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P66670

- RNC_STRP1

UniProt

P66670 - RNC_STRP1

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Protein

Ribonuclease 3

Gene

rnc

Organism
Streptococcus pyogenes serotype M1
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon (By similarity).By similarity
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In this organism endogenous ribonuclease 3 and Cas9 are required for correct coprocessing of pre-crRNA and the trans-encoded small RNA (tracrRNA). Cas9, crRNA and tracrRNA are required for cleavage of invading DNA (PubMed:21455174, PubMed:24270795).2 Publications

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471MagnesiumUniRule annotation
Active sitei51 – 511UniRule annotation
Metal bindingi120 – 1201MagnesiumUniRule annotation
Active sitei123 – 1231UniRule annotation
Metal bindingi123 – 1231MagnesiumUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonuclease III activity Source: UniProtKB-HAMAP
  3. rRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. defense response to virus Source: UniProtKB-KW
  2. mRNA processing Source: UniProtKB-HAMAP
  3. rRNA catabolic process Source: InterPro
  4. rRNA processing Source: UniProtKB-HAMAP
  5. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Antiviral defense, mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciSPYO160490:GJ81-440-MONOMER.
SPYO293653:GHFC-493-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3UniRule annotation (EC:3.1.26.3UniRule annotation)
Alternative name(s):
Ribonuclease IIIUniRule annotation
Short name:
RNase IIIUniRule annotation
Gene namesi
Name:rncUniRule annotation
Synonyms:acpA
Ordered Locus Names:SPy_0531, M5005_Spy0438
OrganismiStreptococcus pyogenes serotype M1
Taxonomic identifieri301447 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000750: Chromosome, UP000002702: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Loss of correct coprocessing of pre-crRNA and tracrRNA. Loss of immunity against a plasmid with homology to CRISPR spacer sequences.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi51 – 511D → A: No processing of pre-crRNA. 1 Publication
Mutagenesisi154 – 23077Missing: No processing of pre-crRNA. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Ribonuclease 3PRO_0000180443Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi160490.SPy_0531.

Structurei

3D structure databases

ProteinModelPortaliP66670.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 134134RNase IIIUniRule annotationAdd
BLAST
Domaini160 – 22970DRBMUniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DRBM (double-stranded RNA-binding) domain.UniRule annotation
Contains 1 RNase III domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0571.
HOGENOMiHOG000246808.
KOiK03685.
OMAiYGFQEAR.
OrthoDBiEOG6T1WVS.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P66670 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKQLEELLST SFDIQFNDLT LLETAFTHTS YANEHRLLNV SHNERLEFLG
60 70 80 90 100
DAVLQLIISE YLFAKYPKKT EGDMSKLRSM IVREESLAGF SRFCSFDAYI
110 120 130 140 150
KLGKGEEKSG GRRRDTILGD LFEAFLGALL LDKGIDAVRR FLKQVMIPQV
160 170 180 190 200
EKGNFERVKD YKTCLQEFLQ TKGDVAIDYQ VISEKGPAHA KQFEVSIVVN
210 220 230
GAVLSKGLGK SKKLAEQDAA KNALAQLSEV
Length:230
Mass (Da):25,848
Last modified:October 11, 2004 - v1
Checksum:i20076A9055B59B4E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004092 Genomic DNA. Translation: AAK33526.1.
CP000017 Genomic DNA. Translation: AAZ51056.1.
RefSeqiNP_268805.1. NC_002737.1.
YP_281801.1. NC_007297.1.

Genome annotation databases

EnsemblBacteriaiAAK33526; AAK33526; SPy_0531.
AAZ51056; AAZ51056; M5005_Spy0438.
GeneIDi3572493.
900755.
KEGGispy:SPy_0531.
spz:M5005_Spy_0438.
PATRICi19714769. VBIStrPyo79812_0453.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004092 Genomic DNA. Translation: AAK33526.1 .
CP000017 Genomic DNA. Translation: AAZ51056.1 .
RefSeqi NP_268805.1. NC_002737.1.
YP_281801.1. NC_007297.1.

3D structure databases

ProteinModelPortali P66670.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 160490.SPy_0531.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK33526 ; AAK33526 ; SPy_0531 .
AAZ51056 ; AAZ51056 ; M5005_Spy0438 .
GeneIDi 3572493.
900755.
KEGGi spy:SPy_0531.
spz:M5005_Spy_0438.
PATRICi 19714769. VBIStrPyo79812_0453.

Phylogenomic databases

eggNOGi COG0571.
HOGENOMi HOG000246808.
KOi K03685.
OMAi YGFQEAR.
OrthoDBi EOG6T1WVS.

Enzyme and pathway databases

BioCyci SPYO160490:GJ81-440-MONOMER.
SPYO293653:GHFC-493-MONOMER.

Family and domain databases

Gene3Di 1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPi MF_00104. RNase_III.
InterProi IPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view ]
PANTHERi PTHR11207. PTHR11207. 1 hit.
Pfami PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view ]
SMARTi SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view ]
SUPFAMi SSF69065. SSF69065. 1 hit.
TIGRFAMsi TIGR02191. RNaseIII. 1 hit.
PROSITEi PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700294 / SF370 / Serotype M1.
  2. "Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events."
    Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M.
    J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.
  3. "CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III."
    Deltcheva E., Chylinski K., Sharma C.M., Gonzales K., Chao Y., Pirzada Z.A., Eckert M.R., Vogel J., Charpentier E.
    Nature 471:602-607(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CRISPR-MEDIATED PLASMID DEFENSE, DISRUPTION PHENOTYPE.
    Strain: ATCC 700294 / SF370 / Serotype M1.
  4. "Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems."
    Fonfara I., Le Rhun A., Chylinski K., Makarova K.S., Lecrivain A.L., Bzdrenga J., Koonin E.V., Charpentier E.
    Nucleic Acids Res. 42:2577-2590(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CRRNA AND TRACRRNA MATURATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ASP-51 AND 154-ASN--VAL-230.

Entry informationi

Entry nameiRNC_STRP1
AccessioniPrimary (citable) accession number: P66670
Secondary accession number(s): Q490B2, Q9A105
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: October 29, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3