Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Gene

queA

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA).UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + 7-aminomethyl-7-carbaguanosine(34) in tRNA = L-methionine + adenine + epoxyqueuosine(34) in tRNA.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. isomerase activity Source: UniProtKB-HAMAP
  2. transferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine:tRNA ribosyltransferase-isomeraseUniRule annotation (EC:2.4.99.17UniRule annotation)
Alternative name(s):
Queuosine biosynthesis protein QueAUniRule annotation
Gene namesi
Name:queAUniRule annotation
Ordered Locus Names:BMEI0889
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363S-adenosylmethionine:tRNA ribosyltransferase-isomerasePRO_0000165386Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224914.BMEI0889.

Structurei

3D structure databases

ProteinModelPortaliP65948.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the QueA family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000004401.
KOiK07568.
OMAiHAEYAEV.
OrthoDBiEOG6X6RBB.

Family and domain databases

HAMAPiMF_00113. QueA.
InterProiIPR003699. QueA.
[Graphical view]
PfamiPF02547. Queuosine_synth. 1 hit.
[Graphical view]
SUPFAMiSSF111337. SSF111337. 1 hit.
TIGRFAMsiTIGR00113. queA. 1 hit.

Sequencei

Sequence statusi: Complete.

P65948-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVDLFDFDL PEERIALRPV EPRDHAKLLH VRPGEPFEDR HVYDLPDLLQ
60 70 80 90 100
PGDALVFNDT KVIPAQLEGM RERTGNISQV SATLHMRVGP DRWKAFLRPA
110 120 130 140 150
KRVKEGDRIR FGHSGTSCFL GTLDATVAEK GDSGEALLVF DLSGAVLDEA
160 170 180 190 200
IAAVGHIPLP PYIASKRPED ERDRKDYQTV YAREEGAVAA PTAGLHFTPD
210 220 230 240 250
LLEKIKARGI EEHFVTLHVG AGTFLPVKAD DTGDHKMHAE IGHVSQRTAS
260 270 280 290 300
ALNAVHERGG RIICVGTTSL RLIESATGED GVVRPWSGAT DIFITPGYRF
310 320 330 340 350
RAVDLLMTNF HLPRSTLFML VSAFSGLDTM HAAYNYAIAD GYRFYSYGDA
360
SLLERIDHDR HSA
Length:363
Mass (Da):40,174
Last modified:October 11, 2004 - v1
Checksum:i972A658AA3E91CFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52070.1.
PIRiAC3363.
RefSeqiNP_539806.1. NC_003317.1.

Genome annotation databases

EnsemblBacteriaiAAL52070; AAL52070; BMEI0889.
GeneIDi1196600.
KEGGibme:BMEI0889.
PATRICi17801966. VBIBruMel146950_3303.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL52070.1.
PIRiAC3363.
RefSeqiNP_539806.1. NC_003317.1.

3D structure databases

ProteinModelPortaliP65948.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BMEI0889.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL52070; AAL52070; BMEI0889.
GeneIDi1196600.
KEGGibme:BMEI0889.
PATRICi17801966. VBIBruMel146950_3303.

Phylogenomic databases

HOGENOMiHOG000004401.
KOiK07568.
OMAiHAEYAEV.
OrthoDBiEOG6X6RBB.

Enzyme and pathway databases

UniPathwayiUPA00392.

Family and domain databases

HAMAPiMF_00113. QueA.
InterProiIPR003699. QueA.
[Graphical view]
PfamiPF02547. Queuosine_synth. 1 hit.
[Graphical view]
SUPFAMiSSF111337. SSF111337. 1 hit.
TIGRFAMsiTIGR00113. queA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiQUEA_BRUME
AccessioniPrimary (citable) accession number: P65948
Secondary accession number(s): Q8YHB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: January 7, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.