Reviewed, UniProtKB/Swiss-Prot P67018 (SYC2_MYCBO)

Names and origin

Protein names

Recommended name:
    Putative cysteinyl-tRNA synthetase 2
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase 2
      Short name=CysRS 2

Gene names

Name:	cysS2
Ordered Locus Names:	Mb2154c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00041
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	Putative cysteinyl-tRNA synthetase 2 HAMAP MF_00041		PRO_0000159436
Regions
	Motif	46 – 56	11	"HIGH" region HAMAP MF_00041
	Motif	291 – 295	5	"KMSKS" region HAMAP MF_00041
Sites
	Binding site	294	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P67018-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: B03159DB99B871E7	FASTA	414	45,595
	10 20 30 40 50 60 MQSWYCPPVP VLPGRGPQLR LYDSADRQVR PVAPGSKATM YVCGITPYDA THLGHAATYV 70 80 90 100 110 120 TFDLIHRLWL DLGHELHYVQ NITDIDDPLF ERADRDGVDW RDLAQAEVAL FCEDMAALRV 130 140 150 160 170 180 LPPQDYVGAT EAIAEMVELI EKMLACGAAY VIDREMGEYQ DIYFRADATL QFGYESGYDR 190 200 210 220 230 240 DTMLRLCEER GGDPRRPGKS DELDALLWRA ARPGEPSWPS PFGPGRPGWH VECAAIALSR 250 260 270 280 290 300 IGSGLDIQGG GSDLIFPHHE FTAAHAECVS GERRFARHYV HAGMIGWDGH KMSKSRGNLV 310 320 330 340 350 360 LVSALRAQDV EPSAVRLGLL AGHYRADRFW SQQVLDEATA RLHRWRTATA LPAGPAAVDV 370 380 390 400 410 VARVRRYLAD DLDTPKAIAA LDGWVTDAVE YGGHDAGAPK LVATAIDALL GVDL

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248341 Genomic DNA. Translation: CAD97007.1.
RefSeq	NP_855803.1.
3D structure databases
HSSP	HSSP built from PDB template 1LI5 based on UniProtKB P21888.
ModBase	Search...
Genome annotation databases
GeneID	1091211.
GenomeReviews	Gene locus Mb2154c in contig BX248333_GR.
KEGG	mbo:Mb2154c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P67018.
OMA	HYVQNVT.
Enzyme and pathway databases
BRENDA	6.1.1.16. 3091.
Family and domain databases
HAMAP	MF_00041. Divergent sequence. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR017812. Mycothiol_biosynthesis_MshC. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR03447. mycothiol_MshC. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC2_MYCBO

Accession

Primary (citable) accession number: P67018
Secondary accession number(s): O33264

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)