Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P66664 (RNC_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease 3

EC=3.1.26.3
Alternative name(s):
Ribonuclease III
Short name=RNase III
Gene names
Name:rnc
Ordered Locus Names:BMEI1287
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104

Cofactor

Mg2+ By similarity. HAMAP-Rule MF_00104

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00104

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00104.

Sequence similarities

Contains 1 DRBM (double-stranded RNA-binding) domain.

Contains 1 RNase III domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 245245Ribonuclease 3 HAMAP-Rule MF_00104
PRO_0000180378

Regions

Domain19 – 144126RNase III
Domain169 – 23870DRBM

Sites

Active site611 Potential
Active site1331 By similarity
Metal binding571Magnesium By similarity
Metal binding1301Magnesium By similarity
Metal binding1331Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
P66664 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 084ED2D3A3F16BFC

FASTA24527,220
        10         20         30         40         50         60 
MNRTRPLPEI KMVSANKTAS ILEERTGHRF LNLKRLERAL THSSVQAPAR ANYERLEFLG 

        70         80         90        100        110        120 
DRVLGLTVAE MLFEAFPEAS EGELSVRLNA LVNAETCAAI ADEIGLADLI HTGSDIKSLN 

       130        140        150        160        170        180 
DKRLLNVRAD VVEALIATIY LDGGLEAARS FIQRYWKKRS LETGAARRDA KTELQEWAHQ 

       190        200        210        220        230        240 
QGNVHPVYAI LSRSGPDHDP LFLVEVTVKG FAPEKGEGRS KRIAEQSAAE AMLYREGVWK 


RDGSA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL52468.1.
PIRAI3412.
RefSeqNP_540204.1. NC_003317.1.

3D structure databases

ProteinModelPortalP66664.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI1287.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL52468; AAL52468; BMEI1287.
GeneID1196998.
KEGGbme:BMEI1287.
PATRIC17852901. VBIBruMel92729_1442.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000246809.
KOK03685.
OMALTHKSCK.
OrthoDBEOG6T1WVS.
ProtClustDBPRK12371.

Enzyme and pathway databases

BioCycBMEL224914:GCJ0-1327-MONOMER.

Family and domain databases

Gene3D1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPMF_00104. RNase_III.
InterProIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERPTHR11207. PTHR11207. 1 hit.
PfamPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMSSF69065. SSF69065. 1 hit.
TIGRFAMsTIGR02191. RNaseIII. 1 hit.
PROSITEPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNC_BRUME
AccessionPrimary (citable) accession number: P66664
Secondary accession number(s): Q8YG74
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names