Reviewed, UniProtKB/Swiss-Prot P66742 (RUVA_BRUME)

Names and origin

Protein names

Recommended name:
    Holliday junction ATP-dependent DNA helicase ruvA
    EC=3.6.1.-

Gene names

Name:	ruvA
Ordered Locus Names:	BMEI0333

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of ruvB By similarity.
Subunit structure	Forms a complex with ruvB. HAMAP MF_00031
Sequence similarities	Belongs to the ruvA family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP
Cellular component	Holliday junction helicase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW four-way junction helicase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	Holliday junction ATP-dependent DNA helicase ruvA HAMAP MF_00031		PRO_0000094610

Sequences

	Sequence		Length	Mass (Da)
	P66742-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 6BC6165956AAAF25	FASTA	205	21,227
	10 20 30 40 50 60 MIGKLKGVID EIAEDHAVID VHGVGYVAFC SARTLGNLGG AGEAAILFIE TYVREDMIRL 70 80 90 100 110 120 YGFATQLERE WFRLLQNVQG VGAKVALAVL GTLSPSELAN AIALRDIAMV SRAPGVGKKV 130 140 150 160 170 180 AERIVTELKN KAPAFAGEAS GTIGLKQELG AGAAPAPVAD AVSALSNLGY SRDQAANAVA 190 200 AALKETGEGA DSAKLIRLGL KELSQ

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL51514.1.
PIR	AG3293.
RefSeq	NP_539250.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1196044.
GenomeReviews	Gene locus BMEI0333 in contig AE008917_GR.
KEGG	bme:BMEI0333.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P66742.
OMA	DCHGVGY
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI0333-MON.
Family and domain databases
HAMAP	MF_00031. [Tree]
InterPro	IPR011114. DNA_helicas_Holl-junc_RuvA_C. IPR013849. DNA_helicase_Holl-junc_RuvA_I. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR000085. RuvA. IPR010994. RuvA_2-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF07499. RuvA_C. 1 hit. PF01330. RuvA_N. 1 hit. [Graphical view]
SMART	SM00278. HhH1. 2 hits. [Graphical view]
TIGRFAMs	TIGR00084. ruvA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUVA_BRUME

Accession

Primary (citable) accession number: P66742
Secondary accession number(s): Q8YIV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 24, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)