ID RUVA_BRUME Reviewed; 205 AA. AC P66742; Q8YIV6; DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot. DT 11-OCT-2004, sequence version 1. DT 27-MAR-2024, entry version 103. DE RecName: Full=Holliday junction branch migration complex subunit RuvA {ECO:0000255|HAMAP-Rule:MF_00031}; GN Name=ruvA {ECO:0000255|HAMAP-Rule:MF_00031}; GN OrderedLocusNames=BMEI0333; OS Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094). OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales; OC Brucellaceae; Brucella/Ochrobactrum group; Brucella. OX NCBI_TaxID=224914; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=16M / ATCC 23456 / NCTC 10094; RX PubMed=11756688; DOI=10.1073/pnas.221575398; RA DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., RA Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., RA Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., RA Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., RA Haselkorn R., Kyrpides N.C., Overbeek R.; RT "The genome sequence of the facultative intracellular pathogen Brucella RT melitensis."; RL Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002). CC -!- FUNCTION: The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) CC DNA during genetic recombination and DNA repair, while the RuvA-RuvB CC complex plays an important role in the rescue of blocked DNA CC replication forks via replication fork reversal (RFR). RuvA CC specifically binds to HJ cruciform DNA, conferring on it an open CC structure. The RuvB hexamer acts as an ATP-dependent pump, pulling CC dsDNA into and through the RuvAB complex. HJ branch migration allows CC RuvC to scan DNA until it finds its consensus sequence, where it CC cleaves and resolves the cruciform DNA. {ECO:0000255|HAMAP- CC Rule:MF_00031}. CC -!- SUBUNIT: Homotetramer. Forms an RuvA(8)-RuvB(12)-Holliday junction (HJ) CC complex. HJ DNA is sandwiched between 2 RuvA tetramers; dsDNA enters CC through RuvA and exits via RuvB. An RuvB hexamer assembles on each DNA CC strand where it exits the tetramer. Each RuvB hexamer is contacted by CC two RuvA subunits (via domain III) on 2 adjacent RuvB subunits; this CC complex drives branch migration. In the full resolvosome a probable CC DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ. CC {ECO:0000255|HAMAP-Rule:MF_00031}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00031}. CC -!- DOMAIN: Has three domains with a flexible linker between the domains II CC and III and assumes an 'L' shape. Domain III is highly mobile and CC contacts RuvB. {ECO:0000255|HAMAP-Rule:MF_00031}. CC -!- SIMILARITY: Belongs to the RuvA family. {ECO:0000255|HAMAP- CC Rule:MF_00031}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE008917; AAL51514.1; -; Genomic_DNA. DR PIR; AG3293; AG3293. DR RefSeq; WP_002964792.1; NZ_GG703781.1. DR AlphaFoldDB; P66742; -. DR SMR; P66742; -. DR GeneID; 58775287; -. DR KEGG; bme:BMEI0333; -. DR KEGG; bmel:DK63_1101; -. DR PATRIC; fig|224914.52.peg.1159; -. DR eggNOG; COG0632; Bacteria. DR PhylomeDB; P66742; -. DR Proteomes; UP000000419; Chromosome I. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0009379; C:Holliday junction helicase complex; IEA:InterPro. DR GO; GO:0048476; C:Holliday junction resolvase complex; IEA:UniProtKB-UniRule. DR GO; GO:0005524; F:ATP binding; IEA:InterPro. DR GO; GO:0000400; F:four-way junction DNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0009378; F:four-way junction helicase activity; IEA:InterPro. DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule. DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule. DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1. DR Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1. DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1. DR HAMAP; MF_00031; DNA_HJ_migration_RuvA; 1. DR InterPro; IPR013849; DNA_helicase_Holl-junc_RuvA_I. DR InterPro; IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR000085; RuvA. DR InterPro; IPR010994; RuvA_2-like. DR InterPro; IPR011114; RuvA_C. DR InterPro; IPR036267; RuvA_C_sf. DR NCBIfam; TIGR00084; ruvA; 1. DR PANTHER; PTHR33796; HOLLIDAY JUNCTION ATP-DEPENDENT DNA HELICASE RUVA; 1. DR PANTHER; PTHR33796:SF1; HOLLIDAY JUNCTION ATP-DEPENDENT DNA HELICASE RUVA; 1. DR Pfam; PF14520; HHH_5; 1. DR Pfam; PF07499; RuvA_C; 1. DR Pfam; PF01330; RuvA_N; 1. DR SMART; SM00278; HhH1; 2. DR SUPFAM; SSF46929; DNA helicase RuvA subunit, C-terminal domain; 1. DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1. DR SUPFAM; SSF47781; RuvA domain 2-like; 1. PE 3: Inferred from homology; KW Cytoplasm; DNA damage; DNA recombination; DNA repair; DNA-binding. FT CHAIN 1..205 FT /note="Holliday junction branch migration complex subunit FT RuvA" FT /id="PRO_0000094610" FT REGION 1..64 FT /note="Domain I" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00031" FT REGION 65..143 FT /note="Domain II" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00031" FT REGION 144..152 FT /note="Flexible linker" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00031" FT REGION 153..205 FT /note="Domain III" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00031" SQ SEQUENCE 205 AA; 21227 MW; 6BC6165956AAAF25 CRC64; MIGKLKGVID EIAEDHAVID VHGVGYVAFC SARTLGNLGG AGEAAILFIE TYVREDMIRL YGFATQLERE WFRLLQNVQG VGAKVALAVL GTLSPSELAN AIALRDIAMV SRAPGVGKKV AERIVTELKN KAPAFAGEAS GTIGLKQELG AGAAPAPVAD AVSALSNLGY SRDQAANAVA AALKETGEGA DSAKLIRLGL KELSQ //