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Protein

Uridylate kinase

Gene

pyrH

Organism
Staphylococcus aureus (strain MW2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of UMP to UDP.UniRule annotation

Catalytic activityi

ATP + UMP = ADP + UDP.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP. Inhibited by UTP.UniRule annotation

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UDP from UMP (UMPK route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Uridylate kinase (pyrH)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP from UMP (UMPK route), the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551UMP; via amide nitrogenUniRule annotation
Binding sitei56 – 561ATP; via amide nitrogenUniRule annotation
Binding sitei60 – 601ATPUniRule annotation
Binding sitei75 – 751UMPUniRule annotation
Binding sitei164 – 1641ATPUniRule annotation
Binding sitei170 – 1701ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei173 – 1731ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 164ATPUniRule annotation
Nucleotide bindingi136 – 1438UMPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR196620:GJ9Z-1166-MONOMER.
UniPathwayiUPA00159; UER00275.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridylate kinaseUniRule annotation (EC:2.7.4.22UniRule annotation)
Short name:
UKUniRule annotation
Alternative name(s):
Uridine monophosphate kinaseUniRule annotation
Short name:
UMP kinaseUniRule annotation
Short name:
UMPKUniRule annotation
Gene namesi
Name:pyrHUniRule annotation
Synonyms:smbA
Ordered Locus Names:MW1141
OrganismiStaphylococcus aureus (strain MW2)
Taxonomic identifieri196620 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000418 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240Uridylate kinasePRO_0000143886Add
BLAST

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP65937.
SMRiP65937. Positions 6-239.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 266Involved in allosteric activation by GTPUniRule annotation

Sequence similaritiesi

Belongs to the UMP kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000047187.
OMAiRSRADYM.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_01220_B. PyrH_B. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR011817. Uridylate_kinase.
IPR015963. Uridylate_kinase_bac.
[Graphical view]
PANTHERiPTHR21499:SF23. PTHR21499:SF23. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF005650. Uridylate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR02075. pyrH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

P65937-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQISKYKRV VLKLSGEALA GEKGFGINPV IIKSVAEQVA EVAKMDCEIA
60 70 80 90 100
VIVGGGNIWR GKTGSDLGMD RGTADYMGML ATVMNALALQ DSLEQLDCDT
110 120 130 140 150
RVLTSIEMKQ VAEPYIRRRA IRHLEKKRVV IFAAGIGNPY FSTDTTAALR
160 170 180 190 200
AAEVEADVIL MGKNNVDGVY SADPKVNKDA VKYEHLTHIQ MLQEGLQVMD
210 220 230 240
STASSFCMDN NIPLTVFSIM EEGNIKRAVM GEKIGTLITK
Length:240
Mass (Da):26,145
Last modified:October 11, 2004 - v1
Checksum:iFD4099DEC5F87C11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB95006.1.
RefSeqiWP_000057330.1. NC_003923.1.

Genome annotation databases

EnsemblBacteriaiBAB95006; BAB95006; BAB95006.
PATRICi19568878. VBIStaAur44266_1200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB95006.1.
RefSeqiWP_000057330.1. NC_003923.1.

3D structure databases

ProteinModelPortaliP65937.
SMRiP65937. Positions 6-239.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB95006; BAB95006; BAB95006.
PATRICi19568878. VBIStaAur44266_1200.

Phylogenomic databases

HOGENOMiHOG000047187.
OMAiRSRADYM.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00275.
BioCyciSAUR196620:GJ9Z-1166-MONOMER.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_01220_B. PyrH_B. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR011817. Uridylate_kinase.
IPR015963. Uridylate_kinase_bac.
[Graphical view]
PANTHERiPTHR21499:SF23. PTHR21499:SF23. 1 hit.
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF005650. Uridylate_kin. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR02075. pyrH_bact. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPYRH_STAAW
AccessioniPrimary (citable) accession number: P65937
Secondary accession number(s): P59006, Q99UL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.