P67054 (COXX_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protoheme IX farnesyltransferase
EC=2.5.1.-

Alternative name(s):
Heme B farnesyltransferase
Heme O synthase

Gene names

Name:	ctaB
Ordered Locus Names:	BR0469, BS1330_I0470

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group By similarity. HAMAP MF_00154
Pathway	Porphyrin metabolism; heme O biosynthesis; heme O from protoheme: step 1/1. HAMAP MF_00154
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential HAMAP MF_00154.
Miscellaneous	Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature By similarity. HAMAP MF_00154
Sequence similarities	Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.
Sequence caution	The sequence AAN29412.1 differs from that shown. Reason: Erroneous initiation. The sequence AEM17825.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Heme biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme O biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protoheme IX farnesyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Protoheme IX farnesyltransferase HAMAP MF_00154		PRO_0000162907
Regions
	Transmembrane	29 – 49	21	Helical; Potential
	Transmembrane	50 – 70	21	Helical; Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Transmembrane	117 – 137	21	Helical; Potential
	Transmembrane	150 – 170	21	Helical; Potential
	Transmembrane	177 – 197	21	Helical; Potential
	Transmembrane	223 – 243	21	Helical; Potential
	Transmembrane	246 – 266	21	Helical; Potential
	Transmembrane	292 – 312	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P67054 [UniParc]. Last modified April 8, 2008. Version 2. Checksum: 36803FD3F9BA2B49	FASTA	312	34,106
	10 20 30 40 50 60 MEKNTASEDA FALSEATARD YLVLLKPRVM SLVVFTGLVG LVLAPGHMNP VLAVISILCI 70 80 90 100 110 120 AVGAGASGAL NMWYDADIDA VMKRTRKRPI PAGIIAPNQV LAFGLTLSAF SVMTLGLMVN 130 140 150 160 170 180 WLAAALLAFT IFFYAVIYTM WLKRSTPQNI VIGGAAGAFP PMIGWAAATG EITWDSLVLF 190 200 210 220 230 240 MIIFLWTPPH FWALSLFTTN DYEAARIPMM PNVKGELSTR RQALFYAVLM APVGVLPWVM 250 260 270 280 290 300 GFAGMFYGVV STLLGLAFVY YAWRLWAADS QPQMLAAARK LFRFSLLYLA GIFAVLLFEA 310 LTFKLLAAFG VF

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29412.1. Different initiation. CP002997 Genomic DNA. Translation: AEM17825.1. Different initiation.
RefSeq	NP_697497.1. NC_004310.3.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1166130.
GenomeReviews	Gene locus BR0469 in contig AE014291_GR.
KEGG	bms:BR0469.
PATRIC	17789232. VBIBruSui107850_0484.
TIGR	BR0469.
Phylogenomic databases
HOGENOM	HBG656003.
ProtClustDB	PRK04375.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0469-MONOMER.
Family and domain databases
HAMAP	MF_00154. CyoE_CtaB. [Tree]
InterPro	IPR006369. Protohaem_IX_farnesylTrfase. IPR000537. UbiA_prenyltransferase. [Graphical view]
KO	K02301.
Pfam	PF01040. UbiA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01473. CyoE_ctaB. 1 hit.
PROSITE	PS00943. UBIA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COXX_BRUSU

Accession

Primary (citable) accession number: P67054
Secondary accession number(s): G0K718, Q8YFQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program