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Protein

SsrA-binding protein

Gene

smpB

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SsrA-binding proteinUniRule annotation
Alternative name(s):
Small protein BUniRule annotation
Gene namesi
Name:smpBUniRule annotation
Ordered Locus Names:BMEI1300
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000419 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

  • Note: The tmRNA-SmpB complex associates with stalled 70S ribosomes.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 158158SsrA-binding proteinPRO_0000102918Add
BLAST

Proteomic databases

PRIDEiP66858.

Interactioni

Protein-protein interaction databases

STRINGi224914.BAWG_0913.

Structurei

3D structure databases

ProteinModelPortaliP66858.
SMRiP66858. Positions 11-132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SmpB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UH4. Bacteria.
COG0691. LUCA.
HOGENOMiHOG000009628.
KOiK03664.
OMAiFLLNAHI.
OrthoDBiEOG6HXJ9P.

Family and domain databases

Gene3Di2.40.280.10. 1 hit.
HAMAPiMF_00023. SmpB.
InterProiIPR023620. SmpB.
IPR000037. SsrA-bd_prot.
IPR020081. SsrA-bd_prot_CS.
[Graphical view]
PfamiPF01668. SmpB. 1 hit.
[Graphical view]
ProDomiPD004488. SmpB. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF74982. SSF74982. 1 hit.
TIGRFAMsiTIGR00086. smpB. 1 hit.
PROSITEiPS01317. SSRP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P66858-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKPKNSPAR KMIAENRKAR FNFEILDTLE AGLVLTGTEV KSLRANQANI
60 70 80 90 100
AESYASFEDG EFWLINSYIP EYTQGNRFNH EPRRLRKLLV SRREMSRLFN
110 120 130 140 150
SVSREGMTVV PLKLYFNDRG RAKLELALAR GKKTHDKRET EKKRDWNREK

ARLLRDRG
Length:158
Mass (Da):18,570
Last modified:October 11, 2004 - v1
Checksum:iF034DAD1DDD08D6E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52481.1.
PIRiAF3414.
RefSeqiWP_002963791.1. NZ_GG703778.1.

Genome annotation databases

EnsemblBacteriaiAAL52481; AAL52481; BMEI1300.
GeneIDi3788968.
KEGGibme:BMEI1300.
PATRICi17797365. VBIBruMel146950_0501.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52481.1.
PIRiAF3414.
RefSeqiWP_002963791.1. NZ_GG703778.1.

3D structure databases

ProteinModelPortaliP66858.
SMRiP66858. Positions 11-132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BAWG_0913.

Proteomic databases

PRIDEiP66858.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL52481; AAL52481; BMEI1300.
GeneIDi3788968.
KEGGibme:BMEI1300.
PATRICi17797365. VBIBruMel146950_0501.

Phylogenomic databases

eggNOGiENOG4108UH4. Bacteria.
COG0691. LUCA.
HOGENOMiHOG000009628.
KOiK03664.
OMAiFLLNAHI.
OrthoDBiEOG6HXJ9P.

Family and domain databases

Gene3Di2.40.280.10. 1 hit.
HAMAPiMF_00023. SmpB.
InterProiIPR023620. SmpB.
IPR000037. SsrA-bd_prot.
IPR020081. SsrA-bd_prot_CS.
[Graphical view]
PfamiPF01668. SmpB. 1 hit.
[Graphical view]
ProDomiPD004488. SmpB. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF74982. SSF74982. 1 hit.
TIGRFAMsiTIGR00086. smpB. 1 hit.
PROSITEiPS01317. SSRP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiSSRP_BRUME
AccessioniPrimary (citable) accession number: P66858
Secondary accession number(s): Q8YG61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 11, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.