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P66679 (RNPH_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease PH

Short name=RNase PH
EC=2.7.7.56
Alternative name(s):
tRNA nucleotidyltransferase
Gene names
Name:rph
Ordered Locus Names:c4467
OrganismEscherichia coli O6 [Complete proteome] [HAMAP]
Taxonomic identifier217992 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length238 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates. Also acts to regulate the attenuation of pyrE By similarity. HAMAP MF_00564

Catalytic activity

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564

Sequence similarities

Belongs to the RNase PH family.

Sequence caution

The sequence AAN82903.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 238238Ribonuclease PH HAMAP MF_00564
PRO_0000139890

Sequences

Sequence LengthMass (Da)Tools
P66679 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: D5B174F3ECC8560E

FASTA23825,312
        10         20         30         40         50         60 
MRPAGRSNNQ VRPVTLTRNY TKHAEGSVLV EFGDTKVLCT ASIEEGVPRF LKGQGQGWIT 

        70         80         90        100        110        120 
AEYGMLPRST HTRNAREAAK GKQGGRTMEI QRLIARALRA AVDLKALGEF TITLDCDVLQ 

       130        140        150        160        170        180 
ADGGTRTASI TGACVALADA LQKLVENGKL KTNPMKGMVA AVSVGIVNGE AVCDLEYVED 

       190        200        210        220        230 
SAAETDMNVV MTEDGRIIEV QGTAEGEPFT HEELLTLLAL ARGGIESIVA TQKAALAN 

« Hide

References

[1]"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli."
Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R.
Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014075 Genomic DNA. Translation: AAN82903.1. Different initiation.
RefSeqNP_756329.2. NC_004431.1.

3D structure databases

ProteinModelPortalP66679.
SMRP66679. Positions 2-237.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000041349; EBESCP00000039698; EBESCG00000040399.
GeneID1038263.
GenomeReviewsGene locus c4467 in contig AE014075_GR.
KEGGecc:c4467.
PATRIC18286635. VBIEscCol75197_4192.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000011343.
HOGENOMHBG737187.
OMAMLPRATG.
ProtClustDBPRK00173.

Family and domain databases

HAMAPMF_00564. RNase_PH.
[Tree]
InterProIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
KOK00989.
PfamPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMSSF55666. 3_ExoRNase. 1 hit.
SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMsTIGR01966. RNasePH. 1 hit.
PROSITEPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNPH_ECOL6
AccessionPrimary (citable) accession number: P66679
Secondary accession number(s): Q8XD98
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families