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Protein

Thymidylate synthase

Gene

thyA

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathway: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei201 – 2011UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-1443-MONOMER.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:SAV1427
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Thymidylate synthasePRO_0000141018Add
BLAST

2D gel databases

World-2DPAGE0002:P67046.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi158878.SAV1427.

Structurei

Secondary structure

1
318
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1916Combined sources
Beta strandi21 – 233Combined sources
Beta strandi31 – 4212Combined sources
Helixi43 – 453Combined sources
Beta strandi51 – 533Combined sources
Helixi57 – 6913Combined sources
Helixi74 – 785Combined sources
Turni79 – 813Combined sources
Helixi86 – 938Combined sources
Helixi109 – 1135Combined sources
Helixi117 – 13418Combined sources
Helixi136 – 1427Combined sources
Helixi149 – 1546Combined sources
Helixi166 – 17611Combined sources
Beta strandi184 – 1863Combined sources
Helixi190 – 1923Combined sources
Turni193 – 1953Combined sources
Beta strandi196 – 1983Combined sources
Beta strandi201 – 21010Combined sources
Beta strandi213 – 22412Combined sources
Turni225 – 2273Combined sources
Helixi228 – 24619Combined sources
Beta strandi250 – 26415Combined sources
Helixi268 – 2769Combined sources
Beta strandi284 – 2874Combined sources
Helixi293 – 2953Combined sources
Helixi298 – 3003Combined sources
Beta strandi301 – 3055Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DQ1X-ray2.71A/B1-318[»]
ProteinModelPortaliP67046.
SMRiP67046. Positions 3-314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0207.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.
PhylomeDBiP67046.

Family and domain databases

Gene3Di3.30.572.10. 2 hits.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P67046-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNSFDAAYH SLCEEVLEIG NTRNDRTNTG TISKFGHQLR FDLSKGFPLL
60 70 80 90 100
TTKKVSFKLV ATELLWFIKG DTNIQYLLKY NNNIWNEWAF ENYIKSDEYN
110 120 130 140 150
GPDMTDFGHR ALSDPEFNEQ YKEQMKQFKQ RILEDDTFAK QFGDLGNVYG
160 170 180 190 200
KQWRDWVDKD GNHFDQLKTV IEQIKHNPDS RRHIVSAWNP TEIDTMALPP
210 220 230 240 250
CHTMFQFYVQ DGKLSCQLYQ RSADIFLGVP FNIASYALLT HLIAKECGLE
260 270 280 290 300
VGEFVHTFGD AHIYSNHIDA IQTQLARESF NPPTLKINSD KSIFDINYED
310
LEIVDYESHP AIKAPIAV
Length:318
Mass (Da):36,825
Last modified:October 11, 2004 - v1
Checksum:iE7F41BA915C25D37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57589.1.
RefSeqiNP_371951.1. NC_002758.2.
WP_000934894.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB57589; BAB57589; SAV1427.
KEGGisav:SAV1427.
PATRICi19563578. VBIStaAur52173_1466.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57589.1.
RefSeqiNP_371951.1. NC_002758.2.
WP_000934894.1. NC_002758.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DQ1X-ray2.71A/B1-318[»]
ProteinModelPortaliP67046.
SMRiP67046. Positions 3-314.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV1427.

2D gel databases

World-2DPAGE0002:P67046.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB57589; BAB57589; SAV1427.
KEGGisav:SAV1427.
PATRICi19563578. VBIStaAur52173_1466.

Phylogenomic databases

eggNOGiCOG0207.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.
PhylomeDBiP67046.

Enzyme and pathway databases

UniPathwayiUPA00575.
BioCyciSAUR158878:GJJ5-1443-MONOMER.

Family and domain databases

Gene3Di3.30.572.10. 2 hits.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Mu50 / ATCC 700699.

Entry informationi

Entry nameiTYSY_STAAM
AccessioniPrimary (citable) accession number: P67046
Secondary accession number(s): Q99U61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: June 24, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.