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Protein

Phosphodiesterase YfcE

Gene

yfcE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Shows phosphodiesterase activity, hydrolyzing phosphodiesters bonds in the artificial chromogenic substrates bis-p-nitrophenyl phosphate (bis-pNPP), and less efficiently thymidine 5'-monophosphate p-nitrophenyl ester (pNP-TMP) and p-nitrophenylphosphorylcholine (pNPPC). The physiological substrate is unknown.1 Publication

Cofactori

Mn2+1 PublicationNote: Binds 2 manganese ions per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9Manganese 11
Metal bindingi11Manganese 11
Metal bindingi37Manganese 11
Metal bindingi37Manganese 21
Metal bindingi73Manganese 21
Metal bindingi105Manganese 21
Metal bindingi127Manganese 21
Metal bindingi129Manganese 11

GO - Molecular functioni

  • hydrolase activity Source: UniProtKB-KW
  • zinc ion binding Source: EcoCyc

Keywordsi

Molecular functionHydrolase
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7192-MONOMER
BRENDAi3.1.4.1 2026
3.1.4.16 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphodiesterase YfcE (EC:3.1.4.-)
Gene namesi
Name:yfcE
Ordered Locus Names:b2300, JW5377
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14108 yfcE

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001556051 – 184Phosphodiesterase YfcEAdd BLAST184

Proteomic databases

PaxDbiP67095
PRIDEiP67095

Interactioni

Protein-protein interaction databases

BioGridi4260515, 361 interactors
STRINGi316385.ECDH10B_2462

Structurei

Secondary structure

1184
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi14 – 27Combined sources14
Beta strandi30 – 34Combined sources5
Helixi53 – 61Combined sources9
Helixi62 – 66Combined sources5
Beta strandi67 – 69Combined sources3
Helixi77 – 82Combined sources6
Beta strandi83 – 85Combined sources3
Beta strandi90 – 95Combined sources6
Beta strandi100 – 104Combined sources5
Beta strandi106 – 110Combined sources5
Beta strandi122 – 124Combined sources3
Beta strandi132 – 136Combined sources5
Beta strandi139 – 143Combined sources5
Beta strandi157 – 162Combined sources6
Beta strandi165 – 170Combined sources6
Turni171 – 173Combined sources3
Beta strandi176 – 181Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SU1X-ray2.25A/B/C/D1-184[»]
ProteinModelPortaliP67095
SMRiP67095
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP67095

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108RE9 Bacteria
COG0622 LUCA
HOGENOMiHOG000293432
InParanoidiP67095
KOiK07095
OMAiVAVRGNC
PhylomeDBiP67095

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR024654 Calcineurin-like_PHP_lpxH
IPR029052 Metallo-depent_PP-like
IPR020935 PdiEstase_YfcE_CS
IPR000979 Phosphodiesterase_MJ0936/Vps29
PANTHERiPTHR11124 PTHR11124, 1 hit
PfamiView protein in Pfam
PF12850 Metallophos_2, 1 hit
TIGRFAMsiTIGR00040 yfcE, 1 hit
PROSITEiView protein in PROSITE
PS01269 UPF0025, 1 hit

Sequencei

Sequence statusi: Complete.

P67095-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKLMFASDI HGSLPATERV LELFAQSGAQ WLVILGDVLN HGPRNALPEG
60 70 80 90 100
YAPAKVAERL NEVAHKVIAV RGNCDSEVDQ MLLHFPITAP WQQVLLEKQR
110 120 130 140 150
LFLTHGHLFG PENLPALNQN DVLVYGHTHL PVAEQRGEIF HFNPGSVSIP
160 170 180
KGGNPASYGM LDNDVLSVIA LNDQSIIAQV AINP
Length:184
Mass (Da):20,122
Last modified:October 11, 2004 - v1
Checksum:i15C5AA0BE6541AB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75360.1
AP009048 Genomic DNA Translation: BAE76687.1
PIRiB65002
RefSeqiNP_416803.3, NC_000913.3
WP_000977007.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75360; AAC75360; b2300
BAE76687; BAE76687; BAE76687
GeneIDi946755
KEGGiecj:JW5377
eco:b2300
PATRICifig|511145.12.peg.2395

Similar proteinsi

Entry informationi

Entry nameiYFCE_ECOLI
AccessioniPrimary (citable) accession number: P67095
Secondary accession number(s): P76495, Q2MAL9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
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Main funding by: National Institutes of Health