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Protein

Ribonuclease HI

Gene

rnhA

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1UniRule annotation
Metal bindingi9 – 91Magnesium 2UniRule annotation
Metal bindingi47 – 471Magnesium 1UniRule annotation
Metal bindingi69 – 691Magnesium 1UniRule annotation
Metal bindingi133 – 1331Magnesium 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease HIUniRule annotation (EC:3.1.26.4UniRule annotation)
Short name:
RNase HIUniRule annotation
Gene namesi
Name:rnhAUniRule annotation
Ordered Locus Names:BMEI1457
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 154154Ribonuclease HIPRO_0000195365Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224914.BAWG_0766.

Structurei

3D structure databases

ProteinModelPortaliP66673.
SMRiP66673. Positions 1-139.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 141141RNase HUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RNase H family.UniRule annotation
Contains 1 RNase H domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000040465.
KOiK03469.
OMAiITSWIHN.
OrthoDBiEOG696BTR.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H.
InterProiIPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P66673-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRIEAYTDG ACSGNPGPGG WGALLRWNGN EKELKGGEAE TTNNRMELMA
60 70 80 90 100
AISALSALKE PCEVDLYTDS VYVRDGISGW IEGWKRNGWK TAAKKPVKNA
110 120 130 140 150
ELWQALDEAR KAHKVTWHWI KGHAGHPENE RADELARAGM EPFKYAGHRT

LKVK
Length:154
Mass (Da):17,172
Last modified:October 11, 2004 - v1
Checksum:i3B76C03F52E01012
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52638.1.
PIRiAC3434.
RefSeqiNP_540374.1. NC_003317.1.
WP_002963635.1. NZ_GG703778.1.

Genome annotation databases

EnsemblBacteriaiAAL52638; AAL52638; BMEI1457.
GeneIDi3788906.
KEGGibme:BMEI1457.
PATRICi17797045. VBIBruMel146950_0342.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52638.1.
PIRiAC3434.
RefSeqiNP_540374.1. NC_003317.1.
WP_002963635.1. NZ_GG703778.1.

3D structure databases

ProteinModelPortaliP66673.
SMRiP66673. Positions 1-139.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BAWG_0766.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL52638; AAL52638; BMEI1457.
GeneIDi3788906.
KEGGibme:BMEI1457.
PATRICi17797045. VBIBruMel146950_0342.

Phylogenomic databases

HOGENOMiHOG000040465.
KOiK03469.
OMAiITSWIHN.
OrthoDBiEOG696BTR.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H.
InterProiIPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiRNH_BRUME
AccessioniPrimary (citable) accession number: P66673
Secondary accession number(s): Q8YFR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: June 24, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.