Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P66673

- RNH_BRUME

UniProt

P66673 - RNH_BRUME

Protein

Ribonuclease HI

Gene

rnhA

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.UniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91Magnesium 1UniRule annotation
    Metal bindingi9 – 91Magnesium 2UniRule annotation
    Metal bindingi47 – 471Magnesium 1UniRule annotation
    Metal bindingi69 – 691Magnesium 1UniRule annotation
    Metal bindingi133 – 1331Magnesium 2UniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. nucleic acid binding Source: InterPro
    3. RNA-DNA hybrid ribonuclease activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. RNA catabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease HIUniRule annotation (EC:3.1.26.4UniRule annotation)
    Short name:
    RNase HIUniRule annotation
    Gene namesi
    Name:rnhAUniRule annotation
    Ordered Locus Names:BMEI1457
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 154154Ribonuclease HIPRO_0000195365Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224914.BMEI1457.

    Structurei

    3D structure databases

    ProteinModelPortaliP66673.
    SMRiP66673. Positions 1-139.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 141141RNase HUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RNase H family.UniRule annotation
    Contains 1 RNase H domain.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000040465.
    KOiK03469.
    OMAiDSAYVKN.
    OrthoDBiEOG696BTR.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    HAMAPiMF_00042. RNase_H.
    InterProiIPR022892. RNaseH.
    IPR012337. RNaseH-like_dom.
    IPR002156. RNaseH_domain.
    [Graphical view]
    PfamiPF00075. RNase_H. 1 hit.
    [Graphical view]
    SUPFAMiSSF53098. SSF53098. 1 hit.
    PROSITEiPS50879. RNASE_H. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P66673-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRIEAYTDG ACSGNPGPGG WGALLRWNGN EKELKGGEAE TTNNRMELMA    50
    AISALSALKE PCEVDLYTDS VYVRDGISGW IEGWKRNGWK TAAKKPVKNA 100
    ELWQALDEAR KAHKVTWHWI KGHAGHPENE RADELARAGM EPFKYAGHRT 150
    LKVK 154
    Length:154
    Mass (Da):17,172
    Last modified:October 11, 2004 - v1
    Checksum:i3B76C03F52E01012
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52638.1.
    PIRiAC3434.
    RefSeqiNP_540374.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL52638; AAL52638; BMEI1457.
    GeneIDi1197168.
    KEGGibme:BMEI1457.
    PATRICi17797045. VBIBruMel146950_0342.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL52638.1 .
    PIRi AC3434.
    RefSeqi NP_540374.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali P66673.
    SMRi P66673. Positions 1-139.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI1457.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL52638 ; AAL52638 ; BMEI1457 .
    GeneIDi 1197168.
    KEGGi bme:BMEI1457.
    PATRICi 17797045. VBIBruMel146950_0342.

    Phylogenomic databases

    HOGENOMi HOG000040465.
    KOi K03469.
    OMAi DSAYVKN.
    OrthoDBi EOG696BTR.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    HAMAPi MF_00042. RNase_H.
    InterProi IPR022892. RNaseH.
    IPR012337. RNaseH-like_dom.
    IPR002156. RNaseH_domain.
    [Graphical view ]
    Pfami PF00075. RNase_H. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53098. SSF53098. 1 hit.
    PROSITEi PS50879. RNASE_H. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiRNH_BRUME
    AccessioniPrimary (citable) accession number: P66673
    Secondary accession number(s): Q8YFR3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3