P66876 (METB_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cystathionine gamma-synthase
Short name=CGS
EC=2.5.1.48

Alternative name(s):
O-succinylhomoserine (thiol)-lyase

Gene names

Name:	metB
Ordered Locus Names:	Mb1108

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cystathionine gamma-synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Cystathionine gamma-synthase		PRO_0000114763
Amino acid modifications
	Modified residue	208	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P66876 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: B6D9C0F78F3EAE71	FASTA	388	40,982
	10 20 30 40 50 60 MSEDRTGHQG ISGPATRAIH AGYRPDPATG AVNVPIYASS TFAQDGVGGL RGGFEYARTG 70 80 90 100 110 120 NPTRAALEAS LAAVEEGAFA RAFSSGMAAT DCALRAMLRP GDHVVIPDDA YGGTFRLIDK 130 140 150 160 170 180 VFTRWDVQYT PVRLADLDAV GAAITPRTRL IWVETPTNPL LSIADITAIA ELGTDRSAKV 190 200 210 220 230 240 LVDNTFASPA LQQPLRLGAD VVLHSTTKYI GGHSDVVGGA LVTNDEELDE EFAFLQNGAG 250 260 270 280 290 300 AVPGPFDAYL TMRGLKTLVL RMQRHSENAC AVAEFLADHP SVSSVLYPGL PSHPGHEIAA 310 320 330 340 350 360 RQMRGFGGMV SVRMRAGRRA AQDLCAKTRV FILAESLGGV ESLIEHPSAM THASTAGSQL 370 380 EVPDDLVRLS VGIEDIADLL GDLEQALG

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248337 Genomic DNA. Translation: CAD93968.1.
RefSeq	NP_854763.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P66876.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000014273; EBMYCP00000014108; EBMYCG00000014271.
GeneID	1090439.
GenomeReviews	Gene locus Mb1108 in contig BX248333_GR.
KEGG	mbo:Mb1108.
PATRIC	18004113. VBIMycBov88188_1209.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016651.
HOGENOM	HBG754729.
OMA	CETPTNP.
ProtClustDB	PRK07811.
Enzyme and pathway databases
BioCyc	MBOV233413:MB1108-MONOMER.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K01739.
PANTHER	PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METB_MYCBO

Accession

Primary (citable) accession number: P66876
Secondary accession number(s): O53427

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program