P66835 (SPEE_STRPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermidine synthase
EC=2.5.1.16

Alternative name(s):
Putrescine aminopropyltransferase
Short name=PAPT
SPDSY

Gene names

Name:	speE
Ordered Locus Names:	SP_0918

Organism

Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) [Complete proteome] [HAMAP]

Taxonomic identifier

170187 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor By similarity. HAMAP-Rule MF_00198
Catalytic activity	S-adenosylmethioninamine + putrescine = S-methyl-5'-thioadenosine + spermidine. HAMAP-Rule MF_00198
Pathway	Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1. HAMAP-Rule MF_00198
Subunit structure	Homodimer or homotetramer By similarity. HAMAP-Rule MF_00198
Sequence similarities	Belongs to the spermidine/spermine synthase family.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Spermidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	spermidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	spermidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Spermidine synthase HAMAP-Rule MF_00198		PRO_0000156510
Regions
	Region	137 – 138	2	S-adenosylmethioninamine binding By similarity
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	31	1	S-adenosylmethioninamine By similarity
	Binding site	61	1	Putrescine By similarity
	Binding site	86	1	S-adenosylmethioninamine By similarity
	Binding site	106	1	S-adenosylmethioninamine By similarity
	Binding site	158	1	Putrescine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P66835 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 2131961004F6917F	FASTA	286	32,859
	10 20 30 40 50 60 MDLWFSEVHT PDVKLSLRTA KQLYAGKSEW QDIEVLDTPA FGKILILNGH VLFSDADDFV 70 80 90 100 110 120 YNEMTVHVPM AVHPNPKKVL VIGGGDGGVA QVLTLYPELE QIDIVEPDEM LVEVCREYFP 130 140 150 160 170 180 DFAAGLDDPR VTIYYQNGLR FLRNCEDDYD IIINDATDPF GHTEGLFTKE FYGNSYRALK 190 200 210 220 230 240 EDGIMIYQHG SPFFDEDESA CRSMHRKVNQ AFPISRVYQA HIPTSPAGYW LFGFASKKYH 250 260 270 280 PVKDFDKEGW KKRQLFTEYY TANLHVGAFM LPKYVEDILE EEEGKK

References

[1]

"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae." Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M. Science 293:498-506(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-334 / TIGR4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005672 Genomic DNA. Translation: AAK75042.1.
PIR	A95106.
RefSeq	NP_345402.1. NC_003028.3.
3D structure databases
ProteinModelPortal	P66835.
ModBase	Search...
Protein-protein interaction databases
STRING	170187.SP_0918.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK75042; AAK75042; SP_0918.
GeneID	930871.
KEGG	spn:SP_0918.
PATRIC	19706239. VBIStrPne105772_0964.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0421.
HOGENOM	HOG000256146.
KO	K00797.
OMA	HPTIVDG.
ProtClustDB	PRK00811.
Enzyme and pathway databases
BioCyc	SPNE170187:GHGN-922-MONOMER.
UniPathway	UPA00248; UER00314.
Family and domain databases
HAMAP	MF_00198. Spermidine_synth.
InterPro	IPR001045. Spermidine/spermine_synthase. [Graphical view]
PANTHER	PTHR11558. PTHR11558. 1 hit.
Pfam	PF01564. Spermine_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00417. speE. 1 hit.
PROSITE	PS01330. SPERMIDINE_SYNTHASE_1. False negative. PS51006. SPERMIDINE_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEE_STRPN

Accession

Primary (citable) accession number: P66835
Secondary accession number(s): Q97RA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 29, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program