P66915 (THID_STAAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
EC=2.7.1.49
EC=2.7.4.7

Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name=HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name=HMP-P kinase
Short name=HMP-phosphate kinase
Short name=HMPP kinase

Gene names

Name:	thiD
Ordered Locus Names:	SAV2093

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P By similarity.
Catalytic activity	ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine. ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 2/3. Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 3/3.
Sequence similarities	Belongs to the thiD family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydroxymethylpyrimidine kinase activity Inferred from electronic annotation. Source: EC phosphomethylpyrimidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase		PRO_0000192028
Sites
	Binding site	45	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P66915 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 4E42A1E6A6622004	FASTA	276	30,242
	10 20 30 40 50 60 MIKPKIALTI AGTDPTGGAG VMADLKSFHS CGVYGMGVVT SIVAQNTLGV QHIHNLNHQW 70 80 90 100 110 120 VDEQLDSVFN DTLPHAIKTG MIATADTMET IRHYLMQHES IPYVIDPVML AKSGDSLMDN 130 140 150 160 170 180 DTKQNLQHTL LPLADVVTPN LPEAEEITGL TIDSEEKIMQ AGRIFINEIG SKGIIIKGGH 190 200 210 220 230 240 SNDTDIAKDY LFTNEGVQTF ENERFKTKHT HGTGCTFSAV ITAELAKGRP LFEAVHKAKK 250 260 270 FISMSIQYTP EIGRGRGPVN HFAYLKKEGL DDELSK

References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Mu50 / ATCC 700699.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000017 Genomic DNA. Translation: BAB58255.1.
RefSeq	NP_372617.1. NC_002758.2.
3D structure databases
ProteinModelPortal	P66915.
ModBase	Search...
Protein-protein interaction databases
STRING	P66915.
2D gel databases
World-2DPAGE	0002:P66915.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000008171; EBSTAP00000007989; EBSTAG00000008170.
GeneID	1122110.
GenomeReviews	Gene locus SAV2093 in contig BA000017_GR.
KEGG	sav:SAV2093.
PATRIC	19565064. VBIStaAur52173_2167.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0351.
GeneTree	EBGT00050000024460.
HOGENOM	HBG476673.
OMA	FSAMRAY.
PhylomeDB	P66915.
ProtClustDB	PRK06427.
Enzyme and pathway databases
BioCyc	SAUR158878:SAV2093-MONOMER.
Family and domain databases
InterPro	IPR013749. HMP-P_kinase-1. IPR004399. HMP-P_kinase-2. [Graphical view]
KO	K00941.
Pfam	PF08543. Phos_pyr_kin. 1 hit. [Graphical view]
TIGRFAMs	TIGR00097. HMP-P_kinase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THID_STAAM

Accession

Primary (citable) accession number: P66915
Secondary accession number(s): Q99SG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program