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Protein

Ribonuclease 3

Gene

rnc

Organism
Streptococcus pyogenes serotype M1
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon (By similarity).By similarity
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In this organism endogenous ribonuclease 3 and Cas9 are required for correct coprocessing of pre-crRNA and the trans-encoded small RNA (tracrRNA). Cas9, crRNA and tracrRNA are required for cleavage of invading DNA (PubMed:21455174, PubMed:24270795).2 Publications

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47MagnesiumUniRule annotation1
Active sitei51UniRule annotation1
Metal bindingi120MagnesiumUniRule annotation1
Active sitei123UniRule annotation1
Metal bindingi123MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease, RNA-binding, rRNA-binding
Biological processAntiviral defense, mRNA processing, rRNA processing, tRNA processing
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3UniRule annotation (EC:3.1.26.3UniRule annotation)
Alternative name(s):
Ribonuclease IIIUniRule annotation
Short name:
RNase IIIUniRule annotation
Gene namesi
Name:rncUniRule annotation
Synonyms:acpA
Ordered Locus Names:SPy_0531, M5005_Spy0438
OrganismiStreptococcus pyogenes serotype M1
Taxonomic identifieri301447 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000750 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Loss of correct coprocessing of pre-crRNA and tracrRNA. Loss of immunity against a plasmid with homology to CRISPR spacer sequences.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51D → A: No processing of pre-crRNA. 1 Publication1
Mutagenesisi154 – 230Missing : No processing of pre-crRNA. 1 PublicationAdd BLAST77

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001804431 – 230Ribonuclease 3Add BLAST230

Proteomic databases

PaxDbiP66670
PRIDEiP66670

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi160490.SPy_0531

Structurei

3D structure databases

ProteinModelPortaliP66670
SMRiP66670
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 134RNase IIIUniRule annotationAdd BLAST134
Domaini160 – 229DRBMUniRule annotationAdd BLAST70

Sequence similaritiesi

Belongs to the ribonuclease III family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108ZBM Bacteria
COG0571 LUCA
HOGENOMiHOG000246808
KOiK03685
OMAiLTHKSCK

Family and domain databases

CDDicd00593 RIBOc, 1 hit
Gene3Di1.10.1520.10, 1 hit
HAMAPiMF_00104 RNase_III, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR011907 RNase_III
IPR000999 RNase_III_dom
IPR036389 RNase_III_sf
PfamiView protein in Pfam
PF00035 dsrm, 1 hit
PF14622 Ribonucleas_3_3, 1 hit
SMARTiView protein in SMART
SM00358 DSRM, 1 hit
SM00535 RIBOc, 1 hit
SUPFAMiSSF69065 SSF69065, 1 hit
TIGRFAMsiTIGR02191 RNaseIII, 1 hit
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 1 hit
PS00517 RNASE_3_1, 1 hit
PS50142 RNASE_3_2, 1 hit

Sequencei

Sequence statusi: Complete.

P66670-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQLEELLST SFDIQFNDLT LLETAFTHTS YANEHRLLNV SHNERLEFLG
60 70 80 90 100
DAVLQLIISE YLFAKYPKKT EGDMSKLRSM IVREESLAGF SRFCSFDAYI
110 120 130 140 150
KLGKGEEKSG GRRRDTILGD LFEAFLGALL LDKGIDAVRR FLKQVMIPQV
160 170 180 190 200
EKGNFERVKD YKTCLQEFLQ TKGDVAIDYQ VISEKGPAHA KQFEVSIVVN
210 220 230
GAVLSKGLGK SKKLAEQDAA KNALAQLSEV
Length:230
Mass (Da):25,848
Last modified:October 11, 2004 - v1
Checksum:i20076A9055B59B4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA Translation: AAK33526.1
CP000017 Genomic DNA Translation: AAZ51056.1
RefSeqiNP_268805.1, NC_002737.2

Genome annotation databases

EnsemblBacteriaiAAK33526; AAK33526; SPy_0531
AAZ51056; AAZ51056; M5005_Spy0438
GeneIDi900755
KEGGispy:SPy_0531
spz:M5005_Spy0438
PATRICifig|160490.10.peg.453

Similar proteinsi

Entry informationi

Entry nameiRNC_STRP1
AccessioniPrimary (citable) accession number: P66670
Secondary accession number(s): Q490B2, Q9A105
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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