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Protein

DNA repair protein RadA

Gene

radA

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri10 – 2718C4-typeUniRule annotationAdd
BLAST
Nucleotide bindingi95 – 1028ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, Stress response

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RadAUniRule annotation (EC:3.6.4.-UniRule annotation)
Alternative name(s):
Branch migration protein RadAUniRule annotation
Gene namesi
Name:radAUniRule annotation
Ordered Locus Names:Mb3616
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480DNA repair protein RadAPRO_0000187933Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP65954.
SMRiP65954. Positions 67-248.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni353 – 480128Lon-protease-likeUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi254 – 2585RadA KNRFG motifUniRule annotation

Domaini

Has a putative N-terminal zinc-finger, a middle region with homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease.UniRule annotation

Sequence similaritiesi

Belongs to the RecA family. RadA subfamily.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri10 – 2718C4-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000218329.
KOiK04485.
OMAiEVGCFEL.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01498. RadA_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004504. DNA_repair_RadA.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 2 hits.
TIGRFAMsiTIGR00416. sms. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P65954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANARSQYRC SECRHVSAKW VGRCLECGRW GTVDEVAVLS AVGGTRRRSV
60 70 80 90 100
APASGAVPIS AVDAHRTRPC PTGIDELDRV LGGGIVPGSV TLLAGDPGVG
110 120 130 140 150
KSTLLLEVAH RWAQSGRRAL YVSGEESAGQ IRLRADRIGC GTEVEEIYLA
160 170 180 190 200
AQSDVHTVLD QIETVQPALV IVDSVQTMST SEADGVTGGV TQVRAVTAAL
210 220 230 240 250
TAAAKANEVA LILVGHVTKD GAIAGPRSLE HLVDVVLHFE GDRNGALRMV
260 270 280 290 300
RGVKNRFGAA DEVGCFLLHD NGIDGIVDPS NLFLDQRPTP VAGTAITVTL
310 320 330 340 350
DGKRPLVGEV QALLATPCGG SPRRAVSGIH QARAAMIAAV LEKHARLAIA
360 370 380 390 400
VNDIYLSTVG GMRLTEPSAD LAVAIALASA YANLPLPTTA VMIGEVGLAG
410 420 430 440 450
DIRRVNGMAR RLSEAARQGF TIALVPPSDD PVPPGMHALR ASTIVAALQY
460 470 480
MVDIADHRGT TLATPPSHSG TGHVPLGRGT
Length:480
Mass (Da):49,913
Last modified:October 11, 2004 - v1
Checksum:iDDECEA049F7099BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44886.1.
RefSeqiNP_857255.1. NC_002945.3.
WP_003419487.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO44886; CDO44886; Mb3616.
GeneIDi1093449.
KEGGimbo:Mb3616.
PATRICi18009669. VBIMycBov88188_3962.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44886.1.
RefSeqiNP_857255.1. NC_002945.3.
WP_003419487.1. NC_002945.3.

3D structure databases

ProteinModelPortaliP65954.
SMRiP65954. Positions 67-248.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO44886; CDO44886; Mb3616.
GeneIDi1093449.
KEGGimbo:Mb3616.
PATRICi18009669. VBIMycBov88188_3962.

Phylogenomic databases

HOGENOMiHOG000218329.
KOiK04485.
OMAiEVGCFEL.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01498. RadA_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004504. DNA_repair_RadA.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 2 hits.
TIGRFAMsiTIGR00416. sms. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRADA_MYCBO
AccessioniPrimary (citable) accession number: P65954
Secondary accession number(s): O53570, X2BPV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.