P66968 (TRMD_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	Rv2906c, MT2974
ORF Names:	MTCY274.37c

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Miscellaneous	Was identified as a high-confidence drug target. HAMAP MF_00605
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: MTBBASE
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060410
Regions
	Region	133 – 138	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	109	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P66968 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 5981C3D149ED0AC5	FASTA	230	25,166
	10 20 30 40 50 60 MRIDIVTIFP ACLDPLRQSL PGKAIESGLV DLNVHDLRRW THDVHHSVDD APYGGGPGMV 70 80 90 100 110 120 MKAPVWGEAL DEICSSETLL IVPTPAGVLF TQATAQRWTT ESHLVFACGR YEGIDQRVVQ 130 140 150 160 170 180 DAARRMRVEE VSIGDYVLPG GESAAVVMVE AVLRLLAGVL GNPASHQDDS HSTGLDGLLE 190 200 210 220 230 GPSYTRPASW RGLDVPEVLL SGDHARIAAW RREVSLQRTR ERRPDLSHPD

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842581 Genomic DNA. Translation: CAA98343.1. AE000516 Genomic DNA. Translation: AAK47300.1.
PIR	E70927.
RefSeq	NP_217422.1. NC_000962.2. NP_337486.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P66968.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000003184; EBMYCP00000003184; EBMYCG00000003182. EBMYCT00000069551; EBMYCP00000067610; EBMYCG00000069546.
GeneID	888495. 925304.
GenomeReviews	Gene locus MT2974 in contig AE000516_GR. Gene locus Rv2906c in contig AL123456_GR.
KEGG	mtc:MT2974. mtu:Rv2906c.
PATRIC	18128308. VBIMycTub22151_3253.
TIGR	MT2974.
Organism-specific databases
TubercuList	Rv2906c.
Phylogenomic databases
GeneTree	EBGT00050000016420.
HOGENOM	HBG285805.
OMA	VCGRFEG.
PhylomeDB	P66968.
ProtClustDB	PRK00026.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_MYCTU

Accession

Primary (citable) accession number: P66968
Secondary accession number(s): Q10797

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program