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P67042

- TYSY_BRUME

UniProt

P67042 - TYSY_BRUME

Protein

Thymidylate synthase

Gene

thyA

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    • Comment

    Functioni

    Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei146 – 1461UniRule annotation

    GO - Molecular functioni

    1. thymidylate synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. dTMP biosynthetic process Source: UniProtKB-HAMAP
    2. dTTP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
    Short name:
    TSUniRule annotation
    Short name:
    TSaseUniRule annotation
    Gene namesi
    Name:thyAUniRule annotation
    Ordered Locus Names:BMEI0608
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 264264Thymidylate synthasePRO_0000140942Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224914.BMEI0608.

    Structurei

    Secondary structure

    1
    264
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 1413
    Beta strandi16 – 183
    Beta strandi26 – 3712
    Helixi38 – 403
    Helixi52 – 6413
    Helixi70 – 745
    Helixi81 – 833
    Helixi94 – 1007
    Helixi111 – 12111
    Beta strandi129 – 1313
    Helixi135 – 1384
    Beta strandi146 – 15510
    Beta strandi158 – 16912
    Turni170 – 1734
    Helixi174 – 19219
    Beta strandi195 – 20915
    Helixi210 – 2123
    Helixi213 – 2197
    Beta strandi229 – 2324
    Helixi239 – 2413
    Helixi244 – 2463
    Beta strandi247 – 2504

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3IX6X-ray2.20A/B1-264[»]
    ProteinModelPortaliP67042.
    SMRiP67042. Positions 1-264.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP67042.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000257900.
    KOiK00560.
    OMAiDEWARDD.
    OrthoDBiEOG6K6V53.

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 2 hits.
    PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P67042-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRTYLDLLQH VLDHGVDRDD RTGTGTRSVF GYQMRFDLEE GFPVLTTKKL    50
    HLRSIIHELL WFLKGDTNIA YLKENGVTIW DEWADENGDL GPVYGYQWRS 100
    WPAPDGRHID QIANLLKMLH TNPQSRRLIV SAWNPALVDE MALPPCHCLF 150
    QFYVANGRLS CQLYQRSADI FLGVPFNIAS YALLTMMIAQ VTGLKPGEFI 200
    HTLGDAHIYS NHFEQARLQL TRTPKKLPVM HINPDVKDLF AFRFEDFRLD 250
    GYEADPTIKA PIAV 264
    Length:264
    Mass (Da):30,330
    Last modified:October 11, 2004 - v1
    Checksum:iFECAD558B5EDCAFA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL51789.1.
    PIRiAB3328.
    RefSeqiNP_539525.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL51789; AAL51789; BMEI0608.
    GeneIDi1196319.
    KEGGibme:BMEI0608.
    PATRICi17801330. VBIBruMel146950_2990.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL51789.1 .
    PIRi AB3328.
    RefSeqi NP_539525.1. NC_003317.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3IX6 X-ray 2.20 A/B 1-264 [» ]
    ProteinModelPortali P67042.
    SMRi P67042. Positions 1-264.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI0608.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL51789 ; AAL51789 ; BMEI0608 .
    GeneIDi 1196319.
    KEGGi bme:BMEI0608.
    PATRICi 17801330. VBIBruMel146950_2990.

    Phylogenomic databases

    HOGENOMi HOG000257900.
    KOi K00560.
    OMAi DEWARDD.
    OrthoDBi EOG6K6V53.

    Enzyme and pathway databases

    UniPathwayi UPA00575 .

    Miscellaneous databases

    EvolutionaryTracei P67042.

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 2 hits.
    PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiTYSY_BRUME
    AccessioniPrimary (citable) accession number: P67042
    Secondary accession number(s): Q8YI37
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3