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P67046

- TYSY_STAAM

UniProt

P67046 - TYSY_STAAM

Protein

Thymidylate synthase

Gene

thyA

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    • Comment

    Functioni

    Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei201 – 2011UniRule annotation

    GO - Molecular functioni

    1. thymidylate synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. dTMP biosynthetic process Source: UniProtKB-HAMAP
    2. dTTP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Enzyme and pathway databases

    BioCyciSAUR158878:GJJ5-1443-MONOMER.
    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
    Short name:
    TSUniRule annotation
    Short name:
    TSaseUniRule annotation
    Gene namesi
    Name:thyAUniRule annotation
    Ordered Locus Names:SAV1427
    OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
    Taxonomic identifieri158878 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000002481: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 318318Thymidylate synthasePRO_0000141018Add
    BLAST

    2D gel databases

    World-2DPAGE0002:P67046.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi158878.SAV1427.

    Structurei

    Secondary structure

    1
    318
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1916
    Beta strandi21 – 233
    Beta strandi31 – 4212
    Helixi43 – 453
    Beta strandi51 – 533
    Helixi57 – 6913
    Helixi74 – 785
    Turni79 – 813
    Helixi86 – 938
    Helixi109 – 1135
    Helixi117 – 13418
    Helixi136 – 1427
    Helixi149 – 1546
    Helixi166 – 17611
    Beta strandi184 – 1863
    Helixi190 – 1923
    Turni193 – 1953
    Beta strandi196 – 1983
    Beta strandi201 – 21010
    Beta strandi213 – 22412
    Turni225 – 2273
    Helixi228 – 24619
    Beta strandi250 – 26415
    Helixi268 – 2769
    Beta strandi284 – 2874
    Helixi293 – 2953
    Helixi298 – 3003
    Beta strandi301 – 3055

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4DQ1X-ray2.71A/B1-318[»]
    ProteinModelPortaliP67046.
    SMRiP67046. Positions 3-314.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0207.
    KOiK00560.
    OMAiDVHLYSN.
    OrthoDBiEOG6K6V53.
    PhylomeDBiP67046.

    Family and domain databases

    Gene3Di3.30.572.10. 2 hits.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 1 hit.
    PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P67046-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLNSFDAAYH SLCEEVLEIG NTRNDRTNTG TISKFGHQLR FDLSKGFPLL    50
    TTKKVSFKLV ATELLWFIKG DTNIQYLLKY NNNIWNEWAF ENYIKSDEYN 100
    GPDMTDFGHR ALSDPEFNEQ YKEQMKQFKQ RILEDDTFAK QFGDLGNVYG 150
    KQWRDWVDKD GNHFDQLKTV IEQIKHNPDS RRHIVSAWNP TEIDTMALPP 200
    CHTMFQFYVQ DGKLSCQLYQ RSADIFLGVP FNIASYALLT HLIAKECGLE 250
    VGEFVHTFGD AHIYSNHIDA IQTQLARESF NPPTLKINSD KSIFDINYED 300
    LEIVDYESHP AIKAPIAV 318
    Length:318
    Mass (Da):36,825
    Last modified:October 11, 2004 - v1
    Checksum:iE7F41BA915C25D37
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000017 Genomic DNA. Translation: BAB57589.1.
    RefSeqiNP_371951.1. NC_002758.2.

    Genome annotation databases

    EnsemblBacteriaiBAB57589; BAB57589; SAV1427.
    GeneIDi1121402.
    KEGGisav:SAV1427.
    PATRICi19563578. VBIStaAur52173_1466.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000017 Genomic DNA. Translation: BAB57589.1 .
    RefSeqi NP_371951.1. NC_002758.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4DQ1 X-ray 2.71 A/B 1-318 [» ]
    ProteinModelPortali P67046.
    SMRi P67046. Positions 3-314.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 158878.SAV1427.

    2D gel databases

    World-2DPAGE 0002:P67046.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB57589 ; BAB57589 ; SAV1427 .
    GeneIDi 1121402.
    KEGGi sav:SAV1427.
    PATRICi 19563578. VBIStaAur52173_1466.

    Phylogenomic databases

    eggNOGi COG0207.
    KOi K00560.
    OMAi DVHLYSN.
    OrthoDBi EOG6K6V53.
    PhylomeDBi P67046.

    Enzyme and pathway databases

    UniPathwayi UPA00575 .
    BioCyci SAUR158878:GJJ5-1443-MONOMER.

    Family and domain databases

    Gene3Di 3.30.572.10. 2 hits.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 1 hit.
    PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Mu50 / ATCC 700699.

    Entry informationi

    Entry nameiTYSY_STAAM
    AccessioniPrimary (citable) accession number: P67046
    Secondary accession number(s): Q99U61
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3