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P66015 (RELA_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable GTP pyrophosphokinase

EC=2.7.6.5
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I
Gene names
Name:relA
Ordered Locus Names:Mb2614c
OrganismMycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]
Taxonomic identifier233413 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length790 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp By similarity.

Catalytic activity

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Pathway

Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.

Sequence similarities

Belongs to the RelA/SpoT family.

Contains 1 ACT domain.

Contains 1 HD domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 790790Probable GTP pyrophosphokinase
PRO_0000166552

Regions

Domain105 – 20298HD
Domain714 – 78875ACT

Sequences

Sequence LengthMass (Da)Tools
P66015 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 7A7E669241C0E299

FASTA79087,354
        10         20         30         40         50         60 
MAEDQLTAQA VAPPTEASAA LEPALETPES PVETLKTSIS ASRRVRARLA RRMTAQRSTT 

        70         80         90        100        110        120 
NPVLEPLVAV HREIYPKADL SILQRAYEVA DQRHASQLRQ SGDPYITHPL AVANILAELG 

       130        140        150        160        170        180 
MDTTTLVAAL LHDTVEDTGY TLEALTEEFG EEVGHLVDGV TKLDRVVLGS AAEGETIRKM 

       190        200        210        220        230        240 
ITAMARDPRV LVIKVADRLH NMRTMRFLPP EKQARKARET LEVIAPLAHR LGMASVKWEL 

       250        260        270        280        290        300 
EDLSFAILHP KKYEEIVRLV AGRAPSRDTY LAKVRAEIVN TLTASKIKAT VEGRPKHYWS 

       310        320        330        340        350        360 
IYQKMIVKGR DFDDIHDLVG VRILCDEIRD CYAAVGVVHS LWQPMAGRFK DYIAQPRYGV 

       370        380        390        400        410        420 
YQSLHTTVVG PEGKPLEVQI RTRDMHRTAE YGIAAHWRYK EAKGRNGVLH PHAAAEIDDM 

       430        440        450        460        470        480 
AWMRQLLDWQ REAADPGEFL ESLRYDLAVQ EIFVFTPKGD VITLPTGSTP VDFAYAVHTE 

       490        500        510        520        530        540 
VGHRCIGARV NGRLVALERK LENGEVVEVF TSKAPNAGPS RDWQQFVVSP RAKTKIRQWF 

       550        560        570        580        590        600 
AKERREEALE TGKDAMAREV RRGGLPLQRL VNGESMAAVA RELHYADVSA LYTAIGEGHV 

       610        620        630        640        650        660 
SAKHVVQRLL AELGGIDQAE EELAERSTPA TMPRRPRSTD DVGVSVPGAP GVLTKLAKCC 

       670        680        690        700        710        720 
TPVPGDVIMG FVTRGGGVSV HRTDCTNAAS LQQQAERIIE VLWAPSPSSV FLVAIQVEAL 

       730        740        750        760        770        780 
DRHRLLSDVT RALADEKVNI LSASVTTSGD RVAISRFTFE MGDPKHLGHL LNAVRNVEGV 

       790 
YDVYRVTSAA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248343 Genomic DNA. Translation: CAD94799.1.
RefSeqNP_856260.1. NC_002945.3.

3D structure databases

ProteinModelPortalP66015.
SMRP66015. Positions 65-397, 450-513.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233413.Mb2614c.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD94799; CAD94799; Mb2614c.
GeneID1092024.
KEGGmbo:Mb2614c.
PATRIC18007477. VBIMycBov88188_2875.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0317.
HOGENOMHOG000018301.
KOK00951.
OMALDWLNFV.
OrthoDBEOG6SV551.
ProtClustDBCLSK791939.

Enzyme and pathway databases

UniPathwayUPA00908; UER00884.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR026020. (p)ppGpp_Synthase.
IPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PANTHERPTHR21262:SF1. PTHR21262:SF1. 1 hit.
PfamPF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMSSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00691. spoT_relA. 1 hit.
PROSITEPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRELA_MYCBO
AccessionPrimary (citable) accession number: P66015
Secondary accession number(s): Q50638
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: February 19, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways