Reviewed, UniProtKB/Swiss-Prot P66773 (SDHL_MYCTU)

Names and origin

Protein names

Recommended name:
    L-serine dehydratase
      Short name=SDH
    EC=4.3.1.17
Alternative name(s):
    L-serine deaminase
      Short name=L-SD

Gene names

Name:	sdaA
Ordered Locus Names:	Rv0069c, MT0075
ORF Names:	MTV030.12c

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-serine = pyruvate + NH3.
Cofactor	Binds 1 4Fe-4S cluster Probable.
Pathway	Carbohydrate biosynthesis; gluconeogenesis.
Sequence similarities	Belongs to the iron-sulfur dependent L-serine dehydratase family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW L-serine ammonia-lyase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	L-serine dehydratase		PRO_0000171909

Sequences

	Sequence		Length	Mass (Da)
	P66773-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 228C62DCE67FEAFD	FASTA	461	48,576
	10 20 30 40 50 60 MTISVFDLFT IGIGPSSSHT VGPMRAANQF VVALRRRGHL DDLEAMRVDL FGSLAATGAG 70 80 90 100 110 120 HGTMSAILLG LEGCQPETIT TEHKERRLAE IAASGVTRIG GVIPVPLTER DIDLHPDIVL 130 140 150 160 170 180 PTHPNGMTFT AAGPHGRVLA TETYFSVGGG FIVTEQTSGN SGQHPCSVAL PYVSAQELLD 190 200 210 220 230 240 ICDRLDVSIS EAALRNETCC RTENEVRAAL LHLRDVMVEC EQRSIAREGL LPGGLRVRRR 250 260 270 280 290 300 AKVWYDRLNA EDPTRKPEFA EDWVNLVALA VNEENASGGR VVTAPTNGAA GIVPAVLHYA 310 320 330 340 350 360 IHYTSAGAGD PDDVTVRFLL TAGAIGSLFK ERASISGAEV GCQGEVGSAA AMAAAGLAEI 370 380 390 400 410 420 LGGTPRQVEN AAEIAMEHSL GLTCDPIAGL VQIPCIERNA ISAGKAINAA RMALRGDGIH 430 440 450 460 RVTLDQVIDT MRATGADMHT KYKETSAGGL AINVAVNIVE C

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842572 Genomic DNA. Translation: CAA16250.1. AE000516 Genomic DNA. Translation: AAK44299.1.
PIR	F70848.
RefSeq	NP_214583.1. NP_334485.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	886986. 922809.
GenomeReviews	Gene locus MT0075 in contig AE000516_GR. Gene locus Rv0069c in contig AL123456_GR.
KEGG	mtc:MT0075. mtu:Rv0069c.
TIGR	MT0075.
Organism-specific databases
TubercuList	Rv0069c.
Phylogenomic databases
HOGENOM	P66773.
OMA	WAAMREC.
Enzyme and pathway databases
BRENDA	4.3.1.17. 809.
Family and domain databases
InterPro	IPR004644. Fe-S_L-Ser_mono. IPR005130. Ser_deHydtase_asu. IPR005131. Ser_deHydtase_bsu. [Graphical view]
Pfam	PF03313. SDH_alpha. 1 hit. PF03315. SDH_beta. 1 hit. [Graphical view]
TIGRFAMs	TIGR00720. sda_mono. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SDHL_MYCTU

Accession

Primary (citable) accession number: P66773
Secondary accession number(s): O53614

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)