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Protein

Tryptophan synthase beta chain

Gene

trpB

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.By similarity

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

Cofactori

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component 1 (trpE)
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. Phosphoribosyl isomerase A (priA)
  4. Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase beta chain (EC:4.2.1.20)
Gene namesi
Name:trpB
Ordered Locus Names:Mb1638
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000989701 – 422Tryptophan synthase beta chainAdd BLAST422

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei113N6-(pyridoxal phosphate)lysineBy similarity1

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.By similarity

Structurei

3D structure databases

ProteinModelPortaliP66985.
SMRiP66985.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpB family.Curated

Phylogenomic databases

HOGENOMiHOG000161710.
KOiK01696.

Family and domain databases

CDDicd06446. Trp-synth_B. 1 hit.
HAMAPiMF_00133. Trp_synth_beta. 1 hit.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P66985-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMTDLSTPDL PRMSAAIAEP TSHDPDSGGH FGGPSGWGGR YVPEALMAVI
60 70 80 90 100
EEVTAAYQKE RVSQDFLDDL DRLQANYAGR PSPLYEATRL SQHAGSARIF
110 120 130 140 150
LKREDLNHTG SHKINNVLGQ ALLARRMGKT RVIAETGAGQ HGVATATACA
160 170 180 190 200
LLGLDCVIYM GGIDTARQAL NVARMRLLGA EVVAVQTGSK TLKDAINEAF
210 220 230 240 250
RDWVANADNT YYCFGTAAGP HPFPTMVRDF QRIIGMEARV QIQGQAGRLP
260 270 280 290 300
DAVVACVGGG SNAIGIFHAF LDDPGVRLVG FEAAGDGVET GRHAATFTAG
310 320 330 340 350
SPGAFHGSFS YLLQDEDGQT IESHSISAGL DYPGVGPEHA WLKEAGRVDY
360 370 380 390 400
RPITDSEAMD AFGLLCRMEG IIPAIESAHA VAGALKLGVE LGRGAVIVVN
410 420
LSGRGDKDVE TAAKWFGLLG ND
Length:422
Mass (Da):44,645
Last modified:October 11, 2004 - v1
Checksum:i7976DA2DC85D5E78
GO

Sequence cautioni

The sequence CDO42887 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO42887.1. Different initiation.
RefSeqiNP_855291.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO42887; CDO42887; Mb1638.
GeneIDi1092570.
KEGGimbo:Mb1638.
PATRICi18005279. VBIMycBov88188_1787.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO42887.1. Different initiation.
RefSeqiNP_855291.1. NC_002945.3.

3D structure databases

ProteinModelPortaliP66985.
SMRiP66985.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO42887; CDO42887; Mb1638.
GeneIDi1092570.
KEGGimbo:Mb1638.
PATRICi18005279. VBIMycBov88188_1787.

Phylogenomic databases

HOGENOMiHOG000161710.
KOiK01696.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.

Family and domain databases

CDDicd06446. Trp-synth_B. 1 hit.
HAMAPiMF_00133. Trp_synth_beta. 1 hit.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPB_MYCBO
AccessioniPrimary (citable) accession number: P66985
Secondary accession number(s): O08376, X2BI05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 30, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.