P66666 (RNC_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	Rv2925c, MT2995
ORF Names:	MTCY338.14c

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs By similarity. HAMAP MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00104
Subcellular location	Cytoplasm By similarity HAMAP MF_00104.
Miscellaneous	Was identified as a high-confidence drug target. HAMAP MF_00104
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro growth Inferred from mutant phenotype. Source: MTBBASE rRNA catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 240

240

Ribonuclease 3 HAMAP MF_00104

PRO_0000180413

Regions

Domain

19 – 134

116

RNase III

Domain

161 – 229

69

DRBM

Secondary structure

1

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240

Helix Strand Turn

Details...

Helix

6 – 12

7

Helix

18 – 24

7

Helix

28 – 33

6

Helix

41 – 62

22

Helix

68 – 79

12

Helix

81 – 90

10

Beta strand

91 – 94

4

Helix

97 – 99

3

Helix

104 – 108

5

Helix

111 – 113

3

Helix

115 – 133

19

Helix

135 – 145

11

Helix

147 – 152

6

Sequences

	Sequence		Length	Mass (Da)
	P66666 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 64C8636742F161DC	FASTA	240	25,399
	10 20 30 40 50 60 MIRSRQPLLD ALGVDLPDEL LSLALTHRSY AYENGGLPTN ERLEFLGDAV LGLTITDALF 70 80 90 100 110 120 HRHPDRSEGD LAKLRASVVN TQALADVARR LCAEGLGVHV LLGRGEANTG GADKSSILAD 130 140 150 160 170 180 GMESLLGAIY LQHGMEKARE VILRLFGPLL DAAPTLGAGL DWKTSLQELT AARGLGAPSY 190 200 210 220 230 240 LVTSTGPDHD KEFTAVVVVM DSEYGSGVGR SKKEAEQKAA AAAWKALEVL DNAMPGKTSA

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BX842581 Genomic DNA. Translation: CAA98974.1.
AE000516 Genomic DNA. Translation: AAK47322.1.

PIR

E70748.

RefSeq

NP_217441.1. NC_000962.2.
NP_337508.1. NC_002755.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2A11	X-ray	2.10	A	1-240	[»]

ProteinModelPortal

P66666.

SMR

P66666. Positions 2-155.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBMYCT00000002463; EBMYCP00000002463; EBMYCG00000002461.
EBMYCT00000072869; EBMYCP00000070928; EBMYCG00000072864.

GeneID

887873.
925280.

GenomeReviews

Gene locus MT2995 in contig AE000516_GR.
Gene locus Rv2925c in contig AL123456_GR.

KEGG

mtc:MT2995.
mtu:Rv2925c.

PATRIC

18128352. VBIMycTub22151_3275.

TIGR

MT2995.

Organism-specific databases

TubercuList

Rv2925c.

Phylogenomic databases

GeneTree

EBGT00050000017732.

HOGENOM

HBG744556.

OMA

ALTHKSF.

PhylomeDB

P66666.

ProtClustDB

PRK00102.

Family and domain databases

HAMAP

MF_00104. RNase_III.
[Tree]

InterPro

IPR001159. Ds-RNA-bd.
IPR014720. dsRNA-bd-like.
IPR000999. RNase_III.
IPR011907. RNase_III_bac.
[Graphical view]

Gene3D

G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit.
G3DSA:1.10.1520.10. RNase_III. 1 hit.

KO

K03685.

PANTHER

PTHR11207. PTHR11207. 1 hit.

Pfam

PF00035. dsrm. 1 hit.
PF00636. Ribonuclease_3. 1 hit.
[Graphical view]

SMART

SM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]

SUPFAM

SSF69065. RNase_III. 1 hit.

TIGRFAMs

TIGR02191. RNaseIII. 1 hit.

PROSITE

PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RNC_MYCTU

Accession

Primary (citable) accession number: P66666
Secondary accession number(s): Q10962

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program