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Protein

Beta-barrel assembly-enhancing protease

Gene

bepA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as both a chaperone and a metalloprotease. Maintains the integrity of the outer membrane by promoting either the assembly or the elimination of outer membrane proteins, depending on their folding state. Promotes disulfide rearrangement of LptD during its biogenesis, and proteolytic degradation of LptD and BamA when their proper assembly is compromised. May facilitate membrane attachment of LoiP under unfavorable conditions.UniRule annotation2 Publications

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Enzyme regulationi

Protease activity is inhibited by the metal chelating reagents 1,10-phenanthroline and EDTA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi136Zinc; catalyticUniRule annotation1
Active sitei137UniRule annotation1
Metal bindingi140Zinc; catalyticUniRule annotation1
Metal bindingi201Zinc; catalyticUniRule annotation1
Active sitei205Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • chaperone-mediated protein folding Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7311-MONOMER.

Protein family/group databases

MEROPSiM48.A12.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-barrel assembly-enhancing proteaseUniRule annotation (EC:3.4.-.-UniRule annotation)
Gene namesi
Name:bepAUniRule annotation
Synonyms:yfgC
Ordered Locus Names:b2494, JW2479
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14199. bepA.

Subcellular locationi

P66948:

GO - Cellular componenti

  • membrane Source: EcoCyc
  • outer membrane-bounded periplasmic space Source: EcoCyc

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Disruption sensitizes cells to multiple drugs.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi136H → R: Lack of protease activity. Increases sensitivity to erythromycin. 1 Publication1
Mutagenesisi137E → Q: Lack of protease activity. Increases sensitivity to erythromycin. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27UniRule annotationAdd BLAST27
ChainiPRO_000003569628 – 487Beta-barrel assembly-enhancing proteaseAdd BLAST460

Proteomic databases

PaxDbiP66948.
PRIDEiP66948.

Interactioni

Subunit structurei

Interacts with BamA and LoiP.2 Publications

Protein-protein interaction databases

BioGridi4261434. 32 interactors.
DIPiDIP-28089N.
IntActiP66948. 3 interactors.
STRINGi316385.ECDH10B_2660.

Structurei

3D structure databases

ProteinModelPortaliP66948.
SMRiP66948.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati309 – 342TPR 1Add BLAST34
Repeati344 – 376TPR 2Add BLAST33
Repeati377 – 409TPR 3Add BLAST33
Repeati427 – 460TPR 4Add BLAST34

Sequence similaritiesi

Belongs to the peptidase M48 family. BepA subfamily.UniRule annotation

Keywords - Domaini

Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiENOG4105ERG. Bacteria.
COG4783. LUCA.
HOGENOMiHOG000264031.
InParanoidiP66948.
PhylomeDBiP66948.

Family and domain databases

Gene3Di1.25.20.10. 1 hit.
HAMAPiMF_00997. Protease_BepA. 1 hit.
InterProiView protein in InterPro
IPR008939. Lytic_TGlycosylase_superhlx_U.
IPR001915. Peptidase_M48.
IPR030873. Protease_BepA.
IPR011990. TPR-like_helical_dom.
PfamiView protein in Pfam
PF01435. Peptidase_M48. 1 hit.
SUPFAMiSSF48452. SSF48452. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P66948-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRQLKKNLV ATLIAAMTIG QVAPAFADSA DTLPDMGTSA GSTLSIGQEM
60 70 80 90 100
QMGDYYVRQL RGSAPLINDP LLTQYINSLG MRLVSHANSV KTPFHFFLIN
110 120 130 140 150
NDEINAFAFF GGNVVLHSAL FRYSDNESQL ASVMAHEISH VTQRHLARAM
160 170 180 190 200
EDQQRSAPLT WVGALGSILL AMASPQAGMA ALTGTLAGTR QGMISFTQQN
210 220 230 240 250
EQEADRIGIQ VLQRSGFDPQ AMPTFLEKLL DQARYSSRPP EILLTHPLPE
260 270 280 290 300
SRLADARNRA NQMRPMVVQS SEDFYLAKAR TLGMYNSGRN QLTSDLLDEW
310 320 330 340 350
AKGNVRQQRA AQYGRALQAM EANKYDEARK TLQPLLAAEP GNAWYLDLAT
360 370 380 390 400
DIDLGQNKAN EAINRLKNAR DLRTNPVLQL NLANAYLQGG QPQEAANILN
410 420 430 440 450
RYTFNNKDDS NGWDLLAQAE AALNNRDQEL AARAEGYALA GRLDQAISLL
460 470 480
SSASSQVKLG SLQQARYDAR IDQLRQLQER FKPYTKM
Length:487
Mass (Da):53,908
Last modified:October 11, 2004 - v1
Checksum:i92A6E6BAA370A625
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75547.1.
AP009048 Genomic DNA. Translation: BAE76724.1.
PIRiE65025.
RefSeqiNP_416989.1. NC_000913.3.
WP_000489667.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75547; AAC75547; b2494.
BAE76724; BAE76724; BAE76724.
GeneIDi947029.
KEGGiecj:JW2479.
eco:b2494.
PATRICifig|511145.12.peg.2590.

Similar proteinsi

Entry informationi

Entry nameiBEPA_ECOLI
AccessioniPrimary (citable) accession number: P66948
Secondary accession number(s): P76568, Q2MAI2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: October 25, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families