Reviewed, UniProtKB/Swiss-Prot P66926 (THIL_MYCTU)

Names and origin

Protein names

Recommended name:
    Probable acetyl-CoA acetyltransferase
    EC=2.3.1.9
Alternative name(s):
    Acetoacetyl-CoA thiolase

Gene names

Name:	fadA4
Ordered Locus Names:	Rv1323, MT1365
ORF Names:	MTCY130.08

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Probable acetyl-CoA acetyltransferase		PRO_0000206458
Sites
	Active site	84	1	Acyl-thioester intermediate By similarity
	Active site	345	1	Proton acceptor By similarity
	Active site	375	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P66926-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 32F892A90364AB05	FASTA	389	40,081
	10 20 30 40 50 60 MIVAGARTPI GKLMGSLKDF SASELGAIAI KGALEKANVP ASLVEYVIMG QVLTAGAGQM 70 80 90 100 110 120 PARQAAVAAG IGWDVPALTI NKMCLSGIDA IALADQLIRA REFDVVVAGG QESMTKAPHL 130 140 150 160 170 180 LMNSRSGYKY GDVTVLDHMA YDGLHDVFTD QPMGALTEQR NDVDMFTRSE QDEYAAASHQ 190 200 210 220 230 240 KAAAAWKDGV FADEVIPVNI PQRTGDPLQF TEDEGIRANT TAAALAGLKP AFRGDGTITA 250 260 270 280 290 300 GSASQISDGA AAVVVMNQEK AQELGLTWLA EIGAHGVVAG PDSTLQSQPA NAINKALDRE 310 320 330 340 350 360 GISVDQLDVV EINEAFAAVA LASIRELGLN PQIVNVNGGA IAVGHPLGMS GTRITLHAAL 370 380 QLARRGSGVG VAALCGAGGQ GDALILRAG

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842576 Genomic DNA. Translation: CAA98087.1. AE000516 Genomic DNA. Translation: AAK45628.1.
PIR	G70769.
RefSeq	NP_215839.1. NP_335814.1.
3D structure databases
HSSP	HSSP built from PDB template 1PXT based on UniProtKB P27796.
ModBase	Search...
Genome annotation databases
GeneID	886904. 924693.
GenomeReviews	Gene locus MT1365 in contig AE000516_GR. Gene locus Rv1323 in contig AL123456_GR.
KEGG	mtc:MT1365. mtu:Rv1323.
TIGR	MT1365.
Organism-specific databases
TubercuList	Rv1323.
Phylogenomic databases
HOGENOM	P66926.
OMA	DANTHDG.
Enzyme and pathway databases
BRENDA	2.3.1.9. 809.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THIL_MYCTU

Accession

Primary (citable) accession number: P66926
Secondary accession number(s): Q10629

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)