P65948 (QUEA_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-adenosylmethionine:tRNA ribosyltransferase-isomerase
EC=2.4.99.17

Alternative name(s):
Queuosine biosynthesis protein QueA

Gene names

Name:	queA
Ordered Locus Names:	BMEI0889

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) By similarity. HAMAP-Rule MF_00113
Catalytic activity	S-adenosyl-L-methionine + 7-aminomethyl-7-carbaguanosine34 in tRNA = L-methionine + adenine + epoxyqueuosine34 in tRNA. HAMAP-Rule MF_00113
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00113
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the QueA family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isomerase activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	S-adenosylmethionine:tRNA ribosyltransferase-isomerase HAMAP-Rule MF_00113		PRO_0000165386

Sequences

	Sequence		Length	Mass (Da)
	P65948 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 972A658AA3E91CFD	FASTA	363	40,174
	10 20 30 40 50 60 MRVDLFDFDL PEERIALRPV EPRDHAKLLH VRPGEPFEDR HVYDLPDLLQ PGDALVFNDT 70 80 90 100 110 120 KVIPAQLEGM RERTGNISQV SATLHMRVGP DRWKAFLRPA KRVKEGDRIR FGHSGTSCFL 130 140 150 160 170 180 GTLDATVAEK GDSGEALLVF DLSGAVLDEA IAAVGHIPLP PYIASKRPED ERDRKDYQTV 190 200 210 220 230 240 YAREEGAVAA PTAGLHFTPD LLEKIKARGI EEHFVTLHVG AGTFLPVKAD DTGDHKMHAE 250 260 270 280 290 300 IGHVSQRTAS ALNAVHERGG RIICVGTTSL RLIESATGED GVVRPWSGAT DIFITPGYRF 310 320 330 340 350 360 RAVDLLMTNF HLPRSTLFML VSAFSGLDTM HAAYNYAIAD GYRFYSYGDA SLLERIDHDR HSA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52070.1.
PIR	AC3363.
RefSeq	NP_539806.1. NC_003317.1.
3D structure databases
ProteinModelPortal	P65948.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI0889.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL52070; AAL52070; BMEI0889.
GeneID	1196600.
KEGG	bme:BMEI0889.
PATRIC	17801966. VBIBruMel146950_3303.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000004401.
KO	K07568.
OMA	HRMHSEW.
ProtClustDB	PRK00147.
Enzyme and pathway databases
UniPathway	UPA00392.
Family and domain databases
HAMAP	MF_00113. QueA.
InterPro	IPR003699. Queuosine_synth. [Graphical view]
Pfam	PF02547. Queuosine_synth. 1 hit. [Graphical view]
SUPFAM	SSF111337. Queuosine_synth. 1 hit.
TIGRFAMs	TIGR00113. queA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEA_BRUME

Accession

Primary (citable) accession number: P65948
Secondary accession number(s): Q8YHB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program