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Protein

Anthranilate synthase component 1

Gene

trpE

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia (By similarity).By similarity

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Enzyme regulationi

Feedback inhibited by tryptophan.By similarity

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component 1 (trpE)
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. Phosphoribosyl isomerase A (priA)
  4. Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561TryptophanBy similarity
Metal bindingi351 – 3511MagnesiumBy similarity
Binding sitei439 – 4391ChorismateBy similarity
Binding sitei459 – 4591ChorismateBy similarity
Binding sitei475 – 4751Chorismate; via amide nitrogenBy similarity
Metal bindingi488 – 4881MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 1 (EC:4.1.3.27)
Short name:
AS
Short name:
ASI
Gene namesi
Name:trpE
Ordered Locus Names:Mb1635
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 516516Anthranilate synthase component 1PRO_0000154100Add
BLAST

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).By similarity

Structurei

3D structure databases

ProteinModelPortaliP67002.
SMRiP67002. Positions 3-511.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni283 – 2853Tryptophan bindingBy similarity
Regioni324 – 3252Chorismate bindingBy similarity
Regioni473 – 4753Chorismate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000025142.
KOiK01657.
OMAiCEELAVI.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005256. Anth_synth_I_PabB.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00564. trpE_most. 1 hit.

Sequencei

Sequence statusi: Complete.

P67002-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHADLAATTS REDFRLLAAE HRVVPVTRKV LADSETPLSA YRKLAANRPG
60 70 80 90 100
TFLLESAENG RSWSRWSFIG AGAPTALTVR EGQAVWLGAV PKDAPTGGDP
110 120 130 140 150
LRALQVTLEL LATADRQSEP GLPPLSGGMV GFFAYDMVRR LERLPERAVD
160 170 180 190 200
DLCLPDMLLL LATDVAAVDH HEGTITLIAN AVNWNGTDER VDWAYDDAVA
210 220 230 240 250
RLDVMTAALG QPLPSTVATF SRPEPRHRAQ RTVEEYGAIV EYLVDQIAAG
260 270 280 290 300
EAFQVVPSQR FEMDTDVDPI DVYRILRVTN PSPYMYLLQV PNSDGAVDFS
310 320 330 340 350
IVGSSPEALV TVHEGWATTH PIAGTRWRGR TDDEDVLLEK ELLADDKERA
360 370 380 390 400
EHLMLVDLGR NDLGRVCTPG TVRVEDYSHI ERYSHVMHLV STVTGKLGEG
410 420 430 440 450
RTALDAVTAC FPAGTLSGAP KVRAMELIEE VEKTRRGLYG GVVGYLDFAG
460 470 480 490 500
NADFAIAIRT ALMRNGTAYV QAGGGVVADS NGSYEYNEAR NKARAVLNAI
510
AAAETLAAPG ANRSGC
Length:516
Mass (Da):55,849
Last modified:October 11, 2004 - v1
Checksum:iEF19CDDE80E802C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO42884.1.
RefSeqiNP_855288.1. NC_002945.3.
WP_003407977.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO42884; CDO42884; Mb1635.
GeneIDi1092566.
KEGGimbo:Mb1635.
PATRICi18005273. VBIMycBov88188_1784.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO42884.1.
RefSeqiNP_855288.1. NC_002945.3.
WP_003407977.1. NC_002945.3.

3D structure databases

ProteinModelPortaliP67002.
SMRiP67002. Positions 3-511.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO42884; CDO42884; Mb1635.
GeneIDi1092566.
KEGGimbo:Mb1635.
PATRICi18005273. VBIMycBov88188_1784.

Phylogenomic databases

HOGENOMiHOG000025142.
KOiK01657.
OMAiCEELAVI.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiIPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005256. Anth_synth_I_PabB.
IPR015890. Chorismate_C.
[Graphical view]
PfamiPF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
[Graphical view]
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00564. trpE_most. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRPE_MYCBO
AccessioniPrimary (citable) accession number: P67002
Secondary accession number(s): O06127, X2BIC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.