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Protein

DNA repair protein RadA

Gene

radA

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 27C4-typeUniRule annotationAdd BLAST18
Nucleotide bindingi95 – 102ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Hydrolase
Biological processDNA damage, DNA repair, Stress response
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMBOV233413:G1GT4-3793-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RadAUniRule annotation (EC:3.6.4.-UniRule annotation)
Alternative name(s):
Branch migration protein RadAUniRule annotation
Gene namesi
Name:radAUniRule annotation
Ordered Locus Names:BQ2027_MB3616
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001879331 – 480DNA repair protein RadAAdd BLAST480

Structurei

3D structure databases

ProteinModelPortaliP65954
SMRiP65954
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni353 – 480Lon-protease-likeUniRule annotationAdd BLAST128

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi254 – 258RadA KNRFG motifUniRule annotation5

Domaini

Has a putative N-terminal zinc-finger, a middle region with homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease.UniRule annotation

Sequence similaritiesi

Belongs to the RecA family. RadA subfamily.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 27C4-typeUniRule annotationAdd BLAST18

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000218329
OMAiSQVREIT

Family and domain databases

Gene3Di3.30.230.10, 1 hit
HAMAPiMF_01498 RadA_bact, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR004504 DNA_repair_RadA
IPR027417 P-loop_NTPase
IPR020588 RecA_ATP-bd
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF54211 SSF54211, 2 hits
TIGRFAMsiTIGR00416 sms, 1 hit
PROSITEiView protein in PROSITE
PS50162 RECA_2, 1 hit

Sequencei

Sequence statusi: Complete.

P65954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANARSQYRC SECRHVSAKW VGRCLECGRW GTVDEVAVLS AVGGTRRRSV
60 70 80 90 100
APASGAVPIS AVDAHRTRPC PTGIDELDRV LGGGIVPGSV TLLAGDPGVG
110 120 130 140 150
KSTLLLEVAH RWAQSGRRAL YVSGEESAGQ IRLRADRIGC GTEVEEIYLA
160 170 180 190 200
AQSDVHTVLD QIETVQPALV IVDSVQTMST SEADGVTGGV TQVRAVTAAL
210 220 230 240 250
TAAAKANEVA LILVGHVTKD GAIAGPRSLE HLVDVVLHFE GDRNGALRMV
260 270 280 290 300
RGVKNRFGAA DEVGCFLLHD NGIDGIVDPS NLFLDQRPTP VAGTAITVTL
310 320 330 340 350
DGKRPLVGEV QALLATPCGG SPRRAVSGIH QARAAMIAAV LEKHARLAIA
360 370 380 390 400
VNDIYLSTVG GMRLTEPSAD LAVAIALASA YANLPLPTTA VMIGEVGLAG
410 420 430 440 450
DIRRVNGMAR RLSEAARQGF TIALVPPSDD PVPPGMHALR ASTIVAALQY
460 470 480
MVDIADHRGT TLATPPSHSG TGHVPLGRGT
Length:480
Mass (Da):49,913
Last modified:October 11, 2004 - v1
Checksum:iDDECEA049F7099BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LT708304 Genomic DNA Translation: SIU02243.1
RefSeqiNP_857255.1, NC_002945.3
WP_003419487.1, NC_002945.4

Genome annotation databases

EnsemblBacteriaiCDO44886; CDO44886; Mb3616
PATRICifig|233413.5.peg.3962

Similar proteinsi

Entry informationi

Entry nameiRADA_MYCBO
AccessioniPrimary (citable) accession number: P65954
Secondary accession number(s): A0A1R3Y4J3, O53570, X2BPV8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: April 25, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health