Reviewed, UniProtKB/Swiss-Prot P67054 (COXX_BRUSU)

Names and origin

Protein names

Recommended name:
    Protoheme IX farnesyltransferase
    EC=2.5.1.-
Alternative name(s):
    Heme O synthase
    Heme B farnesyltransferase

Gene names

Name:	ctaB
Ordered Locus Names:	BR0469

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group By similarity.
Pathway	Porphyrin metabolism; heme O biosynthesis; heme O from protoheme: step 1/1. HAMAP MF_00154
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Miscellaneous	Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature By similarity.
Sequence similarities	Belongs to the ubiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.

Ontologies

Keywords
Biological process	Heme biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme O biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	protoheme IX farnesyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Protoheme IX farnesyltransferase HAMAP MF_00154		PRO_0000162907
Regions
	Transmembrane	29 – 49	21	Potential
	Transmembrane	50 – 70	21	Potential
	Transmembrane	90 – 110	21	Potential
	Transmembrane	117 – 137	21	Potential
	Transmembrane	150 – 170	21	Potential
	Transmembrane	177 – 197	21	Potential
	Transmembrane	223 – 243	21	Potential
	Transmembrane	246 – 266	21	Potential
	Transmembrane	292 – 312	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P67054-1 [UniParc]. Last modified April 8, 2008. Version 2. Checksum: 36803FD3F9BA2B49	FASTA	312	34,106
	10 20 30 40 50 60 MEKNTASEDA FALSEATARD YLVLLKPRVM SLVVFTGLVG LVLAPGHMNP VLAVISILCI 70 80 90 100 110 120 AVGAGASGAL NMWYDADIDA VMKRTRKRPI PAGIIAPNQV LAFGLTLSAF SVMTLGLMVN 130 140 150 160 170 180 WLAAALLAFT IFFYAVIYTM WLKRSTPQNI VIGGAAGAFP PMIGWAAATG EITWDSLVLF 190 200 210 220 230 240 MIIFLWTPPH FWALSLFTTN DYEAARIPMM PNVKGELSTR RQALFYAVLM APVGVLPWVM 250 260 270 280 290 300 GFAGMFYGVV STLLGLAFVY YAWRLWAADS QPQMLAAARK LFRFSLLYLA GIFAVLLFEA 310 LTFKLLAAFG VF

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014291 Genomic DNA. Translation: AAN29412.1. Different initiation.
RefSeq	NP_697497.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1166130.
GenomeReviews	Gene locus BR0469 in contig AE014291_GR.
KEGG	bms:BR0469.
TIGR	BR0469.
Phylogenomic databases
HOGENOM	P67054.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0469-MON.
Family and domain databases
HAMAP	MF_00154. [Tree]
InterPro	IPR006369. Protohaem_IX_farnesylTrfase. IPR000537. UbiA_prenyltransferase. [Graphical view]
PANTHER	PTHR11048:SF3. CyoE_CtaB. 1 hit.
Pfam	PF01040. UbiA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01473. cyoE_ctaB. 1 hit.
PROSITE	PS00943. UBIA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COXX_BRUSU

Accession

Primary (citable) accession number: P67054
Secondary accession number(s): Q8YFQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	April 8, 2008
Last modified:	October 13, 2009
This is version 29 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)