P66922 (THIM_STAAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyethylthiazole kinase
EC=2.7.1.50

Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase

Gene names

Name:	thiM
Ordered Locus Names:	SAV2092

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Cofactor	Magnesium By similarity. HAMAP MF_00228
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_0000156953
Sites
	Binding site	39	1	Substrate; via amide nitrogen By similarity
	Binding site	115	1	ATP By similarity
	Binding site	160	1	ATP By similarity
	Binding site	187	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P66922 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: B7D8DD02F2D8E98E	FASTA	263	28,067
	10 20 30 40 50 60 MNYLNKIRIE NPLTICYTND VVKNFTANGL LSIGASPAMS EAPEEAEEFY KVAQALLINI 70 80 90 100 110 120 GTLTAQNEQD IIAIAQTANE AGLPIVFDPV AVGASTYRKQ FCKLLLKSAK VSVIKGNASE 130 140 150 160 170 180 ILALIDDTAT MKGTDSDANL DAVAIAKKAY AIYKTAIVIT GKEDVIVQDN KAIVLANGSP 190 200 210 220 230 240 LLARVTGAGC LLGGVIAGFL FRETEPDIEA LIEAVSVFNI AAEVAAENEN CGGPGTFSPL 250 260 LLDTLYHLNE TTYQQRIRIQ EVE

References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Mu50 / ATCC 700699.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000017 Genomic DNA. Translation: BAB58254.1.
RefSeq	NP_372616.1. NC_002758.2.
3D structure databases
ProteinModelPortal	P66922.
ModBase	Search...
Protein-protein interaction databases
STRING	P66922.
2D gel databases
World-2DPAGE	0002:P66922.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000007792; EBSTAP00000007610; EBSTAG00000007791.
GeneID	1122109.
GenomeReviews	Gene locus SAV2092 in contig BA000017_GR.
KEGG	sav:SAV2092.
PATRIC	19565062. VBIStaAur52173_2166.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2145.
GeneTree	EBGT00050000025107.
HOGENOM	HBG351126.
OMA	AIRGNAG.
ProtClustDB	PRK09355.
Enzyme and pathway databases
BioCyc	SAUR158878:SAV2092-MONOMER.
Family and domain databases
HAMAP	MF_00228. Thz_kinase. [Tree]
InterPro	IPR000417. Hyethyz_kinase. [Graphical view]
KO	K00878.
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
ProtoNet	Search...

Entry information

Entry name

THIM_STAAM

Accession

Primary (citable) accession number: P66922
Secondary accession number(s): Q99SG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program