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P67042 (TYSY_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:BMEI0608
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length264 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 264264Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000140942

Sites

Active site1461 By similarity

Secondary structure

....................................... 264
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P67042 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: FECAD558B5EDCAFA

FASTA26430,330
        10         20         30         40         50         60 
MRTYLDLLQH VLDHGVDRDD RTGTGTRSVF GYQMRFDLEE GFPVLTTKKL HLRSIIHELL 

        70         80         90        100        110        120 
WFLKGDTNIA YLKENGVTIW DEWADENGDL GPVYGYQWRS WPAPDGRHID QIANLLKMLH 

       130        140        150        160        170        180 
TNPQSRRLIV SAWNPALVDE MALPPCHCLF QFYVANGRLS CQLYQRSADI FLGVPFNIAS 

       190        200        210        220        230        240 
YALLTMMIAQ VTGLKPGEFI HTLGDAHIYS NHFEQARLQL TRTPKKLPVM HINPDVKDLF 

       250        260 
AFRFEDFRLD GYEADPTIKA PIAV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL51789.1.
PIRAB3328.
RefSeqNP_539525.1. NC_003317.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3IX6X-ray2.20A/B1-264[»]
ProteinModelPortalP67042.
SMRP67042. Positions 1-264.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI0608.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL51789; AAL51789; BMEI0608.
GeneID1196319.
KEGGbme:BMEI0608.
PATRIC17801330. VBIBruMel146950_2990.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000257900.
KOK00560.
OMANLGPVYG.
OrthoDBEOG6K6V53.
ProtClustDBPRK01827.

Enzyme and pathway databases

UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 2 hits.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP67042.

Entry information

Entry nameTYSY_BRUME
AccessionPrimary (citable) accession number: P67042
Secondary accession number(s): Q8YI37
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: February 19, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names