Reviewed, UniProtKB/Swiss-Prot P67001 (TRPE_MYCTU)

Names and origin

Protein names

Recommended name:
    Anthranilate synthase component 1
    EC=4.1.3.27
Alternative name(s):
    Anthranilate synthase component I

Gene names

Name:	trpE
Ordered Locus Names:	Rv1609, MT1644
ORF Names:	MTCY01B2.01, MTV046.07

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II By similarity.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Belongs to the anthranilate synthase component I family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 516	516	Anthranilate synthase component 1		PRO_0000154099

Sequences

	Sequence		Length	Mass (Da)
	P67001-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: EF19CDDE80E802C7	FASTA	516	55,849
	10 20 30 40 50 60 MHADLAATTS REDFRLLAAE HRVVPVTRKV LADSETPLSA YRKLAANRPG TFLLESAENG 70 80 90 100 110 120 RSWSRWSFIG AGAPTALTVR EGQAVWLGAV PKDAPTGGDP LRALQVTLEL LATADRQSEP 130 140 150 160 170 180 GLPPLSGGMV GFFAYDMVRR LERLPERAVD DLCLPDMLLL LATDVAAVDH HEGTITLIAN 190 200 210 220 230 240 AVNWNGTDER VDWAYDDAVA RLDVMTAALG QPLPSTVATF SRPEPRHRAQ RTVEEYGAIV 250 260 270 280 290 300 EYLVDQIAAG EAFQVVPSQR FEMDTDVDPI DVYRILRVTN PSPYMYLLQV PNSDGAVDFS 310 320 330 340 350 360 IVGSSPEALV TVHEGWATTH PIAGTRWRGR TDDEDVLLEK ELLADDKERA EHLMLVDLGR 370 380 390 400 410 420 NDLGRVCTPG TVRVEDYSHI ERYSHVMHLV STVTGKLGEG RTALDAVTAC FPAGTLSGAP 430 440 450 460 470 480 KVRAMELIEE VEKTRRGLYG GVVGYLDFAG NADFAIAIRT ALMRNGTAYV QAGGGVVADS 490 500 510 NGSYEYNEAR NKARAVLNAI AAAETLAAPG ANRSGC

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842577 Genomic DNA. Translation: CAB08903.1. AE000516 Genomic DNA. Translation: AAK45913.1.
PIR	G70556.
RefSeq	NP_216125.1. NP_336099.1.
3D structure databases
HSSP	HSSP built from PDB template 1K0E based on UniProtKB P05041.
ModBase	Search...
Genome annotation databases
GeneID	885040. 924261.
GenomeReviews	Gene locus MT1644 in contig AE000516_GR. Gene locus Rv1609 in contig AL123456_GR.
KEGG	mtc:MT1644. mtu:Rv1609.
TIGR	MT1644.
Organism-specific databases
TubercuList	Rv1609.
Phylogenomic databases
HOGENOM	P67001.
OMA	RFSILAY.
Enzyme and pathway databases
BRENDA	4.1.3.27. 809.
Family and domain databases
InterPro	IPR005801. ADC_synthase. IPR006805. Anth_synth_I_N. IPR005256. Anth_synthI. IPR019999. Anthranilate_synth_I_C. IPR015890. Chorismate-bd_C. [Graphical view]
Gene3D	G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
PANTHER	PTHR11236. TRPE_1_chor_bd. 1 hit.
Pfam	PF04715. Anth_synt_I_N. 1 hit. PF00425. Chorismate_bind. 1 hit. [Graphical view]
PRINTS	PR00095. ANTSNTHASEI.
ProDom	PD000779. Anth_synth_chor. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00564. trpE_most. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRPE_MYCTU

Accession

Primary (citable) accession number: P67001
Secondary accession number(s): O06127

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)