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Protein

Ribosomal RNA small subunit methyltransferase I

Gene

rsmI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA. In vitro, active on the assembled 30S subunit, but not naked 16S rRNA or 70S ribosomes.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + cytidine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(1402) in 16S rRNA.1 Publication

GO - Molecular functioni

  • rRNA (cytosine-2'-O-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • enzyme-directed rRNA 2'-O-methylation Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G7641-MONOMER.
MetaCyc:G7641-MONOMER.
BRENDAi2.1.1.198. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase I (EC:2.1.1.198)
Alternative name(s):
16S rRNA 2'-O-ribose C1402 methyltransferase
rRNA (cytidine-2'-O-)-methyltransferase RsmI
Gene namesi
Name:rsmI
Synonyms:yraL
Ordered Locus Names:b3146, JW3115
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12777. rsmI.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002119381 – 286Ribosomal RNA small subunit methyltransferase IAdd BLAST286

Proteomic databases

EPDiP67087.
PaxDbiP67087.
PRIDEiP67087.

Interactioni

Protein-protein interaction databases

BioGridi4261593. 63 interactors.
DIPiDIP-12896N.
IntActiP67087. 3 interactors.
MINTiMINT-1312386.
STRINGi511145.b3146.

Structurei

Secondary structure

1286
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 16Combined sources3
Helixi24 – 26Combined sources3
Helixi29 – 37Combined sources9
Beta strandi38 – 44Combined sources7
Helixi46 – 55Combined sources10
Beta strandi62 – 64Combined sources3
Helixi71 – 82Combined sources12
Beta strandi87 – 90Combined sources4
Beta strandi92 – 94Combined sources3
Beta strandi98 – 101Combined sources4
Helixi103 – 110Combined sources8
Turni111 – 113Combined sources3
Beta strandi116 – 118Combined sources3
Helixi124 – 131Combined sources8
Beta strandi137 – 144Combined sources8
Helixi149 – 158Combined sources10
Turni159 – 161Combined sources3
Beta strandi164 – 170Combined sources7
Turni172 – 174Combined sources3
Helixi175 – 186Combined sources12
Beta strandi190 – 197Combined sources8
Beta strandi204 – 209Combined sources6
Helixi210 – 219Combined sources10
Helixi221 – 224Combined sources4
Beta strandi228 – 232Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5HW4X-ray2.21A/B/C12-258[»]
ProteinModelPortaliP67087.
SMRiP67087.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CU0. Bacteria.
COG0313. LUCA.
HOGENOMiHOG000195941.
InParanoidiP67087.
KOiK07056.
OMAiCEDTRNT.
PhylomeDBiP67087.

Family and domain databases

CDDicd11648. RsmI. 1 hit.
Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
HAMAPiMF_01877. 16SrRNA_methyltr_I. 1 hit.
InterProiView protein in InterPro
IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR008189. rRNA_ssu_MeTfrase_I.
IPR018063. SAM_MeTrfase_RsmI_CS.
PANTHERiPTHR21091:SF132. PTHR21091:SF132. 1 hit.
PfamiView protein in Pfam
PF00590. TP_methylase. 1 hit.
PIRSFiPIRSF005917. MTase_YraL. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR00096. TIGR00096. 1 hit.
PROSITEiView protein in PROSITE
PS01296. RSMI. 1 hit.

Sequencei

Sequence statusi: Complete.

P67087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQHQSADNS QGQLYIVPTP IGNLADITQR ALEVLQAVDL IAAEDTRHTG
60 70 80 90 100
LLLQHFGINA RLFALHDHNE QQKAETLLAK LQEGQNIALV SDAGTPLIND
110 120 130 140 150
PGYHLVRTCR EAGIRVVPLP GPCAAITALS AAGLPSDRFC YEGFLPAKSK
160 170 180 190 200
GRRDALKAIE AEPRTLIFYE STHRLLDSLE DIVAVLGESR YVVLARELTK
210 220 230 240 250
TWETIHGAPV GELLAWVKED ENRRKGEMVL IVEGHKAQEE DLPADALRTL
260 270 280
ALLQAELPLK KAAALAAEIH GVKKNALYKY ALEQQG
Length:286
Mass (Da):31,348
Last modified:October 11, 2004 - v1
Checksum:i886EA492858FB95C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57949.1.
U00096 Genomic DNA. Translation: AAC76180.1.
AP009048 Genomic DNA. Translation: BAE77192.1.
PIRiF65104.
RefSeqiNP_417615.1. NC_000913.3.
WP_000809262.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76180; AAC76180; b3146.
BAE77192; BAE77192; BAE77192.
GeneIDi947664.
KEGGiecj:JW3115.
eco:b3146.
PATRICi32121710. VBIEscCol129921_3241.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57949.1.
U00096 Genomic DNA. Translation: AAC76180.1.
AP009048 Genomic DNA. Translation: BAE77192.1.
PIRiF65104.
RefSeqiNP_417615.1. NC_000913.3.
WP_000809262.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5HW4X-ray2.21A/B/C12-258[»]
ProteinModelPortaliP67087.
SMRiP67087.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261593. 63 interactors.
DIPiDIP-12896N.
IntActiP67087. 3 interactors.
MINTiMINT-1312386.
STRINGi511145.b3146.

Proteomic databases

EPDiP67087.
PaxDbiP67087.
PRIDEiP67087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76180; AAC76180; b3146.
BAE77192; BAE77192; BAE77192.
GeneIDi947664.
KEGGiecj:JW3115.
eco:b3146.
PATRICi32121710. VBIEscCol129921_3241.

Organism-specific databases

EchoBASEiEB2630.
EcoGeneiEG12777. rsmI.

Phylogenomic databases

eggNOGiENOG4105CU0. Bacteria.
COG0313. LUCA.
HOGENOMiHOG000195941.
InParanoidiP67087.
KOiK07056.
OMAiCEDTRNT.
PhylomeDBiP67087.

Enzyme and pathway databases

BioCyciEcoCyc:G7641-MONOMER.
MetaCyc:G7641-MONOMER.
BRENDAi2.1.1.198. 2026.

Miscellaneous databases

PROiP67087.

Family and domain databases

CDDicd11648. RsmI. 1 hit.
Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
HAMAPiMF_01877. 16SrRNA_methyltr_I. 1 hit.
InterProiView protein in InterPro
IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR008189. rRNA_ssu_MeTfrase_I.
IPR018063. SAM_MeTrfase_RsmI_CS.
PANTHERiPTHR21091:SF132. PTHR21091:SF132. 1 hit.
PfamiView protein in Pfam
PF00590. TP_methylase. 1 hit.
PIRSFiPIRSF005917. MTase_YraL. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR00096. TIGR00096. 1 hit.
PROSITEiView protein in PROSITE
PS01296. RSMI. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRSMI_ECOLI
AccessioniPrimary (citable) accession number: P67087
Secondary accession number(s): P45528, Q2M964
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 15, 2017
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.