVTAEHP LLAIPRDEVR VMRKERNGWK AEVNSTKLRS AEPRWIAAKD 370 380 390 400 410 420 VAEGDFLIYP KPKPIPHRTV LPLEFARLAG YYLAEGHACL TNGCESLIFS FHSDEFEYVE 430 440 450 460 470 480 DVRQACKSLY EKSGSVLIEE HKHSARVTVY TKAGYAAMRD NVGIGSSNKK LSDLLMRQDE 490 500 510 520 530 540 TFLRELVDAY VNGDGNVTRR NGAVWKRVHT TSRLWAFQLQ SILARLGHYA TVELRRPGGP 550 560 570 580 590 600 GVIMGRNVVR KDIYQVQWTE GGRGPKQARD CGDYFAVPIK KRAVREAHEP VYNLDVENPD 610 620 630 640 650 660 SYLAYGFAVH NCTAPIYKSD SLHSAVVEII VKPHARVRYT TIQNWSNNVY NLVTKRARAE 670 680 690 700 710 720 AGATMEWIDG NIGSKVTMKY PAVWMTGEHA KGEVLSVAFA GEDQHQDTGA KMLHLAPNTS 730 740 750 760 770 780 SNIVSKSVAR GGGRTSYRGL VQVNKGAHGS RSSVKCDALL VDTVSRSDTY PYVDIREDDV 790 800 810 820 830 840 TMGHEATVSK VSENQLFYLM SRGLTEDEAM AMVVRGFVEP IAKELPMEYA LELNRLIELQ MEGAVG

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842576 Genomic DNA. Translation: CAA15991.1. AE000516 Genomic DNA. Translation: AAK45772.1.
PIR	H70871.
RefSeq	NP_215977.1. NC_000962.2. NP_335958.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P67125.
ModBase	Search...
Protein family/group databases
REBASE	4231. PI-MtuHIIP.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000000183; EBMYCP00000000183; EBMYCG00000000183. EBMYCT00000072074; EBMYCP00000070133; EBMYCG00000072069.
GeneID	886609. 924461.
GenomeReviews	Gene locus MT1508 in contig AE000516_GR. Gene locus Rv1461 in contig AL123456_GR.
KEGG	mtc:MT1508. mtu:Rv1461.
PATRIC	18125082. VBIMycTub22151_1656.
TIGR	MT1508.
Organism-specific databases
TubercuList	Rv1461.
Phylogenomic databases
GeneTree	EBGT00050000017319.
HOGENOM	HBG130984.
PhylomeDB	P67125.
ProtClustDB	CLSK791176.
Family and domain databases
InterPro	IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR007868. Hom_end_hint. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR010231. SUF_FeS_clus_asmbl_SufB. IPR000825. SUF_FeS_clus_asmbl_SufBD. [Graphical view]
KO	K09014.
Pfam	PF05203. Hom_end_hint. 1 hit. PF01458. UPF0051. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. Intein_Cterm. 1 hit. TIGR01980. SufB. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y1461_MYCTU

Accession

Primary (citable) accession number: P67125
Secondary accession number(s): O53152

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program