P66673 (RNH_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HI
Short name=RNase HI
EC=3.1.26.4

Gene names

Name:	rnhA
Ordered Locus Names:	BMEI1457

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	154 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP-Rule MF_00042
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00042
Cofactor	Binds 1 magnesium ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00042.
Sequence similarities	Belongs to the RNase H family. Contains 1 RNase H domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA catabolic process Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro ribonuclease H activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 154	154	Ribonuclease HI HAMAP-Rule MF_00042		PRO_0000195365
Regions
	Domain	1 – 141	141	RNase H
Sites
	Metal binding	9	1	Magnesium 1 By similarity
	Metal binding	9	1	Magnesium 2 By similarity
	Metal binding	47	1	Magnesium 1 By similarity
	Metal binding	69	1	Magnesium 1 By similarity
	Metal binding	133	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P66673 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 3B76C03F52E01012	FASTA	154	17,172
	10 20 30 40 50 60 MKRIEAYTDG ACSGNPGPGG WGALLRWNGN EKELKGGEAE TTNNRMELMA AISALSALKE 70 80 90 100 110 120 PCEVDLYTDS VYVRDGISGW IEGWKRNGWK TAAKKPVKNA ELWQALDEAR KAHKVTWHWI 130 140 150 KGHAGHPENE RADELARAGM EPFKYAGHRT LKVK

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52638.1.
PIR	AC3434.
RefSeq	NP_540374.1. NC_003317.1.
3D structure databases
ProteinModelPortal	P66673.
SMR	P66673. Positions 1-139.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI1457.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL52638; AAL52638; BMEI1457.
GeneID	1197168.
KEGG	bme:BMEI1457.
PATRIC	17797045. VBIBruMel146950_0342.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000040465.
KO	K03469.
OMA	MQEIEIF.
ProtClustDB	PRK00203.
Family and domain databases
HAMAP	MF_00042. RNase_H.
InterPro	IPR022892. RNaseH. IPR012337. RNaseH-like_dom. IPR002156. RNaseH_domain. [Graphical view]
Pfam	PF00075. RNase_H. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
PROSITE	PS50879. RNASE_H. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNH_BRUME

Accession

Primary (citable) accession number: P66673
Secondary accession number(s): Q8YFR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program