P67088 (RSMI_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase I
EC=2.1.1.198

Alternative name(s):
16S rRNA 2'-O-ribose C1402 methyltransferase
rRNA (cytidine-2'-O-)-methyltransferase RsmI

Gene names

Name:	rsmI
Ordered Locus Names:	Z4505, ECs4027

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA By similarity. HAMAP MF_01877
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytosine(1402) in 16S rRNA. HAMAP MF_01877
Subcellular location	Cytoplasm Potential HAMAP MF_01877.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmI family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Ribosomal RNA small subunit methyltransferase I HAMAP MF_01877		PRO_0000211939

Sequences

	Sequence		Length	Mass (Da)
	P67088 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 886EA492858FB95C	FASTA	286	31,348
	10 20 30 40 50 60 MKQHQSADNS QGQLYIVPTP IGNLADITQR ALEVLQAVDL IAAEDTRHTG LLLQHFGINA 70 80 90 100 110 120 RLFALHDHNE QQKAETLLAK LQEGQNIALV SDAGTPLIND PGYHLVRTCR EAGIRVVPLP 130 140 150 160 170 180 GPCAAITALS AAGLPSDRFC YEGFLPAKSK GRRDALKAIE AEPRTLIFYE STHRLLDSLE 190 200 210 220 230 240 DIVAVLGESR YVVLARELTK TWETIHGAPV GELLAWVKED ENRRKGEMVL IVEGHKAQEE 250 260 270 280 DLPADALRTL ALLQAELPLK KAAALAAEIH GVKKNALYKY ALEQQG

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG58282.1. BA000007 Genomic DNA. Translation: BAB37450.1.
PIR	C91132. F85977.
RefSeq	NP_289722.1. NC_002655.2. NP_312054.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P67088.
SMR	P67088. Positions 10-237.
ModBase	Search...
Proteomic databases
PRIDE	P67088.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000026925; EBESCP00000025818; EBESCG00000025978. EBESCT00000059148; EBESCP00000056976; EBESCG00000058196.
GeneID	916135. 958599.
GenomeReviews	Gene locus Z4505 in contig AE005174_GR. Gene locus ECs4027 in contig BA000007_GR.
KEGG	ece:Z4505. ecs:ECs4027.
PATRIC	18357570. VBIEscCol44059_3961.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000009537.
HOGENOM	HBG529058.
OMA	IASDRFC.
ProtClustDB	PRK14994.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS4027-MONOMER.
Family and domain databases
HAMAP	MF_01877. 16SrRNA_methyltr_I. [Tree]
InterPro	IPR000878. 4pyrrol_Mease. IPR014777. 4pyrrole_Mease_sub1. IPR008189. rRNA_ssu_MeTfrase_I. IPR018063. SAM_MeTrfase_RsmI_CS. [Graphical view]
Gene3D	G3DSA:3.40.1010.10. 4pyrrole_Mease_sub1. 1 hit.
KO	K07056.
PANTHER	PTHR21091:SF18. PTHR21091:SF18. 1 hit.
Pfam	PF00590. TP_methylase. 1 hit. [Graphical view]
PIRSF	PIRSF005917. MTase_YraL. 1 hit.
SUPFAM	SSF53790. Cor/por_Metransf. 1 hit.
TIGRFAMs	TIGR00096. TIGR00096. 1 hit.
PROSITE	PS01296. RSMI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSMI_ECO57

Accession

Primary (citable) accession number: P67088
Secondary accession number(s): P45528

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program