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Protein

Beta-barrel assembly-enhancing protease

Gene

bepA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as both a chaperone and a metalloprotease. Maintains the integrity of the outer membrane by promoting either the assembly or the elimination of outer membrane proteins, depending on their folding state. Promotes disulfide rearrangement of LptD during its biogenesis, and proteolytic degradation of LptD and BamA when their proper assembly is compromised. May facilitate membrane attachment of LoiP under unfavorable conditions.UniRule annotation2 Publications

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Enzyme regulationi

Protease activity is inhibited by the metal chelating reagents 1,10-phenanthroline and EDTA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi136Zinc; catalyticUniRule annotation1
Active sitei137UniRule annotation1
Metal bindingi140Zinc; catalyticUniRule annotation1
Metal bindingi201Zinc; catalyticUniRule annotation1
Active sitei205Proton donorUniRule annotation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: InterPro
  • metallopeptidase activity Source: EcoCyc

GO - Biological processi

  • chaperone-mediated protein folding Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7311-MONOMER
MetaCyc:G7311-MONOMER

Protein family/group databases

MEROPSiM48.A12

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-barrel assembly-enhancing proteaseUniRule annotation (EC:3.4.-.-UniRule annotation)
Gene namesi
Name:bepAUniRule annotation
Synonyms:yfgC
Ordered Locus Names:b2494, JW2479
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14199 bepA

Subcellular locationi

GO - Cellular componenti

  • membrane Source: EcoCyc
  • outer membrane-bounded periplasmic space Source: EcoCyc

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Disruption sensitizes cells to multiple drugs.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi136H → R: Lack of protease activity. Increases sensitivity to erythromycin. 1 Publication1
Mutagenesisi137E → Q: Lack of protease activity. Increases sensitivity to erythromycin. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27UniRule annotationAdd BLAST27
ChainiPRO_000003569628 – 487Beta-barrel assembly-enhancing proteaseAdd BLAST460

Proteomic databases

EPDiP66948
PaxDbiP66948
PRIDEiP66948

Interactioni

Subunit structurei

Interacts with BamA and LoiP.2 Publications

Protein-protein interaction databases

BioGridi4261434, 45 interactors
DIPiDIP-28089N
IntActiP66948, 3 interactors
STRINGi316385.ECDH10B_2660

Structurei

Secondary structure

1487
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi310 – 321Combined sources12
Helixi325 – 338Combined sources14
Helixi343 – 355Combined sources13
Helixi359 – 367Combined sources9
Helixi370 – 374Combined sources5
Helixi376 – 388Combined sources13
Helixi392 – 405Combined sources14
Helixi410 – 422Combined sources13
Helixi426 – 439Combined sources14
Helixi443 – 456Combined sources14
Helixi462 – 479Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5XI8X-ray1.70A310-482[»]
ProteinModelPortaliP66948
SMRiP66948
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati309 – 342TPR 1Add BLAST34
Repeati344 – 376TPR 2Add BLAST33
Repeati377 – 409TPR 3Add BLAST33
Repeati427 – 460TPR 4Add BLAST34

Sequence similaritiesi

Belongs to the peptidase M48 family. BepA subfamily.UniRule annotation

Keywords - Domaini

Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiENOG4105ERG Bacteria
COG4783 LUCA
HOGENOMiHOG000264031
InParanoidiP66948
OMAiHLSQRHF
PhylomeDBiP66948

Family and domain databases

Gene3Di1.25.40.10, 1 hit
HAMAPiMF_00997 Protease_BepA, 1 hit
InterProiView protein in InterPro
IPR001915 Peptidase_M48
IPR030873 Protease_BepA
IPR011990 TPR-like_helical_dom_sf
PfamiView protein in Pfam
PF01435 Peptidase_M48, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P66948-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRQLKKNLV ATLIAAMTIG QVAPAFADSA DTLPDMGTSA GSTLSIGQEM
60 70 80 90 100
QMGDYYVRQL RGSAPLINDP LLTQYINSLG MRLVSHANSV KTPFHFFLIN
110 120 130 140 150
NDEINAFAFF GGNVVLHSAL FRYSDNESQL ASVMAHEISH VTQRHLARAM
160 170 180 190 200
EDQQRSAPLT WVGALGSILL AMASPQAGMA ALTGTLAGTR QGMISFTQQN
210 220 230 240 250
EQEADRIGIQ VLQRSGFDPQ AMPTFLEKLL DQARYSSRPP EILLTHPLPE
260 270 280 290 300
SRLADARNRA NQMRPMVVQS SEDFYLAKAR TLGMYNSGRN QLTSDLLDEW
310 320 330 340 350
AKGNVRQQRA AQYGRALQAM EANKYDEARK TLQPLLAAEP GNAWYLDLAT
360 370 380 390 400
DIDLGQNKAN EAINRLKNAR DLRTNPVLQL NLANAYLQGG QPQEAANILN
410 420 430 440 450
RYTFNNKDDS NGWDLLAQAE AALNNRDQEL AARAEGYALA GRLDQAISLL
460 470 480
SSASSQVKLG SLQQARYDAR IDQLRQLQER FKPYTKM
Length:487
Mass (Da):53,908
Last modified:October 11, 2004 - v1
Checksum:i92A6E6BAA370A625
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75547.1
AP009048 Genomic DNA Translation: BAE76724.1
PIRiE65025
RefSeqiNP_416989.1, NC_000913.3
WP_000489667.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75547; AAC75547; b2494
BAE76724; BAE76724; BAE76724
GeneIDi947029
KEGGiecj:JW2479
eco:b2494
PATRICifig|511145.12.peg.2590

Similar proteinsi

Entry informationi

Entry nameiBEPA_ECOLI
AccessioniPrimary (citable) accession number: P66948
Secondary accession number(s): P76568, Q2MAI2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health