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Protein

3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene

ribB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.UniRule annotation

Catalytic activityi

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Magnesium or manganese.UniRule annotation

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi38Magnesium or manganese 1UniRule annotation1
Metal bindingi38Magnesium or manganese 2UniRule annotation1
Binding sitei42SubstrateUniRule annotation1
Sitei136Essential for catalytic activityUniRule annotation1
Metal bindingi153Magnesium or manganese 2UniRule annotation1
Binding sitei174SubstrateUniRule annotation1
Sitei174Essential for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processRiboflavin biosynthesis
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciSENT99287:G1FZD-3229-MONOMER
BRENDAi4.1.99.12 5542
UniPathwayiUPA00275; UER00399

Names & Taxonomyi

Protein namesi
Recommended name:
3,4-dihydroxy-2-butanone 4-phosphate synthaseUniRule annotation (EC:4.1.99.12UniRule annotation)
Short name:
DHBP synthaseUniRule annotation
Gene namesi
Name:ribBUniRule annotation
Ordered Locus Names:STM3195
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001518101 – 2173,4-dihydroxy-2-butanone 4-phosphate synthaseAdd BLAST217

Proteomic databases

PaxDbiP66032
PRIDEiP66032

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi99287.STM3195

Structurei

Secondary structure

1217
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 24Combined sources13
Beta strandi29 – 32Combined sources4
Turni35 – 38Combined sources4
Beta strandi41 – 46Combined sources6
Turni47 – 49Combined sources3
Helixi52 – 61Combined sources10
Beta strandi67 – 70Combined sources4
Helixi72 – 77Combined sources6
Beta strandi99 – 104Combined sources6
Beta strandi106 – 108Combined sources3
Helixi111 – 122Combined sources12
Helixi128 – 130Combined sources3
Beta strandi131 – 141Combined sources11
Helixi146 – 148Combined sources3
Helixi153 – 164Combined sources12
Beta strandi170 – 176Combined sources7
Beta strandi180 – 182Combined sources3
Helixi185 – 194Combined sources10
Beta strandi198 – 201Combined sources4
Helixi202 – 210Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LQUX-ray2.52A/B1-217[»]
3LRJX-ray2.80A/B/C/D1-217[»]
3LS6X-ray1.86A/B1-217[»]
ProteinModelPortaliP66032
SMRiP66032
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP66032

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 38Substrate bindingUniRule annotation2
Regioni150 – 154Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the DHBP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C66 Bacteria
COG0108 LUCA
HOGENOMiHOG000115444
KOiK02858
OMAiVVCEMLD
PhylomeDBiP66032

Family and domain databases

HAMAPiMF_00180 RibB, 1 hit
InterProiView protein in InterPro
IPR017945 DHBP_synth_RibB-like_a/b_dom
IPR000422 DHBP_synthase_RibB
PfamiView protein in Pfam
PF00926 DHBP_synthase, 1 hit
SUPFAMiSSF55821 SSF55821, 1 hit
TIGRFAMsiTIGR00506 ribB, 1 hit

Sequencei

Sequence statusi: Complete.

P66032-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQTLLSSFG TPFERVELAL DALREGRGVM VLDDEDRENE GDMIFPAETM
60 70 80 90 100
TVEQMALTIR HGSGIVCLCI TEDRRKQLDL PMMVENNTSA YGTGFTVTIE
110 120 130 140 150
AAEGVTTGVS AADRVTTVRA AIKDGAKPSD LNRPGHVFPL RAQAGGVLTR
160 170 180 190 200
GGHTEATIDL MTLAGFKPAG VLCELTNDDG TMARAPECIA FAGQHNMAVV
210
TIEDLVAYRQ AHERKAS
Length:217
Mass (Da):23,310
Last modified:October 11, 2004 - v1
Checksum:iEE07D4C93C3F4EB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006468 Genomic DNA Translation: AAL22069.1
RefSeqiNP_462110.1, NC_003197.2
WP_001076978.1, NC_003197.2

Genome annotation databases

EnsemblBacteriaiAAL22069; AAL22069; STM3195
GeneIDi1254718
KEGGistm:STM3195
PATRICifig|99287.12.peg.3389

Similar proteinsi

Entry informationi

Entry nameiRIBB_SALTY
AccessioniPrimary (citable) accession number: P66032
Secondary accession number(s): Q8XES0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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