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P66761

- RUVC_MYCBO

UniProt

P66761 - RUVC_MYCBO

Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity.By similarity

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi7 – 71MagnesiumBy similarity
    Metal bindingi68 – 681MagnesiumBy similarity
    Metal bindingi141 – 1411MagnesiumBy similarity
    Metal bindingi144 – 1441MagnesiumBy similarity

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-HAMAP
    2. DNA repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
    Alternative name(s):
    Holliday junction nuclease RuvC
    Holliday junction resolvase RuvC
    Gene namesi
    Name:ruvC
    Ordered Locus Names:Mb2625c
    OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
    Taxonomic identifieri233413 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001419: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 188188Crossover junction endodeoxyribonuclease RuvCPRO_0000183111Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi233413.Mb2625c.

    Structurei

    3D structure databases

    ProteinModelPortaliP66761.
    SMRiP66761. Positions 3-137.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuvC family.Curated

    Phylogenomic databases

    eggNOGiCOG0817.
    HOGENOMiHOG000012182.
    KOiK01159.
    OMAiCHAWRGG.
    OrthoDBiEOG6RG044.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    HAMAPiMF_00034. RuvC.
    InterProiIPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view]
    PfamiPF02075. RuvC. 1 hit.
    [Graphical view]
    PRINTSiPR00696. RSOLVASERUVC.
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00228. ruvC. 1 hit.
    PROSITEiPS01321. RUVC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P66761-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVMGVDPGL TRCGLSLIES GRGRQLTALD VDVVRTPSDA ALAQRLLAIS    50
    DAVEHWLDTH HPEVVAIERV FSQLNVTTVM GTAQAGGVIA LAAAKRGVDV 100
    HFHTPSEVKA AVTGNGSADK AQVTAMVTKI LALQAKPTPA DAADALALAI 150
    CHCWRAPTIA RMAEATSRAE ARAAQQRHAY LAKLKAAR 188
    Length:188
    Mass (Da):19,786
    Last modified:October 11, 2004 - v1
    Checksum:i1D8619C9150406FB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO43889.1.
    RefSeqiNP_856271.1. NC_002945.3.

    Genome annotation databases

    EnsemblBacteriaiCAD94810; CAD94810; Mb2625c.
    GeneIDi1092007.
    KEGGimbo:Mb2625c.
    PATRICi18007499. VBIMycBov88188_2886.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO43889.1 .
    RefSeqi NP_856271.1. NC_002945.3.

    3D structure databases

    ProteinModelPortali P66761.
    SMRi P66761. Positions 3-137.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 233413.Mb2625c.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD94810 ; CAD94810 ; Mb2625c .
    GeneIDi 1092007.
    KEGGi mbo:Mb2625c.
    PATRICi 18007499. VBIMycBov88188_2886.

    Phylogenomic databases

    eggNOGi COG0817.
    HOGENOMi HOG000012182.
    KOi K01159.
    OMAi CHAWRGG.
    OrthoDBi EOG6RG044.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    HAMAPi MF_00034. RuvC.
    InterProi IPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view ]
    Pfami PF02075. RuvC. 1 hit.
    [Graphical view ]
    PRINTSi PR00696. RSOLVASERUVC.
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00228. ruvC. 1 hit.
    PROSITEi PS01321. RUVC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-935 / AF2122/97.

    Entry informationi

    Entry nameiRUVC_MYCBO
    AccessioniPrimary (citable) accession number: P66761
    Secondary accession number(s): Q50627, X2BL50
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3