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P66673

- RNH_BRUME

UniProt

P66673 - RNH_BRUME

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Protein

Ribonuclease HI

Gene

rnhA

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg(2+) ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1UniRule annotation
Metal bindingi9 – 91Magnesium 2UniRule annotation
Metal bindingi47 – 471Magnesium 1UniRule annotation
Metal bindingi69 – 691Magnesium 1UniRule annotation
Metal bindingi133 – 1331Magnesium 2UniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. nucleic acid binding Source: InterPro
  3. RNA-DNA hybrid ribonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease HIUniRule annotation (EC:3.1.26.4UniRule annotation)
Short name:
RNase HIUniRule annotation
Gene namesi
Name:rnhAUniRule annotation
Ordered Locus Names:BMEI1457
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 154154Ribonuclease HIPRO_0000195365Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224914.BMEI1457.

Structurei

3D structure databases

ProteinModelPortaliP66673.
SMRiP66673. Positions 1-139.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 141141RNase HUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RNase H family.UniRule annotation
Contains 1 RNase H domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000040465.
KOiK03469.
OMAiDSAYVKN.
OrthoDBiEOG696BTR.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H.
InterProiIPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P66673-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRIEAYTDG ACSGNPGPGG WGALLRWNGN EKELKGGEAE TTNNRMELMA
60 70 80 90 100
AISALSALKE PCEVDLYTDS VYVRDGISGW IEGWKRNGWK TAAKKPVKNA
110 120 130 140 150
ELWQALDEAR KAHKVTWHWI KGHAGHPENE RADELARAGM EPFKYAGHRT

LKVK
Length:154
Mass (Da):17,172
Last modified:October 11, 2004 - v1
Checksum:i3B76C03F52E01012
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52638.1.
PIRiAC3434.
RefSeqiNP_540374.1. NC_003317.1.

Genome annotation databases

EnsemblBacteriaiAAL52638; AAL52638; BMEI1457.
GeneIDi1197168.
KEGGibme:BMEI1457.
PATRICi17797045. VBIBruMel146950_0342.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52638.1 .
PIRi AC3434.
RefSeqi NP_540374.1. NC_003317.1.

3D structure databases

ProteinModelPortali P66673.
SMRi P66673. Positions 1-139.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224914.BMEI1457.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL52638 ; AAL52638 ; BMEI1457 .
GeneIDi 1197168.
KEGGi bme:BMEI1457.
PATRICi 17797045. VBIBruMel146950_0342.

Phylogenomic databases

HOGENOMi HOG000040465.
KOi K03469.
OMAi DSAYVKN.
OrthoDBi EOG696BTR.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
HAMAPi MF_00042. RNase_H.
InterProi IPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view ]
Pfami PF00075. RNase_H. 1 hit.
[Graphical view ]
SUPFAMi SSF53098. SSF53098. 1 hit.
PROSITEi PS50879. RNASE_H. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiRNH_BRUME
AccessioniPrimary (citable) accession number: P66673
Secondary accession number(s): Q8YFR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 26, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3