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P65948 (QUEA_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
S-adenosylmethionine:tRNA ribosyltransferase-isomerase

EC=2.4.99.17
Alternative name(s):
Queuosine biosynthesis protein QueA
Gene names
Name:queA
Ordered Locus Names:BMEI0889
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length363 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) By similarity. HAMAP-Rule MF_00113

Catalytic activity

S-adenosyl-L-methionine + 7-aminomethyl-7-carbaguanosine34 in tRNA = L-methionine + adenine + epoxyqueuosine34 in tRNA. HAMAP-Rule MF_00113

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00113

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00113

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00113.

Sequence similarities

Belongs to the QueA family.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
   Cellular componentCytoplasm
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processqueuosine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionisomerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

transferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 363363S-adenosylmethionine:tRNA ribosyltransferase-isomerase HAMAP-Rule MF_00113
PRO_0000165386

Sequences

Sequence LengthMass (Da)Tools
P65948 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 972A658AA3E91CFD

FASTA36340,174
        10         20         30         40         50         60 
MRVDLFDFDL PEERIALRPV EPRDHAKLLH VRPGEPFEDR HVYDLPDLLQ PGDALVFNDT 

        70         80         90        100        110        120 
KVIPAQLEGM RERTGNISQV SATLHMRVGP DRWKAFLRPA KRVKEGDRIR FGHSGTSCFL 

       130        140        150        160        170        180 
GTLDATVAEK GDSGEALLVF DLSGAVLDEA IAAVGHIPLP PYIASKRPED ERDRKDYQTV 

       190        200        210        220        230        240 
YAREEGAVAA PTAGLHFTPD LLEKIKARGI EEHFVTLHVG AGTFLPVKAD DTGDHKMHAE 

       250        260        270        280        290        300 
IGHVSQRTAS ALNAVHERGG RIICVGTTSL RLIESATGED GVVRPWSGAT DIFITPGYRF 

       310        320        330        340        350        360 
RAVDLLMTNF HLPRSTLFML VSAFSGLDTM HAAYNYAIAD GYRFYSYGDA SLLERIDHDR 


HSA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL52070.1.
PIRAC3363.
RefSeqNP_539806.1. NC_003317.1.

3D structure databases

ProteinModelPortalP65948.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI0889.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL52070; AAL52070; BMEI0889.
GeneID1196600.
KEGGbme:BMEI0889.
PATRIC17801966. VBIBruMel146950_3303.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000004401.
KOK07568.
OMAHAEYAEV.
OrthoDBEOG6X6RBB.
ProtClustDBPRK00147.

Enzyme and pathway databases

UniPathwayUPA00392.

Family and domain databases

HAMAPMF_00113. QueA.
InterProIPR003699. QueA.
[Graphical view]
PfamPF02547. Queuosine_synth. 1 hit.
[Graphical view]
SUPFAMSSF111337. SSF111337. 1 hit.
TIGRFAMsTIGR00113. queA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQUEA_BRUME
AccessionPrimary (citable) accession number: P65948
Secondary accession number(s): Q8YHB3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: February 19, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names