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P66670

- RNC_STRP1

UniProt

P66670 - RNC_STRP1

Protein

Ribonuclease 3

Gene

rnc

Organism
Streptococcus pyogenes serotype M1
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity.By similarity
    CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In this organism endogenous ribonuclease 3 and Cas9 are required for correct coprocessing of pre-crRNA and the trans-encoded small RNA (tracrRNA). Cas9, crRNA and tracrRNA are required for cleavage of invading DNA (PubMed:21455174, PubMed:24270795).2 Publications

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

    Cofactori

    Mg2+.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471MagnesiumUniRule annotation
    Active sitei51 – 511UniRule annotation
    Metal bindingi120 – 1201MagnesiumUniRule annotation
    Active sitei123 – 1231UniRule annotation
    Metal bindingi123 – 1231MagnesiumUniRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonuclease III activity Source: UniProtKB-HAMAP
    3. rRNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. defense response to virus Source: UniProtKB-KW
    2. mRNA processing Source: UniProtKB-HAMAP
    3. rRNA catabolic process Source: InterPro
    4. rRNA processing Source: UniProtKB-HAMAP
    5. tRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Antiviral defense, mRNA processing, rRNA processing, tRNA processing

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding, rRNA-binding

    Enzyme and pathway databases

    BioCyciSPYO160490:GJ81-440-MONOMER.
    SPYO293653:GHFC-493-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease 3UniRule annotation (EC:3.1.26.3UniRule annotation)
    Alternative name(s):
    Ribonuclease IIIUniRule annotation
    Short name:
    RNase IIIUniRule annotation
    Gene namesi
    Name:rncUniRule annotation
    Synonyms:acpA
    Ordered Locus Names:SPy_0531, M5005_Spy0438
    OrganismiStreptococcus pyogenes serotype M1
    Taxonomic identifieri301447 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000000750: Chromosome, UP000002702: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Loss of correct coprocessing of pre-crRNA and tracrRNA. Loss of immunity against a plasmid with homology to CRISPR spacer sequences.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi51 – 511D → A: No processing of pre-crRNA. 1 Publication
    Mutagenesisi154 – 23077Missing: No processing of pre-crRNA. Add
    BLAST

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 230230Ribonuclease 3PRO_0000180443Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi160490.SPy_0531.

    Structurei

    3D structure databases

    ProteinModelPortaliP66670.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 134134RNase IIIUniRule annotationAdd
    BLAST
    Domaini160 – 22970DRBMUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DRBM (double-stranded RNA-binding) domain.UniRule annotation
    Contains 1 RNase III domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0571.
    HOGENOMiHOG000246808.
    KOiK03685.
    OMAiYGFQEAR.
    OrthoDBiEOG6T1WVS.

    Family and domain databases

    Gene3Di1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    HAMAPiMF_00104. RNase_III.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PANTHERiPTHR11207. PTHR11207. 1 hit.
    PfamiPF00035. dsrm. 1 hit.
    PF14622. Ribonucleas_3_3. 1 hit.
    [Graphical view]
    SMARTiSM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view]
    SUPFAMiSSF69065. SSF69065. 1 hit.
    TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
    PROSITEiPS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P66670-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQLEELLST SFDIQFNDLT LLETAFTHTS YANEHRLLNV SHNERLEFLG    50
    DAVLQLIISE YLFAKYPKKT EGDMSKLRSM IVREESLAGF SRFCSFDAYI 100
    KLGKGEEKSG GRRRDTILGD LFEAFLGALL LDKGIDAVRR FLKQVMIPQV 150
    EKGNFERVKD YKTCLQEFLQ TKGDVAIDYQ VISEKGPAHA KQFEVSIVVN 200
    GAVLSKGLGK SKKLAEQDAA KNALAQLSEV 230
    Length:230
    Mass (Da):25,848
    Last modified:October 11, 2004 - v1
    Checksum:i20076A9055B59B4E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004092 Genomic DNA. Translation: AAK33526.1.
    CP000017 Genomic DNA. Translation: AAZ51056.1.
    RefSeqiNP_268805.1. NC_002737.1.
    YP_281801.1. NC_007297.1.

    Genome annotation databases

    EnsemblBacteriaiAAK33526; AAK33526; SPy_0531.
    AAZ51056; AAZ51056; M5005_Spy0438.
    GeneIDi3572493.
    900755.
    KEGGispy:SPy_0531.
    spz:M5005_Spy_0438.
    PATRICi19714769. VBIStrPyo79812_0453.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004092 Genomic DNA. Translation: AAK33526.1 .
    CP000017 Genomic DNA. Translation: AAZ51056.1 .
    RefSeqi NP_268805.1. NC_002737.1.
    YP_281801.1. NC_007297.1.

    3D structure databases

    ProteinModelPortali P66670.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 160490.SPy_0531.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK33526 ; AAK33526 ; SPy_0531 .
    AAZ51056 ; AAZ51056 ; M5005_Spy0438 .
    GeneIDi 3572493.
    900755.
    KEGGi spy:SPy_0531.
    spz:M5005_Spy_0438.
    PATRICi 19714769. VBIStrPyo79812_0453.

    Phylogenomic databases

    eggNOGi COG0571.
    HOGENOMi HOG000246808.
    KOi K03685.
    OMAi YGFQEAR.
    OrthoDBi EOG6T1WVS.

    Enzyme and pathway databases

    BioCyci SPYO160490:GJ81-440-MONOMER.
    SPYO293653:GHFC-493-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1520.10. 1 hit.
    3.30.160.20. 1 hit.
    HAMAPi MF_00104. RNase_III.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    PANTHERi PTHR11207. PTHR11207. 1 hit.
    Pfami PF00035. dsrm. 1 hit.
    PF14622. Ribonucleas_3_3. 1 hit.
    [Graphical view ]
    SMARTi SM00358. DSRM. 1 hit.
    SM00535. RIBOc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69065. SSF69065. 1 hit.
    TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
    PROSITEi PS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700294 / SF370 / Serotype M1.
    2. "Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events."
      Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M.
      J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.
    3. "CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III."
      Deltcheva E., Chylinski K., Sharma C.M., Gonzales K., Chao Y., Pirzada Z.A., Eckert M.R., Vogel J., Charpentier E.
      Nature 471:602-607(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN CRISPR-MEDIATED PLASMID DEFENSE, DISRUPTION PHENOTYPE.
      Strain: ATCC 700294 / SF370 / Serotype M1.
    4. "Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems."
      Fonfara I., Le Rhun A., Chylinski K., Makarova K.S., Lecrivain A.L., Bzdrenga J., Koonin E.V., Charpentier E.
      Nucleic Acids Res. 42:2577-2590(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN CRRNA AND TRACRRNA MATURATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ASP-51 AND 154-ASN--VAL-230.

    Entry informationi

    Entry nameiRNC_STRP1
    AccessioniPrimary (citable) accession number: P66670
    Secondary accession number(s): Q490B2, Q9A105
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3