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P66673 (RNH_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease HI

Short name=RNase HI
EC=3.1.26.4
Gene names
Name:rnhA
Ordered Locus Names:BMEI1457
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length154 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP-Rule MF_00042

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00042

Cofactor

Binds 1 magnesium ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding By similarity.

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00042

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00042.

Sequence similarities

Belongs to the RNase H family.

Contains 1 RNase H domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA catabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA-DNA hybrid ribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 154154Ribonuclease HI HAMAP-Rule MF_00042
PRO_0000195365

Regions

Domain1 – 141141RNase H

Sites

Metal binding91Magnesium 1 By similarity
Metal binding91Magnesium 2 By similarity
Metal binding471Magnesium 1 By similarity
Metal binding691Magnesium 1 By similarity
Metal binding1331Magnesium 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P66673 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 3B76C03F52E01012

FASTA15417,172
        10         20         30         40         50         60 
MKRIEAYTDG ACSGNPGPGG WGALLRWNGN EKELKGGEAE TTNNRMELMA AISALSALKE 

        70         80         90        100        110        120 
PCEVDLYTDS VYVRDGISGW IEGWKRNGWK TAAKKPVKNA ELWQALDEAR KAHKVTWHWI 

       130        140        150 
KGHAGHPENE RADELARAGM EPFKYAGHRT LKVK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL52638.1.
PIRAC3434.
RefSeqNP_540374.1. NC_003317.1.

3D structure databases

ProteinModelPortalP66673.
SMRP66673. Positions 1-139.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI1457.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL52638; AAL52638; BMEI1457.
GeneID1197168.
KEGGbme:BMEI1457.
PATRIC17797045. VBIBruMel146950_0342.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000040465.
KOK03469.
OMADSAYVKN.
OrthoDBEOG696BTR.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00042. RNase_H.
InterProIPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMSSF53098. SSF53098. 1 hit.
PROSITEPS50879. RNASE_H. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNH_BRUME
AccessionPrimary (citable) accession number: P66673
Secondary accession number(s): Q8YFR3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names