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Protein

Ribosomal RNA small subunit methyltransferase I

Gene

rsmI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA. In vitro, active on the assembled 30S subunit, but not naked 16S rRNA or 70S ribosomes.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + cytidine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(1402) in 16S rRNA.1 Publication

GO - Molecular functioni

  • rRNA (cytosine-2'-O-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • enzyme-directed rRNA 2'-O-methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G7641-MONOMER.
ECOL316407:JW3115-MONOMER.
MetaCyc:G7641-MONOMER.
BRENDAi2.1.1.198. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase I (EC:2.1.1.198)
Alternative name(s):
16S rRNA 2'-O-ribose C1402 methyltransferase
rRNA (cytidine-2'-O-)-methyltransferase RsmI
Gene namesi
Name:rsmI
Synonyms:yraL
Ordered Locus Names:b3146, JW3115
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12777. rsmI.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286Ribosomal RNA small subunit methyltransferase IPRO_0000211938Add
BLAST

Proteomic databases

EPDiP67087.
PaxDbiP67087.
PRIDEiP67087.

Interactioni

Protein-protein interaction databases

BioGridi4261593. 49 interactions.
DIPiDIP-12896N.
IntActiP67087. 3 interactions.
MINTiMINT-1312386.
STRINGi511145.b3146.

Structurei

3D structure databases

ProteinModelPortaliP67087.
SMRiP67087. Positions 12-237.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CU0. Bacteria.
COG0313. LUCA.
HOGENOMiHOG000195941.
InParanoidiP67087.
KOiK07056.
OMAiCEDTRNT.
PhylomeDBiP67087.

Family and domain databases

CDDicd11648. RsmI. 1 hit.
Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
HAMAPiMF_01877. 16SrRNA_methyltr_I. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR008189. rRNA_ssu_MeTfrase_I.
IPR018063. SAM_MeTrfase_RsmI_CS.
[Graphical view]
PANTHERiPTHR21091:SF18. PTHR21091:SF18. 1 hit.
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF005917. MTase_YraL. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR00096. TIGR00096. 1 hit.
PROSITEiPS01296. RSMI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P67087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQHQSADNS QGQLYIVPTP IGNLADITQR ALEVLQAVDL IAAEDTRHTG
60 70 80 90 100
LLLQHFGINA RLFALHDHNE QQKAETLLAK LQEGQNIALV SDAGTPLIND
110 120 130 140 150
PGYHLVRTCR EAGIRVVPLP GPCAAITALS AAGLPSDRFC YEGFLPAKSK
160 170 180 190 200
GRRDALKAIE AEPRTLIFYE STHRLLDSLE DIVAVLGESR YVVLARELTK
210 220 230 240 250
TWETIHGAPV GELLAWVKED ENRRKGEMVL IVEGHKAQEE DLPADALRTL
260 270 280
ALLQAELPLK KAAALAAEIH GVKKNALYKY ALEQQG
Length:286
Mass (Da):31,348
Last modified:October 11, 2004 - v1
Checksum:i886EA492858FB95C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57949.1.
U00096 Genomic DNA. Translation: AAC76180.1.
AP009048 Genomic DNA. Translation: BAE77192.1.
PIRiF65104.
RefSeqiNP_417615.1. NC_000913.3.
WP_000809262.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76180; AAC76180; b3146.
BAE77192; BAE77192; BAE77192.
GeneIDi947664.
KEGGiecj:JW3115.
eco:b3146.
PATRICi32121710. VBIEscCol129921_3241.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57949.1.
U00096 Genomic DNA. Translation: AAC76180.1.
AP009048 Genomic DNA. Translation: BAE77192.1.
PIRiF65104.
RefSeqiNP_417615.1. NC_000913.3.
WP_000809262.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP67087.
SMRiP67087. Positions 12-237.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261593. 49 interactions.
DIPiDIP-12896N.
IntActiP67087. 3 interactions.
MINTiMINT-1312386.
STRINGi511145.b3146.

Proteomic databases

EPDiP67087.
PaxDbiP67087.
PRIDEiP67087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76180; AAC76180; b3146.
BAE77192; BAE77192; BAE77192.
GeneIDi947664.
KEGGiecj:JW3115.
eco:b3146.
PATRICi32121710. VBIEscCol129921_3241.

Organism-specific databases

EchoBASEiEB2630.
EcoGeneiEG12777. rsmI.

Phylogenomic databases

eggNOGiENOG4105CU0. Bacteria.
COG0313. LUCA.
HOGENOMiHOG000195941.
InParanoidiP67087.
KOiK07056.
OMAiCEDTRNT.
PhylomeDBiP67087.

Enzyme and pathway databases

BioCyciEcoCyc:G7641-MONOMER.
ECOL316407:JW3115-MONOMER.
MetaCyc:G7641-MONOMER.
BRENDAi2.1.1.198. 2026.

Miscellaneous databases

PROiP67087.

Family and domain databases

CDDicd11648. RsmI. 1 hit.
Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
HAMAPiMF_01877. 16SrRNA_methyltr_I. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR008189. rRNA_ssu_MeTfrase_I.
IPR018063. SAM_MeTrfase_RsmI_CS.
[Graphical view]
PANTHERiPTHR21091:SF18. PTHR21091:SF18. 1 hit.
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF005917. MTase_YraL. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR00096. TIGR00096. 1 hit.
PROSITEiPS01296. RSMI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSMI_ECOLI
AccessioniPrimary (citable) accession number: P67087
Secondary accession number(s): P45528, Q2M964
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.