Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-barrel assembly-enhancing protease

Gene

bepA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as both a chaperone and a metalloprotease. Maintains the integrity of the outer membrane by promoting either the assembly or the elimination of outer membrane proteins, depending on their folding state. Promotes disulfide rearrangement of LptD during its biogenesis, and proteolytic degradation of LptD and BamA when their proper assembly is compromised. May facilitate membrane attachment of LoiP under unfavorable conditions.UniRule annotation2 Publications

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Enzyme regulationi

Protease activity is inhibited by the metal chelating reagents 1,10-phenanthroline and EDTA.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi136 – 1361Zinc; catalyticUniRule annotation
Active sitei137 – 1371UniRule annotation
Metal bindingi140 – 1401Zinc; catalyticUniRule annotation
Metal bindingi201 – 2011Zinc; catalyticUniRule annotation
Active sitei205 – 2051Proton donorUniRule annotation

GO - Molecular functioni

  • metalloendopeptidase activity Source: InterPro
  • metallopeptidase activity Source: EcoCyc
  • zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  • chaperone-mediated protein folding Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7311-MONOMER.
ECOL316407:JW2479-MONOMER.

Protein family/group databases

MEROPSiM48.A12.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-barrel assembly-enhancing proteaseUniRule annotation (EC:3.4.-.-UniRule annotation)
Gene namesi
Name:bepAUniRule annotation
Synonyms:yfgC
Ordered Locus Names:b2494, JW2479
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14199. bepA.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: EcoCyc
  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Disruption sensitizes cells to multiple drugs.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi136 – 1361H → R: Lack of protease activity. Increases sensitivity to erythromycin. 1 Publication
Mutagenesisi137 – 1371E → Q: Lack of protease activity. Increases sensitivity to erythromycin. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727UniRule annotationAdd
BLAST
Chaini28 – 487460Beta-barrel assembly-enhancing proteasePRO_0000035696Add
BLAST

Proteomic databases

EPDiP66948.
PaxDbiP66948.
PRIDEiP66948.

Interactioni

Subunit structurei

Interacts with BamA and LoiP.2 Publications

Protein-protein interaction databases

BioGridi4261434. 8 interactions.
DIPiDIP-28089N.
IntActiP66948. 3 interactions.
STRINGi511145.b2494.

Structurei

3D structure databases

ProteinModelPortaliP66948.
SMRiP66948. Positions 44-280, 321-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati309 – 34234TPR 1Add
BLAST
Repeati344 – 37633TPR 2Add
BLAST
Repeati377 – 40933TPR 3Add
BLAST
Repeati427 – 46034TPR 4Add
BLAST

Sequence similaritiesi

Belongs to the peptidase M48 family. BepA subfamily.UniRule annotation
Contains 4 TPR repeats.UniRule annotation

Keywords - Domaini

Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiENOG4105ERG. Bacteria.
COG4783. LUCA.
HOGENOMiHOG000264031.
InParanoidiP66948.
OMAiDPQLKDY.
OrthoDBiEOG6PP9PB.
PhylomeDBiP66948.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
HAMAPiMF_00997. Protease_BepA.
InterProiIPR001915. Peptidase_M48.
IPR030873. Protease_BepA.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF01435. Peptidase_M48. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P66948-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRQLKKNLV ATLIAAMTIG QVAPAFADSA DTLPDMGTSA GSTLSIGQEM
60 70 80 90 100
QMGDYYVRQL RGSAPLINDP LLTQYINSLG MRLVSHANSV KTPFHFFLIN
110 120 130 140 150
NDEINAFAFF GGNVVLHSAL FRYSDNESQL ASVMAHEISH VTQRHLARAM
160 170 180 190 200
EDQQRSAPLT WVGALGSILL AMASPQAGMA ALTGTLAGTR QGMISFTQQN
210 220 230 240 250
EQEADRIGIQ VLQRSGFDPQ AMPTFLEKLL DQARYSSRPP EILLTHPLPE
260 270 280 290 300
SRLADARNRA NQMRPMVVQS SEDFYLAKAR TLGMYNSGRN QLTSDLLDEW
310 320 330 340 350
AKGNVRQQRA AQYGRALQAM EANKYDEARK TLQPLLAAEP GNAWYLDLAT
360 370 380 390 400
DIDLGQNKAN EAINRLKNAR DLRTNPVLQL NLANAYLQGG QPQEAANILN
410 420 430 440 450
RYTFNNKDDS NGWDLLAQAE AALNNRDQEL AARAEGYALA GRLDQAISLL
460 470 480
SSASSQVKLG SLQQARYDAR IDQLRQLQER FKPYTKM
Length:487
Mass (Da):53,908
Last modified:October 11, 2004 - v1
Checksum:i92A6E6BAA370A625
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75547.1.
AP009048 Genomic DNA. Translation: BAE76724.1.
PIRiE65025.
RefSeqiNP_416989.1. NC_000913.3.
WP_000489667.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75547; AAC75547; b2494.
BAE76724; BAE76724; BAE76724.
GeneIDi947029.
KEGGiecj:JW2479.
eco:b2494.
PATRICi32120375. VBIEscCol129921_2590.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75547.1.
AP009048 Genomic DNA. Translation: BAE76724.1.
PIRiE65025.
RefSeqiNP_416989.1. NC_000913.3.
WP_000489667.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP66948.
SMRiP66948. Positions 44-280, 321-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261434. 8 interactions.
DIPiDIP-28089N.
IntActiP66948. 3 interactions.
STRINGi511145.b2494.

Protein family/group databases

MEROPSiM48.A12.

Proteomic databases

EPDiP66948.
PaxDbiP66948.
PRIDEiP66948.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75547; AAC75547; b2494.
BAE76724; BAE76724; BAE76724.
GeneIDi947029.
KEGGiecj:JW2479.
eco:b2494.
PATRICi32120375. VBIEscCol129921_2590.

Organism-specific databases

EchoBASEiEB3951.
EcoGeneiEG14199. bepA.

Phylogenomic databases

eggNOGiENOG4105ERG. Bacteria.
COG4783. LUCA.
HOGENOMiHOG000264031.
InParanoidiP66948.
OMAiDPQLKDY.
OrthoDBiEOG6PP9PB.
PhylomeDBiP66948.

Enzyme and pathway databases

BioCyciEcoCyc:G7311-MONOMER.
ECOL316407:JW2479-MONOMER.

Miscellaneous databases

PROiP66948.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
HAMAPiMF_00997. Protease_BepA.
InterProiIPR001915. Peptidase_M48.
IPR030873. Protease_BepA.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF01435. Peptidase_M48. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "E. coli LoiP (YggG), a metalloprotease hydrolyzing Phe-Phe bonds."
    Lutticke C., Hauske P., Lewandrowski U., Sickmann A., Kaiser M., Ehrmann M.
    Mol. Biosyst. 8:1775-1782(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH LOIP, SUBCELLULAR LOCATION.
  4. "Protease homolog BepA (YfgC) promotes assembly and degradation of beta-barrel membrane proteins in Escherichia coli."
    Narita S., Masui C., Suzuki T., Dohmae N., Akiyama Y.
    Proc. Natl. Acad. Sci. U.S.A. 110:E3612-E3621(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A CHAPERONE AND A PROTEASE, ENZYME REGULATION, INTERACTION WITH BAMA, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, GENE NAME, MUTAGENESIS OF HIS-136 AND GLU-137.
    Strain: K12.

Entry informationi

Entry nameiBEPA_ECOLI
AccessioniPrimary (citable) accession number: P66948
Secondary accession number(s): P76568, Q2MAI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 16, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.