P66742 (RUVA_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Holliday junction ATP-dependent DNA helicase RuvA
EC=3.6.4.12

Gene names

Name:	ruvA
Ordered Locus Names:	BMEI0333

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB By similarity. HAMAP-Rule MF_00031
Catalytic activity	ATP + H2O = ADP + phosphate. HAMAP-Rule MF_00031
Subunit structure	Forms a complex with RuvB By similarity.
Sequence similarities	Belongs to the RuvA family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA duplex unwinding Inferred from electronic annotation. Source: GOC DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP
Cellular_component	Holliday junction helicase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW four-way junction helicase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	Holliday junction ATP-dependent DNA helicase RuvA HAMAP-Rule MF_00031		PRO_0000094610

Sequences

	Sequence		Length	Mass (Da)
	P66742 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 6BC6165956AAAF25	FASTA	205	21,227
	10 20 30 40 50 60 MIGKLKGVID EIAEDHAVID VHGVGYVAFC SARTLGNLGG AGEAAILFIE TYVREDMIRL 70 80 90 100 110 120 YGFATQLERE WFRLLQNVQG VGAKVALAVL GTLSPSELAN AIALRDIAMV SRAPGVGKKV 130 140 150 160 170 180 AERIVTELKN KAPAFAGEAS GTIGLKQELG AGAAPAPVAD AVSALSNLGY SRDQAANAVA 190 200 AALKETGEGA DSAKLIRLGL KELSQ

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51514.1.
PIR	AG3293.
RefSeq	NP_539250.1. NC_003317.1.
3D structure databases
ProteinModelPortal	P66742.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI0333.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL51514; AAL51514; BMEI0333.
GeneID	1196044.
KEGG	bme:BMEI0333.
PATRIC	17802317. VBIBruMel146950_3475.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000057115.
KO	K03550.
OMA	ALEREWF.
ProtClustDB	PRK00116.
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
HAMAP	MF_00031. DNA_helic_RuvA.
InterPro	IPR011114. DNA_helicas_Holl-junc_RuvA_C. IPR013849. DNA_helicase_Holl-junc_RuvA_I. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR012340. NA-bd_OB-fold. IPR000085. RuvA. IPR010994. RuvA_2-like. [Graphical view]
Pfam	PF07499. RuvA_C. 1 hit. PF01330. RuvA_N. 1 hit. [Graphical view]
SMART	SM00278. HhH1. 2 hits. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF47781. RuvA_2_like. 1 hit. SSF46929. RuvA_C-like. 1 hit.
TIGRFAMs	TIGR00084. ruvA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUVA_BRUME

Accession

Primary (citable) accession number: P66742
Secondary accession number(s): Q8YIV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program