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Protein

Probable GTP pyrophosphokinase

Gene

relA

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp (By similarity).By similarity

Catalytic activityi

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Pathwayi

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. GTP binding Source: UniProtKB-KW
  4. GTP diphosphokinase activity Source: UniProtKB-EC
  5. kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. guanosine tetraphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00908; UER00884.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable GTP pyrophosphokinase (EC:2.7.6.5)
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I
Gene namesi
Name:relA
Ordered Locus Names:Mb2614c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 790790Probable GTP pyrophosphokinasePRO_0000166552Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb2614c.

Structurei

3D structure databases

ProteinModelPortaliP66015.
SMRiP66015. Positions 65-397, 450-513.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini105 – 20298HDAdd
BLAST
Domaini714 – 78875ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated
Contains 1 ACT domain.PROSITE-ProRule annotation
Contains 1 HD domain.Curated

Phylogenomic databases

eggNOGiCOG0317.
HOGENOMiHOG000018301.
KOiK00951.
OMAiLDWLNFV.
OrthoDBiEOG6SV551.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P66015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEDQLTAQA VAPPTEASAA LEPALETPES PVETLKTSIS ASRRVRARLA
60 70 80 90 100
RRMTAQRSTT NPVLEPLVAV HREIYPKADL SILQRAYEVA DQRHASQLRQ
110 120 130 140 150
SGDPYITHPL AVANILAELG MDTTTLVAAL LHDTVEDTGY TLEALTEEFG
160 170 180 190 200
EEVGHLVDGV TKLDRVVLGS AAEGETIRKM ITAMARDPRV LVIKVADRLH
210 220 230 240 250
NMRTMRFLPP EKQARKARET LEVIAPLAHR LGMASVKWEL EDLSFAILHP
260 270 280 290 300
KKYEEIVRLV AGRAPSRDTY LAKVRAEIVN TLTASKIKAT VEGRPKHYWS
310 320 330 340 350
IYQKMIVKGR DFDDIHDLVG VRILCDEIRD CYAAVGVVHS LWQPMAGRFK
360 370 380 390 400
DYIAQPRYGV YQSLHTTVVG PEGKPLEVQI RTRDMHRTAE YGIAAHWRYK
410 420 430 440 450
EAKGRNGVLH PHAAAEIDDM AWMRQLLDWQ REAADPGEFL ESLRYDLAVQ
460 470 480 490 500
EIFVFTPKGD VITLPTGSTP VDFAYAVHTE VGHRCIGARV NGRLVALERK
510 520 530 540 550
LENGEVVEVF TSKAPNAGPS RDWQQFVVSP RAKTKIRQWF AKERREEALE
560 570 580 590 600
TGKDAMAREV RRGGLPLQRL VNGESMAAVA RELHYADVSA LYTAIGEGHV
610 620 630 640 650
SAKHVVQRLL AELGGIDQAE EELAERSTPA TMPRRPRSTD DVGVSVPGAP
660 670 680 690 700
GVLTKLAKCC TPVPGDVIMG FVTRGGGVSV HRTDCTNAAS LQQQAERIIE
710 720 730 740 750
VLWAPSPSSV FLVAIQVEAL DRHRLLSDVT RALADEKVNI LSASVTTSGD
760 770 780 790
RVAISRFTFE MGDPKHLGHL LNAVRNVEGV YDVYRVTSAA
Length:790
Mass (Da):87,354
Last modified:October 11, 2004 - v1
Checksum:i7A7E669241C0E299
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO43878.1.
RefSeqiNP_856260.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO43878; CDO43878; Mb2614c.
GeneIDi1092024.
KEGGimbo:Mb2614c.
PATRICi18007477. VBIMycBov88188_2875.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO43878.1.
RefSeqiNP_856260.1. NC_002945.3.

3D structure databases

ProteinModelPortaliP66015.
SMRiP66015. Positions 65-397, 450-513.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi233413.Mb2614c.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO43878; CDO43878; Mb2614c.
GeneIDi1092024.
KEGGimbo:Mb2614c.
PATRICi18007477. VBIMycBov88188_2875.

Phylogenomic databases

eggNOGiCOG0317.
HOGENOMiHOG000018301.
KOiK00951.
OMAiLDWLNFV.
OrthoDBiEOG6SV551.

Enzyme and pathway databases

UniPathwayiUPA00908; UER00884.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiRELA_MYCBO
AccessioniPrimary (citable) accession number: P66015
Secondary accession number(s): Q50638, X2BLD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: April 1, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.