Reviewed, UniProtKB/Swiss-Prot P66664 (RNC_BRUME)

Names and origin

Protein names

Recommended name:
    Ribonuclease 3
    EC=3.1.26.3
Alternative name(s):
    Ribonuclease III
      Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	BMEI1287

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs By similarity.
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00104
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA processing Inferred from electronic annotation. Source: HAMAP rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Ribonuclease 3 HAMAP MF_00104		PRO_0000180378
Regions
	Domain	19 – 144	126	RNase III
	Domain	169 – 238	70	DRBM

Sequences

	Sequence		Length	Mass (Da)
	P66664-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 084ED2D3A3F16BFC	FASTA	245	27,220
	10 20 30 40 50 60 MNRTRPLPEI KMVSANKTAS ILEERTGHRF LNLKRLERAL THSSVQAPAR ANYERLEFLG 70 80 90 100 110 120 DRVLGLTVAE MLFEAFPEAS EGELSVRLNA LVNAETCAAI ADEIGLADLI HTGSDIKSLN 130 140 150 160 170 180 DKRLLNVRAD VVEALIATIY LDGGLEAARS FIQRYWKKRS LETGAARRDA KTELQEWAHQ 190 200 210 220 230 240 QGNVHPVYAI LSRSGPDHDP LFLVEVTVKG FAPEKGEGRS KRIAEQSAAE AMLYREGVWK RDGSA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL52468.1.
PIR	AI3412.
RefSeq	NP_540204.1.
3D structure databases
HSSP	HSSP built from PDB template 1DI2 based on UniProtKB Q91836.
ModBase	Search...
Genome annotation databases
GeneID	1196998.
GenomeReviews	Gene locus BMEI1287 in contig AE008917_GR.
KEGG	bme:BMEI1287.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P66664.
OMA	ALTHKSF.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI1287-MON.
BRENDA	3.1.26.3. 277959.
Family and domain databases
HAMAP	MF_00104. [Tree]
InterPro	IPR001159. Ds-RNA_bd. IPR014720. dsRNA-bd-like. IPR000999. RNase_III. IPR011907. RNase_III_bac. [Graphical view]
Gene3D	G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit. G3DSA:1.10.1520.10. RNase_III. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_BRUME

Accession

Primary (citable) accession number: P66664
Secondary accession number(s): Q8YG74

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)