Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DnaA initiator-associating protein DiaA

Gene

diaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the timely initiation of chromosomal replication via direct interactions with the DnaA initiator protein.2 Publications

GO - Molecular functioni

  • carbohydrate binding Source: UniProtKB-HAMAP
  • identical protein binding Source: IntAct

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciEcoCyc:YRAO-MONOMER.
ECOL316407:JW3118-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaA initiator-associating protein DiaA
Gene namesi
Name:diaA
Synonyms:yraO
Ordered Locus Names:b3149, JW3118
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12780. diaA.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52S → F: Abolishes stimulation of DnaA-dependent DNA synthesis. 1 Publication1
Mutagenesisi71R → Q: Reduces affinity for DnaA. Strongly reduces DnaA-dependent DNA synthesis. 1 Publication1
Mutagenesisi72P → A or S: Strongly reduces affinity for DnaA. Reduces DnaA-dependent DNA synthesis. 1 Publication1
Mutagenesisi83N → D: Abolishes association of homodimers into homotetramers. Reduces affinity for DnaA. Strongly reduces DnaA-dependent DNA synthesis. 1 Publication1
Mutagenesisi101K → E: Abolishes association of homodimers into homotetramers. Strongly reduces DnaA-dependent DNA synthesis. 1 Publication1
Mutagenesisi190L → A or P: Abolishes interaction with DnaA. Strongly reduces DnaA-dependent DNA synthesis. 1 Publication1
Mutagenesisi191F → L or S: Abolishes interaction with DnaA. Strongly reduces DnaA-dependent DNA synthesis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001365551 – 196DnaA initiator-associating protein DiaAAdd BLAST196

Proteomic databases

PaxDbiP66817.
PRIDEiP66817.

Interactioni

Subunit structurei

Homotetramer; dimer of dimers. May be in a rapid association-dissociation equilibrium between homodimers and homotetramers. Formation of homotetramers is required for stimulation of replication. Interacts with DnaA.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-1125806,EBI-1125806
dnaAP030045EBI-1125806,EBI-548951

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4261991. 138 interactors.
DIPiDIP-35981N.
IntActiP66817. 10 interactors.
STRINGi511145.b3149.

Structurei

Secondary structure

1196
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 22Combined sources21
Helixi24 – 39Combined sources16
Beta strandi44 – 49Combined sources6
Helixi51 – 64Combined sources14
Beta strandi67 – 69Combined sources3
Beta strandi76 – 80Combined sources5
Helixi83 – 89Combined sources7
Helixi95 – 97Combined sources3
Helixi98 – 106Combined sources9
Beta strandi112 – 116Combined sources5
Beta strandi118 – 120Combined sources3
Helixi123 – 134Combined sources12
Beta strandi138 – 143Combined sources6
Helixi148 – 151Combined sources4
Beta strandi158 – 162Combined sources5
Helixi168 – 190Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YVAX-ray1.85A/B1-196[»]
4U6NX-ray1.91A2-196[»]
ProteinModelPortaliP66817.
SMRiP66817.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP66817.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 196SISAdd BLAST163

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 191Important for interaction with DnaA2

Sequence similaritiesi

Belongs to the SIS family. DiaA subfamily.Curated
Contains 1 SIS domain.Curated

Phylogenomic databases

eggNOGiENOG4105F55. Bacteria.
COG0279. LUCA.
HOGENOMiHOG000237572.
InParanoidiP66817.
KOiK12961.
OMAiLHEEVYA.
PhylomeDBiP66817.

Family and domain databases

HAMAPiMF_01157. SIS_DiaA. 1 hit.
InterProiIPR023070. DiaA.
IPR001347. SIS.
[Graphical view]
PfamiPF13580. SIS_2. 1 hit.
[Graphical view]
PROSITEiPS51464. SIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P66817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQERIKACFT ESIQTQIAAA EALPDAISRA AMTLVQSLLN GNKILCCGNG
60 70 80 90 100
TSAANAQHFA ASMINRFETE RPSLPAIALN TDNVVLTAIA NDRLHDEVYA
110 120 130 140 150
KQVRALGHAG DVLLAISTRG NSRDIVKAVE AAVTRDMTIV ALTGYDGGEL
160 170 180 190
AGLLGPQDVE IRIPSHRSAR IQEMHMLTVN CLCDLIDNTL FPHQDD
Length:196
Mass (Da):21,106
Last modified:October 11, 2004 - v1
Checksum:iF9ADFE9C0DF365A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57952.1.
U00096 Genomic DNA. Translation: AAC76183.1.
AP009048 Genomic DNA. Translation: BAE77195.1.
PIRiA65105.
RefSeqiNP_417618.1. NC_000913.3.
WP_001158034.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76183; AAC76183; b3149.
BAE77195; BAE77195; BAE77195.
GeneIDi947661.
KEGGiecj:JW3118.
eco:b3149.
PATRICi32121716. VBIEscCol129921_3244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57952.1.
U00096 Genomic DNA. Translation: AAC76183.1.
AP009048 Genomic DNA. Translation: BAE77195.1.
PIRiA65105.
RefSeqiNP_417618.1. NC_000913.3.
WP_001158034.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YVAX-ray1.85A/B1-196[»]
4U6NX-ray1.91A2-196[»]
ProteinModelPortaliP66817.
SMRiP66817.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261991. 138 interactors.
DIPiDIP-35981N.
IntActiP66817. 10 interactors.
STRINGi511145.b3149.

Proteomic databases

PaxDbiP66817.
PRIDEiP66817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76183; AAC76183; b3149.
BAE77195; BAE77195; BAE77195.
GeneIDi947661.
KEGGiecj:JW3118.
eco:b3149.
PATRICi32121716. VBIEscCol129921_3244.

Organism-specific databases

EchoBASEiEB2633.
EcoGeneiEG12780. diaA.

Phylogenomic databases

eggNOGiENOG4105F55. Bacteria.
COG0279. LUCA.
HOGENOMiHOG000237572.
InParanoidiP66817.
KOiK12961.
OMAiLHEEVYA.
PhylomeDBiP66817.

Enzyme and pathway databases

BioCyciEcoCyc:YRAO-MONOMER.
ECOL316407:JW3118-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP66817.
PROiP66817.

Family and domain databases

HAMAPiMF_01157. SIS_DiaA. 1 hit.
InterProiIPR023070. DiaA.
IPR001347. SIS.
[Graphical view]
PfamiPF13580. SIS_2. 1 hit.
[Graphical view]
PROSITEiPS51464. SIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDIAA_ECOLI
AccessioniPrimary (citable) accession number: P66817
Secondary accession number(s): P45466, Q2M961
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.