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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8MagnesiumUniRule annotation1
Metal bindingi67MagnesiumUniRule annotation1
Metal bindingi139MagnesiumUniRule annotation1
Metal bindingi142MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciSENT99287:G1FZD-1917-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:STM1898
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001831311 – 173Crossover junction endodeoxyribonuclease RuvCAdd BLAST173

Proteomic databases

PaxDbiP66762.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM1898.

Structurei

3D structure databases

ProteinModelPortaliP66762.
SMRiP66762.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105NHU. Bacteria.
COG0817. LUCA.
HOGENOMiHOG000012181.
KOiK01159.
OMAiGLTRCGI.
PhylomeDBiP66762.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC. 1 hit.
InterProiView protein in InterPro
IPR012337. RNaseH-like_sf.
IPR036397. RNaseH_sf.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
PANTHERiPTHR30194. PTHR30194. 1 hit.
PfamiView protein in Pfam
PF02075. RuvC. 1 hit.
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiView protein in PROSITE
PS01321. RUVC. 1 hit.

Sequencei

Sequence statusi: Complete.

P66762-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIILGIDPG SRITGYGVIR QVGRQLTYLG SGCIRTKVDD LPSRLKLIYA
60 70 80 90 100
GVTEIITQFQ PDYFAIEQVF MAKNADSALK LGQARGVAIV AAVNQELPVF
110 120 130 140 150
EYAARQVKQT VVGIGSAEKS QVQHMVRTLL KLPANPQADA ADALAIAITH
160 170
CHVSQNAMQM SESRLNLARG RLR
Length:173
Mass (Da):18,791
Last modified:October 11, 2004 - v1
Checksum:i41FCC3391A68AF62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA. Translation: AAL20814.1.
RefSeqiNP_460855.1. NC_003197.2.
WP_000022509.1. NC_003197.2.

Genome annotation databases

EnsemblBacteriaiAAL20814; AAL20814; STM1898.
GeneIDi1253419.
31658484.
KEGGistm:STM1898.
PATRICifig|99287.12.peg.2013.

Similar proteinsi

Entry informationi

Entry nameiRUVC_SALTY
AccessioniPrimary (citable) accession number: P66762
Secondary accession number(s): Q8XGF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: February 28, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome