Reviewed, UniProtKB/Swiss-Prot P66867 (SUCC_BRUME)

Names and origin

Protein names

Recommended name:
    Succinyl-CoA ligase [ADP-forming] subunit beta
    EC=6.2.1.5
Alternative name(s):
    Succinyl-CoA synthetase subunit beta
      Short name=SCS-beta

Gene names

Name:	sucC
Ordered Locus Names:	BMEI0138

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + succinate + CoA = ADP + phosphate + succinyl-CoA. HAMAP MF_00558
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00558
Subunit structure	Heterotetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the succinate/malate CoA ligase beta subunit family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW succinate-CoA ligase (ADP-forming) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Succinyl-CoA ligase [ADP-forming] subunit beta HAMAP MF_00558		PRO_0000102820
Regions
	Domain	9 – 254	246	ATP-grasp
	Nucleotide binding	35 – 118	84	ATP By similarity
Sites
	Metal binding	207	1	Magnesium or manganese By similarity
	Metal binding	209	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P66867-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 2183A24659700A25	FASTA	398	42,527
	10 20 30 40 50 60 MNIHEYQAKR LLHTYGAPIA NGVAVYSVEQ AEEWAKTLPG PLYVVKSQIH AGGRGKGKFK 70 80 90 100 110 120 ELPADAKGGV RLAKSVEEVV ANAKEMLGNT LVTKQTGEAG KQVNRLYIED GADIERELYL 130 140 150 160 170 180 SILIDRSVGR PAFVVSTEGG MDIEAVAEET PEKIVTVAID PAKGVTDEDA NKLADALKLE 190 200 210 220 230 240 GGAREDGLKL FPILYKAFTE KDMSLLEINP LIVMTNGRVR VLDAKVSFDN NALFRHPDIV 250 260 270 280 290 300 ELRDLTEEDP KEIEASKYDL AYVALDGNIG CMVNGAGLAM ATMDIIKLYG AEPANFLDVG 310 320 330 340 350 360 GGASKEKVTA AFKIITADPA VEGILVNIFG GIMKCDVIAE GVIAAVKEVG LKVPLVVRLE 370 380 390 GTNVELGKKI INESGLNVIS ADDLDDAAQK IVAAVKGN

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL51320.1.
PIR	AE3269.
RefSeq	NP_539056.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1195850.
GenomeReviews	Gene locus BMEI0138 in contig AE008917_GR.
KEGG	bme:BMEI0138.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P66867.
OMA	QPYQARE
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0138-MON.
BRENDA	6.2.1.5. 277959.
Family and domain databases
HAMAP	MF_00558. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013650. ATP-grasp_succ-CoA_synth-type. IPR013816. ATP_grasp_subdomain_2. IPR005811. CoA_ligase. IPR017866. Succ-CoA_synthase_bsu_CS. IPR005809. Succ_CoA_synthase_bsu. IPR016102. Succinyl-CoA_synth-like. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.261. Succinyl-CoA_synth-like. 1 hit.
PANTHER	PTHR11815. CoA_lig_beta. 1 hit.
Pfam	PF08442. ATP-grasp_2. 1 hit. PF00549. Ligase_CoA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01016. sucCoAbeta. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS01217. SUCCINYL_COA_LIG_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SUCC_BRUME

Accession

Primary (citable) accession number: P66867
Secondary accession number(s): Q8YJE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 24, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)