P67043 (TYSY_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	BR1399, BS1330_I1393

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00008
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP-Rule MF_00008		PRO_0000140943
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P67043 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: FECAD558B5EDCAFA	FASTA	264	30,330
	10 20 30 40 50 60 MRTYLDLLQH VLDHGVDRDD RTGTGTRSVF GYQMRFDLEE GFPVLTTKKL HLRSIIHELL 70 80 90 100 110 120 WFLKGDTNIA YLKENGVTIW DEWADENGDL GPVYGYQWRS WPAPDGRHID QIANLLKMLH 130 140 150 160 170 180 TNPQSRRLIV SAWNPALVDE MALPPCHCLF QFYVANGRLS CQLYQRSADI FLGVPFNIAS 190 200 210 220 230 240 YALLTMMIAQ VTGLKPGEFI HTLGDAHIYS NHFEQARLQL TRTPKKLPVM HINPDVKDLF 250 260 AFRFEDFRLD GYEADPTIKA PIAV

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN30312.1. CP002997 Genomic DNA. Translation: AEM18728.1.
RefSeq	NP_698397.1. NC_004310.3. YP_005616220.1. NC_017251.1.
3D structure databases
ProteinModelPortal	P67043.
SMR	P67043. Positions 1-264.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR1399.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN30312; AAN30312; BR1399. AEM18728; AEM18728; BS1330_I1393.
GeneID	1167081. 12138432.
KEGG	bms:BR1399. bsi:BS1330_I1393.
PATRIC	17791155. VBIBruSui107850_1424.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HOG000257900.
KO	K00560.
OMA	DTNVAYL.
ProtClustDB	PRK01827.
Enzyme and pathway databases
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 1 hit.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BRUSU

Accession

Primary (citable) accession number: P67043
Secondary accession number(s): G0KB82, Q8YI37

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program