Reviewed, UniProtKB/Swiss-Prot P67042 (TYSY_BRUME)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	BMEI0608

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP MF_00008		PRO_0000140942
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P67042-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: FECAD558B5EDCAFA	FASTA	264	30,330
	10 20 30 40 50 60 MRTYLDLLQH VLDHGVDRDD RTGTGTRSVF GYQMRFDLEE GFPVLTTKKL HLRSIIHELL 70 80 90 100 110 120 WFLKGDTNIA YLKENGVTIW DEWADENGDL GPVYGYQWRS WPAPDGRHID QIANLLKMLH 130 140 150 160 170 180 TNPQSRRLIV SAWNPALVDE MALPPCHCLF QFYVANGRLS CQLYQRSADI FLGVPFNIAS 190 200 210 220 230 240 YALLTMMIAQ VTGLKPGEFI HTLGDAHIYS NHFEQARLQL TRTPKKLPVM HINPDVKDLF 250 260 AFRFEDFRLD GYEADPTIKA PIAV

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009503 Genomic DNA. Translation: AAL51789.1.
PIR	AB3328.
RefSeq	NP_539525.1.
3D structure databases
HSSP	HSSP built from PDB template 1TYS based on UniProtKB P00470.
ModBase	Search...
Genome annotation databases
GeneID	1196319.
GenomeReviews	Gene locus BMEI0608 in contig AE008917_GR.
KEGG	bme:BMEI0608.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P67042.
OMA	P67042. LGPVYGH.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0608-MON.
BRENDA	2.1.1.45. 277959.
Family and domain databases
HAMAP	MF_00008. [Tree]
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BRUME

Accession

Primary (citable) accession number: P67042
Secondary accession number(s): Q8YI37

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)