Skip Header

Contribute Send feedback
Read comments (?) or add your own

P67044 (TYSY_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:Rv2764c, MT2834
ORF Names:MTV002.29c
OrganismMycobacterium tuberculosis
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008

Subunit structure

Homodimer By similarity. HAMAP MF_00008

Subcellular location

Cytoplasm By similarity HAMAP MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Sequence caution

The sequence AAK47153.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological processdTMP biosynthetic process

Inferred from direct assay. Source: MTBBASE

dUMP catabolic process

Inferred from direct assay. Source: MTBBASE

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionthymidylate synthase activity

Inferred from direct assay. Source: MTBBASE

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 263263Thymidylate synthase HAMAP MF_00008
PRO_0000140990

Sites

Active site1461 By similarity

Secondary structure

1 .......................................... 263
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P67044 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: DF48279E3E9EA5F3

FASTA26329,853
        10         20         30         40         50         60 
MTPYEDLLRF VLETGTPKSD RTGTGTRSLF GQQMRYDLSA GFPLLTTKKV HFKSVAYELL 

        70         80         90        100        110        120 
WFLRGDSNIG WLHEHGVTIW DEWASDTGEL GPIYGVQWRS WPAPSGEHID QISAALDLLR 

       130        140        150        160        170        180 
TDPDSRRIIV SAWNVGEIER MALPPCHAFF QFYVADGRLS CQLYQRSADL FLGVPFNIAS 

       190        200        210        220        230        240 
YALLTHMMAA QAGLSVGEFI WTGGDCHIYD NHVEQVRLQL SREPRPYPKL LLADRDSIFE 

       250        260 
YTYEDIVVKN YDPHPAIKAP VAV

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX842580 Genomic DNA. Translation: CAA15560.1.
AE000516 Genomic DNA. Translation: AAK47153.1. Different initiation.
PIRC70881.
RefSeqNP_217280.1. NC_000962.2.
NP_337339.1. NC_002755.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3QJ7X-ray2.50A/B/C/D1-263[»]
ProteinModelPortalP67044.
SMRP67044. Positions 1-263.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000000138; EBMYCP00000000138; EBMYCG00000000138.
EBMYCT00000070853; EBMYCP00000068912; EBMYCG00000070848.
GeneID887728.
925453.
GenomeReviewsGene locus MT2834 in contig AE000516_GR.
Gene locus Rv2764c in contig AL123456_GR.
KEGGmtc:MT2834.
mtu:Rv2764c.
PATRIC18127984. VBIMycTub22151_3091.
TIGRMT2834.

Organism-specific databases

TubercuListRv2764c.

Phylogenomic databases

GeneTreeEBGT00050000017116.
HOGENOMHBG588098.
OMARMALAPC.
PhylomeDBP67044.
ProtClustDBPRK01827.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-15786.

Family and domain databases

HAMAPMF_00008. Thymidy_synth_bact.
[Tree]
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
Gene3DG3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KOK00560.
PANTHERPTHR11549:SF2. Thymidylat_synth_C. 1 hit.
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMsTIGR03284. Thym_sym. 2 hits.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_MYCTU
AccessionPrimary (citable) accession number: P67044
Secondary accession number(s): O33306
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents